5TBK
Crystal structure of human importin a3 bound to RCC1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 |
| Unit cell lengths | 127.403, 162.494, 161.592 |
| Unit cell angles | 75.65, 85.63, 72.17 |
Refinement procedure
| Resolution | 19.988 - 3.450 |
| R-factor | 0.2784 |
| Rwork | 0.278 |
| R-free | 0.29600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4UAE and 1A12 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.684 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1-2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.530 |
| High resolution limit [Å] | 3.450 | 3.450 |
| Rmerge | 0.650 | |
| Number of reflections | 935054 | |
| <I/σ(I)> | 12.2 | 1.8 |
| Completeness [%] | 90.4 | 71.5 |
| Redundancy | 2 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1 M sodium cacodylate buffer (pH 6.5), 0.2M Calcium acetate, 8% PEG 8000 and galactose 3% |
