5TBK

Crystal structure of human importin a3 bound to RCC1

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0001673	cellular_component	male germ cell nucleus
A	0005515	molecular_function	protein binding
A	0005634	cellular_component	nucleus
A	0005643	cellular_component	nuclear pore
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0006606	biological_process	protein import into nucleus
A	0006607	biological_process	NLS-bearing protein import into nucleus
A	0008139	molecular_function	nuclear localization sequence binding
A	0015031	biological_process	protein transport
A	0031965	cellular_component	nuclear membrane
A	0042542	biological_process	response to hydrogen peroxide
A	0042564	cellular_component	NLS-dependent protein nuclear import complex
A	0061608	molecular_function	nuclear import signal receptor activity
B	0001673	cellular_component	male germ cell nucleus
B	0005515	molecular_function	protein binding
B	0005634	cellular_component	nucleus
B	0005643	cellular_component	nuclear pore
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0006606	biological_process	protein import into nucleus
B	0006607	biological_process	NLS-bearing protein import into nucleus
B	0008139	molecular_function	nuclear localization sequence binding
B	0015031	biological_process	protein transport
B	0031965	cellular_component	nuclear membrane
B	0042542	biological_process	response to hydrogen peroxide
B	0042564	cellular_component	NLS-dependent protein nuclear import complex
B	0061608	molecular_function	nuclear import signal receptor activity
C	0001673	cellular_component	male germ cell nucleus
C	0005515	molecular_function	protein binding
C	0005634	cellular_component	nucleus
C	0005643	cellular_component	nuclear pore
C	0005654	cellular_component	nucleoplasm
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0006606	biological_process	protein import into nucleus
C	0006607	biological_process	NLS-bearing protein import into nucleus
C	0008139	molecular_function	nuclear localization sequence binding
C	0015031	biological_process	protein transport
C	0031965	cellular_component	nuclear membrane
C	0042542	biological_process	response to hydrogen peroxide
C	0042564	cellular_component	NLS-dependent protein nuclear import complex
C	0061608	molecular_function	nuclear import signal receptor activity
D	0001673	cellular_component	male germ cell nucleus
D	0005515	molecular_function	protein binding
D	0005634	cellular_component	nucleus
D	0005643	cellular_component	nuclear pore
D	0005654	cellular_component	nucleoplasm
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0006606	biological_process	protein import into nucleus
D	0006607	biological_process	NLS-bearing protein import into nucleus
D	0008139	molecular_function	nuclear localization sequence binding
D	0015031	biological_process	protein transport
D	0031965	cellular_component	nuclear membrane
D	0042542	biological_process	response to hydrogen peroxide
D	0042564	cellular_component	NLS-dependent protein nuclear import complex
D	0061608	molecular_function	nuclear import signal receptor activity
E	0001673	cellular_component	male germ cell nucleus
E	0005515	molecular_function	protein binding
E	0005634	cellular_component	nucleus
E	0005643	cellular_component	nuclear pore
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0006606	biological_process	protein import into nucleus
E	0006607	biological_process	NLS-bearing protein import into nucleus
E	0008139	molecular_function	nuclear localization sequence binding
E	0015031	biological_process	protein transport
E	0031965	cellular_component	nuclear membrane
E	0042542	biological_process	response to hydrogen peroxide
E	0042564	cellular_component	NLS-dependent protein nuclear import complex
E	0061608	molecular_function	nuclear import signal receptor activity
F	0001673	cellular_component	male germ cell nucleus
F	0005515	molecular_function	protein binding
F	0005634	cellular_component	nucleus
F	0005643	cellular_component	nuclear pore
F	0005654	cellular_component	nucleoplasm
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0006606	biological_process	protein import into nucleus
F	0006607	biological_process	NLS-bearing protein import into nucleus
F	0008139	molecular_function	nuclear localization sequence binding
F	0015031	biological_process	protein transport
F	0031965	cellular_component	nuclear membrane
F	0042542	biological_process	response to hydrogen peroxide
F	0042564	cellular_component	NLS-dependent protein nuclear import complex
