5OVW
Nanobody-bound BtuF, the vitamin B12 binding protein in Escherichia coli
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-09-30 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.99984 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 83.800, 141.000, 216.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.996 - 2.653 |
R-factor | 0.2251 |
Rwork | 0.223 |
R-free | 0.25940 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.003 |
RMSD bond angle | 0.558 |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 19.996 |
High resolution limit [Å] | 2.653 |
Number of reflections | 63910 |
<I/σ(I)> | 1.36 |
Completeness [%] | 85.3 |
Redundancy | 6.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | PEG4000, Tris-HCl, Magnesium chloride |