5TB5

Crystal structure of full-length farnesylated and methylated KRAS4b in complex with PDE-delta (crystal form I - with partially disordered hypervariable region)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000139	cellular_component	Golgi membrane
A	0000165	biological_process	MAPK cascade
A	0000166	molecular_function	nucleotide binding
A	0001889	biological_process	liver development
A	0003924	molecular_function	GTPase activity
A	0003925	molecular_function	G protein activity
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005737	cellular_component	cytoplasm
A	0005741	cellular_component	mitochondrial outer membrane
A	0005789	cellular_component	endoplasmic reticulum membrane
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0005925	cellular_component	focal adhesion
A	0007165	biological_process	signal transduction
A	0007265	biological_process	Ras protein signal transduction
A	0007565	biological_process	female pregnancy
A	0008283	biological_process	cell population proliferation
A	0008542	biological_process	visual learning
A	0009629	biological_process	response to gravity
A	0009898	cellular_component	cytoplasmic side of plasma membrane
A	0010467	biological_process	gene expression
A	0010628	biological_process	positive regulation of gene expression
A	0012505	cellular_component	endomembrane system
A	0014009	biological_process	glial cell proliferation
A	0016020	cellular_component	membrane
A	0016601	biological_process	Rac protein signal transduction
A	0016787	molecular_function	hydrolase activity
A	0019002	molecular_function	GMP binding
A	0019003	molecular_function	GDP binding
A	0019221	biological_process	cytokine-mediated signaling pathway
A	0021897	biological_process	forebrain astrocyte development
A	0030036	biological_process	actin cytoskeleton organization
A	0030275	molecular_function	LRR domain binding
A	0030857	biological_process	negative regulation of epithelial cell differentiation
A	0032228	biological_process	regulation of synaptic transmission, GABAergic
A	0035022	biological_process	positive regulation of Rac protein signal transduction
A	0035900	biological_process	response to isolation stress
A	0035914	biological_process	skeletal muscle cell differentiation
A	0043495	molecular_function	protein-membrane adaptor activity
A	0043524	biological_process	negative regulation of neuron apoptotic process
A	0044877	molecular_function	protein-containing complex binding
A	0048169	biological_process	regulation of long-term neuronal synaptic plasticity
A	0048873	biological_process	homeostasis of number of cells within a tissue
A	0051146	biological_process	striated muscle cell differentiation
A	0051384	biological_process	response to glucocorticoid
A	0051385	biological_process	response to mineralocorticoid
A	0051402	biological_process	neuron apoptotic process
A	0051450	biological_process	myoblast proliferation
A	0060038	biological_process	cardiac muscle cell proliferation
A	0060252	biological_process	positive regulation of glial cell proliferation
A	0060441	biological_process	epithelial tube branching involved in lung morphogenesis
A	0060509	biological_process	type I pneumocyte differentiation
A	2000774	biological_process	positive regulation of cellular senescence
B	0005095	molecular_function	GTPase inhibitor activity
B	0005515	molecular_function	protein binding
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005929	cellular_component	cilium
B	0007601	biological_process	visual perception
B	0030659	cellular_component	cytoplasmic vesicle membrane
B	0031267	molecular_function	small GTPase binding
B	0031410	cellular_component	cytoplasmic vesicle
B	0050953	biological_process	sensory perception of light stimulus
C	0000139	cellular_component	Golgi membrane
C	0000165	biological_process	MAPK cascade
C	0000166	molecular_function	nucleotide binding
C	0001889	biological_process	liver development
C	0003924	molecular_function	GTPase activity
C	0003925	molecular_function	G protein activity
C	0005515	molecular_function	protein binding
C	0005525	molecular_function	GTP binding
C	0005737	cellular_component	cytoplasm
C	0005741	cellular_component	mitochondrial outer membrane
C	0005789	cellular_component	endoplasmic reticulum membrane
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0005925	cellular_component	focal adhesion
C	0007165	biological_process	signal transduction
C	0007265	biological_process	Ras protein signal transduction
C	0007565	biological_process	female pregnancy
C	0008283	biological_process	cell population proliferation
C	0008542	biological_process	visual learning
C	0009629	biological_process	response to gravity
C	0009898	cellular_component	cytoplasmic side of plasma membrane
C	0010467	biological_process	gene expression
C	0010628	biological_process	positive regulation of gene expression
C	0012505	cellular_component	endomembrane system
C	0014009	biological_process	glial cell proliferation
C	0016020	cellular_component	membrane
C	0016601	biological_process	Rac protein signal transduction
C	0016787	molecular_function	hydrolase activity
C	0019002	molecular_function	GMP binding
C	0019003	molecular_function	GDP binding
C	0019221	biological_process	cytokine-mediated signaling pathway
C	0021897	biological_process	forebrain astrocyte development
C	0030036	biological_process	actin cytoskeleton organization
C	0030275	molecular_function	LRR domain binding
C	0030857	biological_process	negative regulation of epithelial cell differentiation
C	0032228	biological_process	regulation of synaptic transmission, GABAergic
C	0035022	biological_process	positive regulation of Rac protein signal transduction
C	0035900	biological_process	response to isolation stress
C	0035914	biological_process	skeletal muscle cell differentiation
C	0043495	molecular_function	protein-membrane adaptor activity
C	0043524	biological_process	negative regulation of neuron apoptotic process
C	0044877	molecular_function	protein-containing complex binding
C	0048169	biological_process	regulation of long-term neuronal synaptic plasticity
C	0048873	biological_process	homeostasis of number of cells within a tissue
C	0051146	biological_process	striated muscle cell differentiation
C	0051384	biological_process	response to glucocorticoid
C	0051385	biological_process	response to mineralocorticoid
C	0051402	biological_process	neuron apoptotic process
C	0051450	biological_process	myoblast proliferation
C	0060038	biological_process	cardiac muscle cell proliferation
C	0060252	biological_process	positive regulation of glial cell proliferation
C	0060441	biological_process	epithelial tube branching involved in lung morphogenesis
C	0060509	biological_process	type I pneumocyte differentiation
C	2000774	biological_process	positive regulation of cellular senescence
D	0005095	molecular_function	GTPase inhibitor activity
D	0005515	molecular_function	protein binding
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005929	cellular_component	cilium
D	0007601	biological_process	visual perception
D	0030659	cellular_component	cytoplasmic vesicle membrane
D	0031267	molecular_function	small GTPase binding
D	0031410	cellular_component	cytoplasmic vesicle
D	0050953	biological_process	sensory perception of light stimulus