F	0061608	molecular_function	nuclear import signal receptor activity
G	0001673	cellular_component	male germ cell nucleus
G	0005515	molecular_function	protein binding
G	0005634	cellular_component	nucleus
G	0005643	cellular_component	nuclear pore
G	0005654	cellular_component	nucleoplasm
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0006606	biological_process	protein import into nucleus
G	0006607	biological_process	NLS-bearing protein import into nucleus
G	0008139	molecular_function	nuclear localization sequence binding
G	0015031	biological_process	protein transport
G	0031965	cellular_component	nuclear membrane
G	0042542	biological_process	response to hydrogen peroxide
G	0042564	cellular_component	NLS-dependent protein nuclear import complex
G	0061608	molecular_function	nuclear import signal receptor activity
H	0001673	cellular_component	male germ cell nucleus
H	0005515	molecular_function	protein binding
H	0005634	cellular_component	nucleus
H	0005643	cellular_component	nuclear pore
H	0005654	cellular_component	nucleoplasm
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0006606	biological_process	protein import into nucleus
H	0006607	biological_process	NLS-bearing protein import into nucleus
H	0008139	molecular_function	nuclear localization sequence binding
H	0015031	biological_process	protein transport
H	0031965	cellular_component	nuclear membrane
H	0042542	biological_process	response to hydrogen peroxide
H	0042564	cellular_component	NLS-dependent protein nuclear import complex
H	0061608	molecular_function	nuclear import signal receptor activity
I	0000082	biological_process	G1/S transition of mitotic cell cycle
I	0000785	cellular_component	chromatin
I	0000794	cellular_component	condensed nuclear chromosome
I	0003677	molecular_function	DNA binding
I	0003682	molecular_function	chromatin binding
I	0005085	molecular_function	guanyl-nucleotide exchange factor activity
I	0005515	molecular_function	protein binding
I	0005634	cellular_component	nucleus
I	0005654	cellular_component	nucleoplasm
I	0005694	cellular_component	chromosome
I	0005737	cellular_component	cytoplasm
I	0007052	biological_process	mitotic spindle organization
I	0007059	biological_process	chromosome segregation
I	0007084	biological_process	mitotic nuclear membrane reassembly
I	0007088	biological_process	regulation of mitotic nuclear division
I	0007346	biological_process	regulation of mitotic cell cycle
I	0016032	biological_process	viral process
I	0031267	molecular_function	small GTPase binding
I	0031491	molecular_function	nucleosome binding
I	0031492	molecular_function	nucleosomal DNA binding
I	0032991	cellular_component	protein-containing complex
I	0042393	molecular_function	histone binding
I	0043199	molecular_function	sulfate binding
I	0046982	molecular_function	protein heterodimerization activity
I	0051225	biological_process	spindle assembly
I	0051301	biological_process	cell division
I	0090068	biological_process	positive regulation of cell cycle process
I	1901673	biological_process	regulation of mitotic spindle assembly
J	0000082	biological_process	G1/S transition of mitotic cell cycle
J	0000785	cellular_component	chromatin
J	0000794	cellular_component	condensed nuclear chromosome
J	0003677	molecular_function	DNA binding
J	0003682	molecular_function	chromatin binding
J	0005085	molecular_function	guanyl-nucleotide exchange factor activity
J	0005515	molecular_function	protein binding
J	0005634	cellular_component	nucleus
J	0005654	cellular_component	nucleoplasm
J	0005694	cellular_component	chromosome
J	0005737	cellular_component	cytoplasm
J	0007052	biological_process	mitotic spindle organization
J	0007059	biological_process	chromosome segregation
J	0007084	biological_process	mitotic nuclear membrane reassembly
J	0007088	biological_process	regulation of mitotic nuclear division
J	0007346	biological_process	regulation of mitotic cell cycle
J	0016032	biological_process	viral process
J	0031267	molecular_function	small GTPase binding
J	0031491	molecular_function	nucleosome binding
J	0031492	molecular_function	nucleosomal DNA binding
J	0032991	cellular_component	protein-containing complex
J	0042393	molecular_function	histone binding
J	0043199	molecular_function	sulfate binding
J	0046982	molecular_function	protein heterodimerization activity
J	0051225	biological_process	spindle assembly
J	0051301	biological_process	cell division
J	0090068	biological_process	positive regulation of cell cycle process
