5TEY

Human METTL3-METTL14 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-01-29
Detector	RAYONIX MX-300
Wavelength(s)	0.97957
Spacegroup name	P 32 2 1
Unit cell lengths	63.883, 63.883, 225.709
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 1.800
R-factor	0.1984
Rwork	0.198
R-free	0.22410
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5il0
RMSD bond length	0.010
RMSD bond angle	1.409
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER (2.6.1)
Refinement software	REFMAC (5.8.0155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.830
High resolution limit [Å]	1.800	4.880	1.800
Rmerge	0.117	0.059	0.868
Number of reflections	50717
<I/σ(I)>	8
Completeness [%]	99.9	100	100
Redundancy	7.7	7.6	6.4
CC(1/2)		0.998	0.615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	23% PEG 4K, 0.2 M MgCl2, 0.1 M Tris pH8.5