5TEY
Human METTL3-METTL14 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-01-29 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97957 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 63.883, 63.883, 225.709 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 50.000 - 1.800 |
R-factor | 0.1984 |
Rwork | 0.198 |
R-free | 0.22410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5il0 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.409 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER (2.6.1) |
Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
Rmerge | 0.117 | 0.059 | 0.868 |
Number of reflections | 50717 | ||
<I/σ(I)> | 8 | ||
Completeness [%] | 99.9 | 100 | 100 |
Redundancy | 7.7 | 7.6 | 6.4 |
CC(1/2) | 0.998 | 0.615 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 23% PEG 4K, 0.2 M MgCl2, 0.1 M Tris pH8.5 |