J	1901673	biological_process	regulation of mitotic spindle assembly
K	0000082	biological_process	G1/S transition of mitotic cell cycle
K	0000785	cellular_component	chromatin
K	0000794	cellular_component	condensed nuclear chromosome
K	0003677	molecular_function	DNA binding
K	0003682	molecular_function	chromatin binding
K	0005085	molecular_function	guanyl-nucleotide exchange factor activity
K	0005515	molecular_function	protein binding
K	0005634	cellular_component	nucleus
K	0005654	cellular_component	nucleoplasm
K	0005694	cellular_component	chromosome
K	0005737	cellular_component	cytoplasm
K	0007052	biological_process	mitotic spindle organization
K	0007059	biological_process	chromosome segregation
K	0007084	biological_process	mitotic nuclear membrane reassembly
K	0007088	biological_process	regulation of mitotic nuclear division
K	0007346	biological_process	regulation of mitotic cell cycle
K	0016032	biological_process	viral process
K	0031267	molecular_function	small GTPase binding
K	0031491	molecular_function	nucleosome binding
K	0031492	molecular_function	nucleosomal DNA binding
K	0032991	cellular_component	protein-containing complex
K	0042393	molecular_function	histone binding
K	0043199	molecular_function	sulfate binding
K	0046982	molecular_function	protein heterodimerization activity
K	0051225	biological_process	spindle assembly
K	0051301	biological_process	cell division
K	0090068	biological_process	positive regulation of cell cycle process
K	1901673	biological_process	regulation of mitotic spindle assembly
L	0000082	biological_process	G1/S transition of mitotic cell cycle
L	0000785	cellular_component	chromatin
L	0000794	cellular_component	condensed nuclear chromosome
L	0003677	molecular_function	DNA binding
L	0003682	molecular_function	chromatin binding
L	0005085	molecular_function	guanyl-nucleotide exchange factor activity
L	0005515	molecular_function	protein binding
L	0005634	cellular_component	nucleus
L	0005654	cellular_component	nucleoplasm
L	0005694	cellular_component	chromosome
L	0005737	cellular_component	cytoplasm
L	0007052	biological_process	mitotic spindle organization
L	0007059	biological_process	chromosome segregation
L	0007084	biological_process	mitotic nuclear membrane reassembly
L	0007088	biological_process	regulation of mitotic nuclear division
L	0007346	biological_process	regulation of mitotic cell cycle
L	0016032	biological_process	viral process
L	0031267	molecular_function	small GTPase binding
L	0031491	molecular_function	nucleosome binding
L	0031492	molecular_function	nucleosomal DNA binding
L	0032991	cellular_component	protein-containing complex
L	0042393	molecular_function	histone binding
L	0043199	molecular_function	sulfate binding
L	0046982	molecular_function	protein heterodimerization activity
L	0051225	biological_process	spindle assembly
L	0051301	biological_process	cell division
L	0090068	biological_process	positive regulation of cell cycle process
L	1901673	biological_process	regulation of mitotic spindle assembly
M	0000082	biological_process	G1/S transition of mitotic cell cycle
M	0000785	cellular_component	chromatin
M	0000794	cellular_component	condensed nuclear chromosome
M	0003677	molecular_function	DNA binding
M	0003682	molecular_function	chromatin binding
M	0005085	molecular_function	guanyl-nucleotide exchange factor activity
M	0005515	molecular_function	protein binding
M	0005634	cellular_component	nucleus
M	0005654	cellular_component	nucleoplasm
M	0005694	cellular_component	chromosome
M	0005737	cellular_component	cytoplasm
M	0007052	biological_process	mitotic spindle organization
M	0007059	biological_process	chromosome segregation
M	0007084	biological_process	mitotic nuclear membrane reassembly
M	0007088	biological_process	regulation of mitotic nuclear division
M	0007346	biological_process	regulation of mitotic cell cycle
M	0016032	biological_process	viral process
M	0031267	molecular_function	small GTPase binding
M	0031491	molecular_function	nucleosome binding
M	0031492	molecular_function	nucleosomal DNA binding
M	0032991	cellular_component	protein-containing complex
M	0042393	molecular_function	histone binding
M	0043199	molecular_function	sulfate binding
M	0046982	molecular_function	protein heterodimerization activity
M	0051225	biological_process	spindle assembly
M	0051301	biological_process	cell division
M	0090068	biological_process	positive regulation of cell cycle process
