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- EMDB-6774: Cryo-EM structure of human respiratory complex III (cytochrome bc... -
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Basic information
Entry | Database: EMDB / ID: EMD-6774 | |||||||||
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Title | Cryo-EM structure of human respiratory complex III (cytochrome bc1 complex) | |||||||||
![]() | This map was obtained by sub-region refinement. | |||||||||
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Function / homology | ![]() Complex III assembly / Complex III assembly / Complex III assembly / Complex III assembly / Complex III assembly / Complex III assembly / Complex III assembly / Complex III assembly / Complex III assembly / subthalamus development ...Complex III assembly / Complex III assembly / Complex III assembly / Complex III assembly / Complex III assembly / Complex III assembly / Complex III assembly / Complex III assembly / Complex III assembly / subthalamus development / pons development / cerebellar Purkinje cell layer development / response to mercury ion / Respiratory electron transport / pyramidal neuron development / thalamus development / mitochondrial respiratory chain complex III assembly / Mitochondrial protein import / cellular respiration / respiratory chain complex / response to copper ion / response to alkaloid / respiratory chain complex III / oxidative phosphorylation / response to glucagon / quinol-cytochrome-c reductase / ubiquinol-cytochrome-c reductase activity / mitochondrial electron transport, ubiquinol to cytochrome c / hypothalamus development / response to cobalamin / midbrain development / electron transport coupled proton transport / response to hyperoxia / animal organ regeneration / response to cadmium ion / Mitochondrial protein degradation / aerobic respiration / respiratory electron transport chain / response to activity / generation of precursor metabolites and energy / hippocampus development / metalloendopeptidase activity / 2 iron, 2 sulfur cluster binding / response to calcium ion / response to toxic substance / response to ethanol / oxidoreductase activity / electron transfer activity / response to hypoxia / mitochondrial inner membrane / mitochondrial matrix / response to xenobiotic stimulus / ubiquitin protein ligase binding / heme binding / protein-containing complex binding / mitochondrion / proteolysis / nucleoplasm / nucleus / membrane / metal ion binding Similarity search - Function | |||||||||
Biological species | ![]() | |||||||||
Method | single particle reconstruction / cryo EM / Resolution: 3.4 Å | |||||||||
![]() | Gu J / Wu M / Yang M | |||||||||
![]() | ![]() Title: Architecture of Human Mitochondrial Respiratory Megacomplex IIIIIV. Authors: Runyu Guo / Shuai Zong / Meng Wu / Jinke Gu / Maojun Yang / ![]() Abstract: The respiratory megacomplex represents the highest-order assembly of respiratory chain complexes, and it allows mitochondria to respond to energy-requiring conditions. To understand its architecture, ...The respiratory megacomplex represents the highest-order assembly of respiratory chain complexes, and it allows mitochondria to respond to energy-requiring conditions. To understand its architecture, we examined the human respiratory chain megacomplex-IIIIIV (MCIIIIIV) with 140 subunits and a subset of associated cofactors using cryo-electron microscopy. The MCIIIIIV forms a circular structure with the dimeric CIII located in the center, where it is surrounded by two copies each of CI and CIV. Two cytochrome c (Cyt.c) molecules are positioned to accept electrons on the surface of the c state CIII dimer. Analyses indicate that CII could insert into the gaps between CI and CIV to form a closed ring, which we termed the electron transport chain supercomplex. The structure not only reveals the precise assignment of individual subunits of human CI and CIII, but also enables future in-depth analysis of the electron transport chain as a whole. | |||||||||
History |
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Structure visualization
Movie |
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Structure viewer | EM map: ![]() ![]() ![]() |
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 22.1 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 10 KB 10 KB | Display Display | ![]() |
Images | ![]() | 18.2 KB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Validation report
Summary document | ![]() | 312.8 KB | Display | ![]() |
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Full document | ![]() | 312.3 KB | Display | |
Data in XML | ![]() | 7.6 KB | Display | |
Arichive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
Related structure data | ![]() 5xteMC ![]() 6771C ![]() 6772C ![]() 6773C ![]() 6775C ![]() 6776C ![]() 5xtbC ![]() 5xtcC ![]() 5xtdC ![]() 5xthC ![]() 5xtiC M: atomic model generated by this map C: citing same article ( |
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Similar structure data |
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Links
EMDB pages | ![]() ![]() |
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Related items in Molecule of the Month |
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Map
File | ![]() | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Annotation | This map was obtained by sub-region refinement. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Projections & slices | Image control
Images are generated by Spider. | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Voxel size | X=Y=Z: 1.083 Å | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Details | EMDB XML:
CCP4 map header:
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-Supplemental data
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Sample components
-Entire : Human respiratory complex III
Entire | Name: Human respiratory complex III |
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Components |
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-Supramolecule #1: Human respiratory complex III
Supramolecule | Name: Human respiratory complex III / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#11 / Details: Human cytochrome bc1 complex |
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Source (natural) | Organism: ![]() |
Molecular weight | Experimental: 500 KDa |
-Experimental details
-Structure determination
Method | cryo EM |
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![]() | single particle reconstruction |
Aggregation state | particle |
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Sample preparation
Buffer | pH: 7.4 |
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Vitrification | Cryogen name: ETHANE |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Image recording | Film or detector model: FEI FALCON II (4k x 4k) / Average electron dose: 1.25 e/Å2 |
Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | Illumination mode: OTHER / Imaging mode: BRIGHT FIELD |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |
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Image processing
CTF correction | Software - Name: CTFFIND (ver. 3.0) |
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Final reconstruction | Applied symmetry - Point group: C1 (asymmetric) / Resolution.type: BY AUTHOR / Resolution: 3.4 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: RELION (ver. 1.4) / Number images used: 167761 |
Initial angle assignment | Type: RANDOM ASSIGNMENT / Software - Name: RELION (ver. 1.4) |
Final angle assignment | Type: RANDOM ASSIGNMENT / Software - Name: RELION (ver. 1.4) |