Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

5US1

Crystal structure of aminoglycoside acetyltransferase AAC(2')-Ia in complex with N2'-acetylgentamicin C1A and coenzyme A

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0016740	molecular_function	transferase activity
A	0016746	molecular_function	acyltransferase activity
A	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
A	0046677	biological_process	response to antibiotic
A	0047921	molecular_function	aminoglycoside 2'-N-acetyltransferase activity
B	0016740	molecular_function	transferase activity
B	0016746	molecular_function	acyltransferase activity
B	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
B	0046677	biological_process	response to antibiotic
B	0047921	molecular_function	aminoglycoside 2'-N-acetyltransferase activity
C	0016740	molecular_function	transferase activity
C	0016746	molecular_function	acyltransferase activity
C	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
C	0046677	biological_process	response to antibiotic
C	0047921	molecular_function	aminoglycoside 2'-N-acetyltransferase activity
D	0016740	molecular_function	transferase activity
D	0016746	molecular_function	acyltransferase activity
D	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
D	0046677	biological_process	response to antibiotic
D	0047921	molecular_function	aminoglycoside 2'-N-acetyltransferase activity
E	0016740	molecular_function	transferase activity
E	0016746	molecular_function	acyltransferase activity
E	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
E	0046677	biological_process	response to antibiotic
E	0047921	molecular_function	aminoglycoside 2'-N-acetyltransferase activity
F	0016740	molecular_function	transferase activity
F	0016746	molecular_function	acyltransferase activity
F	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
F	0046677	biological_process	response to antibiotic
F	0047921	molecular_function	aminoglycoside 2'-N-acetyltransferase activity
G	0016740	molecular_function	transferase activity
G	0016746	molecular_function	acyltransferase activity
G	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
G	0046677	biological_process	response to antibiotic
G	0047921	molecular_function	aminoglycoside 2'-N-acetyltransferase activity
H	0016740	molecular_function	transferase activity
H	0016746	molecular_function	acyltransferase activity
H	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
H	0046677	biological_process	response to antibiotic
H	0047921	molecular_function	aminoglycoside 2'-N-acetyltransferase activity
I	0016740	molecular_function	transferase activity
I	0016746	molecular_function	acyltransferase activity
I	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
I	0046677	biological_process	response to antibiotic
I	0047921	molecular_function	aminoglycoside 2'-N-acetyltransferase activity
J	0016740	molecular_function	transferase activity
J	0016746	molecular_function	acyltransferase activity
J	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
J	0046677	biological_process	response to antibiotic
J	0047921	molecular_function	aminoglycoside 2'-N-acetyltransferase activity
K	0016740	molecular_function	transferase activity
K	0016746	molecular_function	acyltransferase activity
K	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
K	0046677	biological_process	response to antibiotic
K	0047921	molecular_function	aminoglycoside 2'-N-acetyltransferase activity
L	0016740	molecular_function	transferase activity
L	0016746	molecular_function	acyltransferase activity
L	0016747	molecular_function	acyltransferase activity, transferring groups other than amino-acyl groups
L	0046677	biological_process	response to antibiotic
L	0047921	molecular_function	aminoglycoside 2'-N-acetyltransferase activity

Functional Information from PDB Data

site_id	AC1
Number of Residues	13
Details	binding site for residue 8MM A 201

Chain	Residue
A	ASP37
A	GLU149
A	ASP176
A	TRP178
A	HOH334
A	GLU79
A	ALA80
A	MET81
A	SER114
A	SER116
A	ASP117
A	TYR123
A	GLU148

site_id	AC2
Number of Residues	8
Details	binding site for residue TAR A 202

Chain	Residue
A	ARG88
A	GLN90
A	GLY91
A	ILE92
A	GLY93
A	ARG94
A	LEU122
A	HOH312

site_id	AC3
Number of Residues	12
Details	binding site for residue 8MM B 400

Chain	Residue
B	ASP32
B	ASP37
B	ALA80
B	MET81
B	SER114
B	ALA115
B	SER116
B	ASP117
B	TYR123
B	GLU149
B	TRP178
B	HOH512

site_id	AC4
Number of Residues	16
Details	binding site for residue ACO C 201

Chain	Residue
C	ALA80
C	MET81
C	VAL82
C	VAL83
C	ARG88
C	ARG89
C	GLN90
C	GLY91
C	GLY93
C	ARG94
C	SER114
C	TYR123
C	HOH302
C	HOH303
G	TYR22
G	ASP23