M	1901673	biological_process	regulation of mitotic spindle assembly
N	0000082	biological_process	G1/S transition of mitotic cell cycle
N	0000785	cellular_component	chromatin
N	0000794	cellular_component	condensed nuclear chromosome
N	0003677	molecular_function	DNA binding
N	0003682	molecular_function	chromatin binding
N	0005085	molecular_function	guanyl-nucleotide exchange factor activity
N	0005515	molecular_function	protein binding
N	0005634	cellular_component	nucleus
N	0005654	cellular_component	nucleoplasm
N	0005694	cellular_component	chromosome
N	0005737	cellular_component	cytoplasm
N	0007052	biological_process	mitotic spindle organization
N	0007059	biological_process	chromosome segregation
N	0007084	biological_process	mitotic nuclear membrane reassembly
N	0007088	biological_process	regulation of mitotic nuclear division
N	0007346	biological_process	regulation of mitotic cell cycle
N	0016032	biological_process	viral process
N	0031267	molecular_function	small GTPase binding
N	0031491	molecular_function	nucleosome binding
N	0031492	molecular_function	nucleosomal DNA binding
N	0032991	cellular_component	protein-containing complex
N	0042393	molecular_function	histone binding
N	0043199	molecular_function	sulfate binding
N	0046982	molecular_function	protein heterodimerization activity
N	0051225	biological_process	spindle assembly
N	0051301	biological_process	cell division
N	0090068	biological_process	positive regulation of cell cycle process
N	1901673	biological_process	regulation of mitotic spindle assembly
O	0000082	biological_process	G1/S transition of mitotic cell cycle
O	0000785	cellular_component	chromatin
O	0000794	cellular_component	condensed nuclear chromosome
O	0003677	molecular_function	DNA binding
O	0003682	molecular_function	chromatin binding
O	0005085	molecular_function	guanyl-nucleotide exchange factor activity
O	0005515	molecular_function	protein binding
O	0005634	cellular_component	nucleus
O	0005654	cellular_component	nucleoplasm
O	0005694	cellular_component	chromosome
O	0005737	cellular_component	cytoplasm
O	0007052	biological_process	mitotic spindle organization
O	0007059	biological_process	chromosome segregation
O	0007084	biological_process	mitotic nuclear membrane reassembly
O	0007088	biological_process	regulation of mitotic nuclear division
O	0007346	biological_process	regulation of mitotic cell cycle
O	0016032	biological_process	viral process
O	0031267	molecular_function	small GTPase binding
O	0031491	molecular_function	nucleosome binding
O	0031492	molecular_function	nucleosomal DNA binding
O	0032991	cellular_component	protein-containing complex
O	0042393	molecular_function	histone binding
O	0043199	molecular_function	sulfate binding
O	0046982	molecular_function	protein heterodimerization activity
O	0051225	biological_process	spindle assembly
O	0051301	biological_process	cell division
O	0090068	biological_process	positive regulation of cell cycle process
O	1901673	biological_process	regulation of mitotic spindle assembly
P	0000082	biological_process	G1/S transition of mitotic cell cycle
P	0000785	cellular_component	chromatin
P	0000794	cellular_component	condensed nuclear chromosome
P	0003677	molecular_function	DNA binding
P	0003682	molecular_function	chromatin binding
P	0005085	molecular_function	guanyl-nucleotide exchange factor activity
P	0005515	molecular_function	protein binding
P	0005634	cellular_component	nucleus
P	0005654	cellular_component	nucleoplasm
P	0005694	cellular_component	chromosome
P	0005737	cellular_component	cytoplasm
P	0007052	biological_process	mitotic spindle organization
P	0007059	biological_process	chromosome segregation
P	0007084	biological_process	mitotic nuclear membrane reassembly
P	0007088	biological_process	regulation of mitotic nuclear division
P	0007346	biological_process	regulation of mitotic cell cycle
P	0016032	biological_process	viral process
P	0031267	molecular_function	small GTPase binding
P	0031491	molecular_function	nucleosome binding
P	0031492	molecular_function	nucleosomal DNA binding
P	0032991	cellular_component	protein-containing complex
P	0042393	molecular_function	histone binding
P	0043199	molecular_function	sulfate binding
P	0046982	molecular_function	protein heterodimerization activity
P	0051225	biological_process	spindle assembly
P	0051301	biological_process	cell division
P	0090068	biological_process	positive regulation of cell cycle process
P	1901673	biological_process	regulation of mitotic spindle assembly