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	binding site for residue FAR A 201

Chain	Residue
A	LYS184
A	CMT185
B	SER39
B	LEU63
B	GLN78
B	THR131
B	PHE133

---

site_id	AC2
Number of Residues	17
Details	binding site for residue GDP A 202

Chain	Residue
A	GLY15
A	LYS16
A	SER17
A	ALA18
A	PHE28
A	ASN116
A	LYS117
A	ASP119
A	LEU120
A	SER145
A	ALA146
A	LYS147
A	HOH319
A	HOH345
A	HOH348
A	GLY13
A	VAL14

---

site_id	AC3
Number of Residues	5
Details	binding site for residue EDO B 201

Chain	Residue
B	ARG61
B	LEU63
B	PHE92
B	TRP105
B	SER107

---

site_id	AC4
Number of Residues	9
Details	binding site for residue FAR C 201

Chain	Residue
C	LYS184
C	CMT185
D	MET20
D	LEU22
D	SER39
D	LEU76
D	GLN78
D	THR131
D	PHE133

---

site_id	AC5
Number of Residues	18
Details	binding site for residue GDP C 202

Chain	Residue
C	ALA11
C	GLY13
C	VAL14
C	GLY15
C	LYS16
C	SER17
C	ALA18
C	PHE28
C	ASP30
C	ALA59
C	ASN116
C	LYS117
C	ASP119
C	LEU120
C	SER145
C	ALA146
C	LYS147
C	HOH303

---

site_id	AC6
Number of Residues	5
Details	binding site for residue EDO D 201

Chain	Residue
D	ARG61
D	LEU63
D	PHE92
D	TRP105
D	SER107

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	24
Details	Binding site: {"evidences":[{"source":"PubMed","id":"22431598","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"22566140","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"34380736","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"35522713","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	2
Details	Modified residue: {"description":"N-acetylthreonine; in GTPase KRas, N-terminally processed","evidences":[{"source":"Reference","evidenceCode":"ECO:0000269","citation":{"citationType":"submission","publicationDate":"FEB-2008","submissionDatabase":"UniProtKB","authors":["Bienvenut W.V.","Calvo F.","Kolch W."]}}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"22711838","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"PubMed","id":"30442762","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	12
Details	Region: {"description":"Required for association with membranes","evidences":[{"source":"PubMed","id":"9712853","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---