5TEY

Human METTL3-METTL14 complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000398	biological_process	mRNA splicing, via spliceosome
A	0001510	biological_process	RNA methylation
A	0001734	molecular_function	mRNA m(6)A methyltransferase activity
A	0003723	molecular_function	RNA binding
A	0003729	molecular_function	mRNA binding
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005794	cellular_component	Golgi apparatus
A	0005829	cellular_component	cytosol
A	0006397	biological_process	mRNA processing
A	0006974	biological_process	DNA damage response
A	0007283	biological_process	spermatogenesis
A	0007623	biological_process	circadian rhythm
A	0008168	molecular_function	methyltransferase activity
A	0008173	molecular_function	RNA methyltransferase activity
A	0008757	molecular_function	S-adenosylmethionine-dependent methyltransferase activity
A	0009048	biological_process	dosage compensation by inactivation of X chromosome
A	0016556	biological_process	mRNA modification
A	0016604	cellular_component	nuclear body
A	0016607	cellular_component	nuclear speck
A	0019827	biological_process	stem cell population maintenance
A	0021861	biological_process	forebrain radial glial cell differentiation
A	0030154	biological_process	cell differentiation
A	0031053	biological_process	primary miRNA processing
A	0032259	biological_process	methylation
A	0034644	biological_process	cellular response to UV
A	0036396	cellular_component	RNA N6-methyladenosine methyltransferase complex
A	0042063	biological_process	gliogenesis
A	0045087	biological_process	innate immune response
A	0045580	biological_process	regulation of T cell differentiation
A	0045727	biological_process	positive regulation of translation
A	0045746	biological_process	negative regulation of Notch signaling pathway
A	0046982	molecular_function	protein heterodimerization activity
A	0048477	biological_process	oogenesis
A	0048511	biological_process	rhythmic process
A	0051445	biological_process	regulation of meiotic cell cycle
A	0060339	biological_process	negative regulation of type I interferon-mediated signaling pathway
A	0061157	biological_process	mRNA destabilization
A	0098508	biological_process	endothelial to hematopoietic transition
A	0140640	molecular_function	catalytic activity, acting on a nucleic acid
A	1902036	biological_process	regulation of hematopoietic stem cell differentiation
A	1903679	biological_process	positive regulation of cap-independent translational initiation
A	1904047	molecular_function	S-adenosyl-L-methionine binding
A	1990204	cellular_component	oxidoreductase complex

Functional Information from PDB Data

site_id	AC1
Number of Residues	20
Details	binding site for residue SAH A 601

Chain	Residue
A	CYS376
A	HIS512
A	LYS513
A	PHE534
A	ARG536
A	HIS538
A	ASN539
A	ASN549
A	GLN550
A	HOH729
A	HOH736
A	ASP377
A	HOH760
A	ILE378
A	ASP395
A	PRO397
A	TYR406
A	GLY407
A	LEU409
A	SER511

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site_id	AC2
Number of Residues	6
Details	binding site for residue MG A 602

Chain	Residue
A	GLU506
A	HOH705
A	HOH726
A	HOH741
A	HOH776
B	ASN117

---

site_id	AC3
Number of Residues	6
Details	binding site for residue EDO A 603

Chain	Residue
A	ASN445
A	ARG451
A	HOH773
B	ASP242
B	ASN368
B	HOH559

---

site_id	AC4
Number of Residues	5
Details	binding site for residue BME B 401

Chain	Residue
A	HIS474
A	TRP475
A	LEU476
B	CYS256
B	GLU257

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	5
Details	SITE: Interaction with METTL3 => ECO:0000269|PubMed:27281194, ECO:0007744|PDB:5IL0, ECO:0007744|PDB:5IL1, ECO:0007744|PDB:5IL2

Chain	Residue	Details
B	TYR146
B	ASP242
B	ARG245
B	ARG298
B	SER399

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:27281194, ECO:0000269|PubMed:29348140, ECO:0007744|PDB:5IL0, ECO:0007744|PDB:5IL1, ECO:0007744|PDB:5IL2, ECO:0007744|PubMed:24275569

Chain	Residue	Details
B	SER399
A	LYS513

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	SITE: Interaction with METTL14 => ECO:0000269|PubMed:27281194, ECO:0007744|PDB:5IL1, ECO:0007744|PDB:5IL2

Chain	Residue	Details
A	GLU438
A	ARG441

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: Phosphoserine; alternate => ECO:0000269|PubMed:29348140

Chain	Residue	Details
A	SER2

---

site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:29348140, ECO:0007744|PubMed:16964243, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER43

---

site_id	SWS_FT_FI6
Number of Residues	3
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:29348140

Chain	Residue	Details
A	SER48
A	SER50
A	SER350

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:29348140, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER219

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	MOD_RES: Phosphoserine => ECO:0000269|PubMed:29348140, ECO:0007744|PubMed:18669648

Chain	Residue	Details
A	SER243

---

site_id	SWS_FT_FI9
Number of Residues	1
Details	MOD_RES: Phosphothreonine => ECO:0000269|PubMed:29348140, ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	THR348

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site_id	SWS_FT_FI10
Number of Residues	5
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1) => ECO:0000269|PubMed:29506078

Chain	Residue	Details
A	LYS177
A	LYS211
A	LYS212
A	LYS215

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