site_id	AC5
Number of Residues	4
Details	binding site for residue GOL C 202

Chain	Residue
C	ARG6
C	GLU17
C	PHE50
C	GLN53

site_id	AC6
Number of Residues	11
Details	binding site for residue 8MM D 201

Chain	Residue
D	PHE33
D	ASP37
D	ALA80
D	MET81
D	SER114
D	SER116
D	TYR123
D	GLU148
D	GLU149
D	TRP178
D	HOH319

site_id	AC7
Number of Residues	7
Details	binding site for residue TAR D 202

Chain	Residue
D	VAL83
D	ARG88
D	ARG89
D	GLN90
D	GLY91
D	GLY93
D	ARG94

site_id	AC8
Number of Residues	13
Details	binding site for residue 8MM E 201

Chain	Residue
E	ASP37
E	ALA80
E	MET81
E	SER114
E	SER116
E	ASP117
E	TYR123
E	GLU148
E	GLU149
E	ASP176
E	TRP178
E	COA202
E	HOH323

site_id	AC9
Number of Residues	20
Details	binding site for residue COA E 202

Chain	Residue
E	8MM201
E	HOH302
E	HOH317
E	HOH321
J	GLN129
B	TYR22
B	ASP23
E	PHE29
E	MET81
E	VAL82
E	VAL83
E	ARG88
E	ARG89
E	GLN90
E	GLY91
E	GLY93
E	ARG94
E	SER116
E	LYS121
E	TYR123

site_id	AD1
Number of Residues	10
Details	binding site for residue 8MM F 201

Chain	Residue
F	ASP32
F	ASP37
F	MET81
F	SER114
F	SER116
F	GLU148
F	GLU149
F	ASP176
F	TRP178
F	HOH312

site_id	AD2
Number of Residues	10
Details	binding site for residue TAR F 202

Chain	Residue
F	VAL83
F	TYR87
F	ARG88
F	ARG89
F	GLN90
F	GLY91
F	ILE92
F	GLY93
F	ARG94
F	LEU122

site_id	AD3
Number of Residues	11
Details	binding site for residue 8MM G 400

Chain	Residue
G	ASP32
G	ASP37
G	ALA80
G	MET81
G	SER114
G	SER116
G	TYR123
G	GLU149
G	ASP176
G	TRP178
G	HOH502

site_id	AD4
Number of Residues	9
Details	binding site for residue 8MM H 201

Chain	Residue
H	ASP37
H	VAL78
H	ALA80
H	MET81
H	SER114
H	SER116
H	TYR123
H	GLU149
H	TRP178

site_id	AD5
Number of Residues	4
Details	binding site for residue TAR H 202

Chain	Residue
H	GLY91
H	ILE92
H	GLY93
H	LEU122

site_id	AD6
Number of Residues	14
Details	binding site for residue 8MM I 201

Chain	Residue
I	ASP37
I	VAL78
I	GLU79
I	ALA80
I	MET81
I	SER114
I	SER116
I	ASP117
I	TYR123
I	GLU149
I	ASP176
I	TRP178
I	COA202
I	HOH322

site_id	AD7
Number of Residues	15
Details	binding site for residue COA I 202

Chain	Residue
I	GLY28
I	PHE29
I	MET81
I	VAL82
I	VAL83
I	ARG88
I	ARG89
I	GLY91
I	GLY93
I	ARG94
I	SER116
I	LYS121
I	TYR123
I	8MM201
I	HOH302

site_id	AD8
Number of Residues	4
Details	binding site for residue GOL I 203

Chain	Residue
I	LEU98
I	ASN101
I	VAL126
I	HOH301

site_id	AD9
Number of Residues	5
Details	binding site for residue TAR J 201

Chain	Residue
J	VAL83
J	GLY91
J	GLY93
J	ARG94
J	LEU122

site_id	AE1
Number of Residues	14
Details	binding site for residue 8MM K 201

Chain	Residue
K	ASP32
K	ASP37
K	VAL78
K	GLU79
K	ALA80
K	MET81
K	SER114
K	SER116
K	ASP117
K	TYR123
K	GLU149
K	TRP178
K	HOH306
K	HOH324

site_id	AE2
Number of Residues	5
Details	binding site for residue GOL K 202

Chain	Residue
I	LYS134
K	ARG94
K	GLN95
K	LEU98
K	HOH312

site_id	AE3
Number of Residues	8
Details	binding site for residue 8MM L 201

Chain	Residue
L	ASP37
L	ALA80
L	MET81
L	SER114
L	ALA115
L	GLU149
L	TRP178
L	COA202

site_id	AE4
Number of Residues	17
Details	binding site for residue COA L 202

Chain	Residue
L	GLY28
L	PHE29
L	MET81
L	VAL82
L	VAL83
L	ARG88
L	ARG89
L	GLN90
L	GLY91
L	ILE92
L	GLY93
L	ARG94
L	ALA115
L	SER116
L	LYS121
L	TYR123
L	8MM201

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1793
Details	Domain: {"description":"N-acetyltransferase","evidences":[{"source":"PROSITE-ProRule","id":"PRU00532","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	131
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P9WQG9","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)