5X67
Human thymidylate synthase in complex with dUMP and nolatrexed
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000900 | molecular_function | mRNA regulatory element binding translation repressor activity |
| A | 0004799 | molecular_function | thymidylate synthase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005542 | molecular_function | folic acid binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005657 | cellular_component | replication fork |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005743 | cellular_component | mitochondrial inner membrane |
| A | 0005759 | cellular_component | mitochondrial matrix |
| A | 0005829 | cellular_component | cytosol |
| A | 0006207 | biological_process | 'de novo' pyrimidine nucleobase biosynthetic process |
| A | 0006231 | biological_process | dTMP biosynthetic process |
| A | 0006235 | biological_process | dTTP biosynthetic process |
| A | 0006260 | biological_process | DNA replication |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0009165 | biological_process | nucleotide biosynthetic process |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| A | 0017148 | biological_process | negative regulation of translation |
| A | 0032259 | biological_process | methylation |
| A | 0035999 | biological_process | tetrahydrofolate interconversion |
| A | 0046653 | biological_process | tetrahydrofolate metabolic process |
| A | 0071897 | biological_process | DNA biosynthetic process |
| A | 1990825 | molecular_function | sequence-specific mRNA binding |
| B | 0000900 | molecular_function | mRNA regulatory element binding translation repressor activity |
| B | 0004799 | molecular_function | thymidylate synthase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005542 | molecular_function | folic acid binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005657 | cellular_component | replication fork |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005743 | cellular_component | mitochondrial inner membrane |
| B | 0005759 | cellular_component | mitochondrial matrix |
| B | 0005829 | cellular_component | cytosol |
| B | 0006207 | biological_process | 'de novo' pyrimidine nucleobase biosynthetic process |
| B | 0006231 | biological_process | dTMP biosynthetic process |
| B | 0006235 | biological_process | dTTP biosynthetic process |
| B | 0006260 | biological_process | DNA replication |
| B | 0008168 | molecular_function | methyltransferase activity |
| B | 0009165 | biological_process | nucleotide biosynthetic process |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| B | 0017148 | biological_process | negative regulation of translation |
| B | 0032259 | biological_process | methylation |
| B | 0035999 | biological_process | tetrahydrofolate interconversion |
| B | 0046653 | biological_process | tetrahydrofolate metabolic process |
| B | 0071897 | biological_process | DNA biosynthetic process |
| B | 1990825 | molecular_function | sequence-specific mRNA binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 15 |
| Details | binding site for residue UMP A 401 |
| Chain | Residue |
| A | ARG50 |
| A | TYR258 |
| A | 7Z9402 |
| A | HOH527 |
| A | HOH529 |
| B | ARG175 |
| B | ARG176 |
| A | CYS195 |
| A | HIS196 |
| A | GLN214 |
| A | ARG215 |
| A | SER216 |
| A | ASP218 |
| A | ASN226 |
| A | HIS256 |
| site_id | AC2 |
| Number of Residues | 12 |
| Details | binding site for residue 7Z9 A 402 |
| Chain | Residue |
| A | ILE108 |
| A | TRP109 |
| A | ASN112 |
| A | ASP218 |
| A | LEU221 |
| A | GLY222 |
| A | PHE225 |
| A | MET311 |
| A | ALA312 |
| A | UMP401 |
| A | HOH531 |
| A | HOH555 |
| site_id | AC3 |
| Number of Residues | 16 |
| Details | binding site for residue UMP B 401 |
| Chain | Residue |
| A | ARG175 |
| A | ARG176 |
| B | ARG50 |
| B | CYS195 |
| B | HIS196 |
| B | GLN214 |
| B | ARG215 |
| B | SER216 |
| B | GLY217 |
| B | ASP218 |
| B | ASN226 |
| B | HIS256 |
| B | TYR258 |
| B | 7Z9402 |
| B | HOH522 |
| B | HOH572 |
| site_id | AC4 |
| Number of Residues | 11 |
| Details | binding site for residue 7Z9 B 402 |
| Chain | Residue |
| B | ILE108 |
| B | TRP109 |
| B | ASN112 |
| B | ASP218 |
| B | LEU221 |
| B | GLY222 |
| B | PHE225 |
| B | MET311 |
| B | ALA312 |
| B | UMP401 |
| B | HOH518 |
Functional Information from PROSITE/UniProt
| site_id | PS00091 |
| Number of Residues | 29 |
| Details | THYMIDYLATE_SYNTHASE Thymidylate synthase active site. RriImcaWNprdlplma.....LpPCHalcQFyV |
| Chain | Residue | Details |
| A | ARG175-VAL203 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 2 |
| Details | Active site: {"description":"Nucleophile","evidences":[{"source":"UniProtKB","id":"P0A884","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Binding site: {"description":"in other chain","evidences":[{"source":"UniProtKB","id":"P0A884","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"P45352","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 12 |
| Details | Binding site: {"description":"in other chain","evidences":[{"source":"UniProtKB","id":"P45352","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"P0A884","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"19690332","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 8 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