Functional Information from PROSITE/UniProt

site_id	PS00625
Number of Residues	12
Details	RCC1_1 Regulator of chromosome condensation (RCC1) signature 1. GcNDegALGRdT

Chain	Residue	Details
I	GLY92-THR103

---

site_id	PS00626
Number of Residues	11
Details	RCC1_2 Regulator of chromosome condensation (RCC1) signature 2. VSAGdSHTAAL

Chain	Residue	Details
I	VAL124-LEU134
I	VAL177-LEU187
I	PHE299-MET309
I	VAL404-LEU414

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	336
Details	Repeat: {"description":"ARM 2"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	352
Details	Repeat: {"description":"ARM 3"}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	304
Details	Repeat: {"description":"ARM 4"}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	352
Details	Repeat: {"description":"ARM 5"}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	312
Details	Repeat: {"description":"ARM 6"}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	328
Details	Repeat: {"description":"ARM 7"}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	312
Details	Repeat: {"description":"ARM 8"}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	336
Details	Repeat: {"description":"ARM 9"}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	304
Details	Repeat: {"description":"ARM 10; atypical"}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	736
Details	Region: {"description":"NLS binding site (major)","evidences":[{"source":"PubMed","id":"29042532","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	704
Details	Region: {"description":"NLS binding site (minor)","evidences":[{"source":"PubMed","id":"29042532","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	400
Details	Repeat: {"description":"RCC1 1"}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	408
Details	Repeat: {"description":"RCC1 2"}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	408
Details	Repeat: {"description":"RCC1 3"}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	528
Details	Repeat: {"description":"RCC1 4"}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	424
Details	Repeat: {"description":"RCC1 5"}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	400
Details	Repeat: {"description":"RCC1 6"}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	424
Details	Repeat: {"description":"RCC1 7"}

Chain

Residue

Details

---

site_id	SWS_FT_FI19
Number of Residues	160
Details	Motif: {"description":"Bipartite nuclear localization signal","evidences":[{"source":"PubMed","id":"29042532","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI20
Number of Residues	80
Details	Compositional bias: {"description":"Basic residues","evidences":[{"source":"SAM","id":"MobiDB-lite","evidenceCode":"ECO:0000256"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI21
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"17081983","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"21406692","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"24275569","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---