5OVW
Nanobody-bound BtuF, the vitamin B12 binding protein in Escherichia coli
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0015889 | biological_process | cobalamin transport |
| A | 0031419 | molecular_function | cobalamin binding |
| A | 0042597 | cellular_component | periplasmic space |
| B | 0015889 | biological_process | cobalamin transport |
| B | 0031419 | molecular_function | cobalamin binding |
| B | 0042597 | cellular_component | periplasmic space |
| C | 0015889 | biological_process | cobalamin transport |
| C | 0031419 | molecular_function | cobalamin binding |
| C | 0042597 | cellular_component | periplasmic space |
| D | 0015889 | biological_process | cobalamin transport |
| D | 0031419 | molecular_function | cobalamin binding |
| D | 0042597 | cellular_component | periplasmic space |
| E | 0015889 | biological_process | cobalamin transport |
| E | 0031419 | molecular_function | cobalamin binding |
| E | 0042597 | cellular_component | periplasmic space |
| F | 0015889 | biological_process | cobalamin transport |
| F | 0031419 | molecular_function | cobalamin binding |
| F | 0042597 | cellular_component | periplasmic space |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 6 |
| Details | binding site for residue GOL B 301 |
| Chain | Residue |
| B | PHE168 |
| B | TRP196 |
| B | GLN198 |
| I | TYR54 |
| I | PHE125 |
| I | TYR136 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | binding site for residue GOL D 301 |
| Chain | Residue |
| D | HOH401 |
| J | TYR54 |
| J | TYR136 |
| D | PHE168 |
| D | TRP196 |
| D | GLN198 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue GOL E 301 |
| Chain | Residue |
| E | PHE168 |
| E | TRP196 |
| E | GLN198 |
| K | TYR54 |
| K | PHE125 |
| K | TYR136 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | binding site for residue GOL F 301 |
| Chain | Residue |
| F | PHE168 |
| F | TRP196 |
| F | GLN198 |
| L | TYR54 |
| L | TYR136 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | binding site for residue GOL H 201 |
| Chain | Residue |
| A | TRP196 |
| A | GLN198 |
| H | TYR54 |
| H | TYR136 |
| H | HOH306 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01000, ECO:0000269|PubMed:12468528, ECO:0007744|PDB:1N4A |
| Chain | Residue | Details |
| A | TYR50 | |
| B | TYR50 | |
| C | TYR50 | |
| D | TYR50 | |
| E | TYR50 | |
| F | TYR50 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_01000, ECO:0000269|PubMed:12468528, ECO:0000269|PubMed:12475936, ECO:0007744|PDB:1N2Z, ECO:0007744|PDB:1N4A |
| Chain | Residue | Details |
| A | ASP242 | |
| B | ASP242 | |
| C | ASP242 | |
| D | ASP242 | |
| E | ASP242 | |
| F | ASP242 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 12 |
| Details | SITE: Important for BtuC binding => ECO:0000255|HAMAP-Rule:MF_01000, ECO:0000305|PubMed:12475936 |
| Chain | Residue | Details |
| A | GLU72 | |
| E | GLU202 | |
| F | GLU72 | |
| F | GLU202 | |
| A | GLU202 | |
| B | GLU72 | |
| B | GLU202 | |
| C | GLU72 | |
| C | GLU202 | |
| D | GLU72 | |
| D | GLU202 | |
| E | GLU72 |
