5O2S

Human KRAS in complex with darpin K27

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003924	molecular_function	GTPase activity
A	0005525	molecular_function	GTP binding
A	0007165	biological_process	signal transduction
A	0016020	cellular_component	membrane
C	0003924	molecular_function	GTPase activity
C	0005525	molecular_function	GTP binding
C	0007165	biological_process	signal transduction
C	0016020	cellular_component	membrane
E	0003924	molecular_function	GTPase activity
E	0005525	molecular_function	GTP binding
E	0007165	biological_process	signal transduction
E	0016020	cellular_component	membrane
G	0003924	molecular_function	GTPase activity
G	0005525	molecular_function	GTP binding
G	0007165	biological_process	signal transduction
G	0016020	cellular_component	membrane

Functional Information from PDB Data

site_id	AC1
Number of Residues	21
Details	binding site for residue GDP A 201

Chain	Residue
A	GLY13
A	TYR32
A	ASN116
A	LYS117
A	ASP119
A	LEU120
A	SER145
A	ALA146
A	LYS147
A	MG202
A	HOH301
A	GLY15
A	HOH302
A	HOH304
A	LYS16
A	SER17
A	ALA18
A	PHE28
A	VAL29
A	ASP30
A	GLU31

---

site_id	AC2
Number of Residues	6
Details	binding site for residue MG A 202

Chain	Residue
A	SER17
A	GDP201
A	HOH301
A	HOH302
A	HOH304
A	HOH305

---

site_id	AC3
Number of Residues	21
Details	binding site for residue GDP C 201

Chain	Residue
C	GLY13
C	VAL14
C	GLY15
C	LYS16
C	SER17
C	ALA18
C	PHE28
C	VAL29
C	ASP30
C	GLU31
C	TYR32
C	ASN116
C	LYS117
C	ASP119
C	SER145
C	ALA146
C	LYS147
C	MG202
C	HOH301
C	HOH302
C	HOH304

---

site_id	AC4
Number of Residues	6
Details	binding site for residue MG C 202

Chain	Residue
C	SER17
C	GDP201
C	HOH301
C	HOH302
C	HOH303
C	HOH304

---

site_id	AC5
Number of Residues	22
Details	binding site for residue GDP E 201

Chain	Residue
E	GLY13
E	VAL14
E	GLY15
E	LYS16
E	SER17
E	ALA18
E	PHE28
E	VAL29
E	ASP30
E	GLU31
E	TYR32
E	ASN116
E	LYS117
E	ASP119
E	LEU120
E	SER145
E	ALA146
E	LYS147
E	MG202
E	HOH301
E	HOH302
E	HOH303

---

site_id	AC6
Number of Residues	6
Details	binding site for residue MG E 202

Chain	Residue
E	SER17
E	GDP201
E	HOH301
E	HOH302
E	HOH303
E	HOH304

---

site_id	AC7
Number of Residues	22
Details	binding site for residue GDP G 201

Chain	Residue
G	LYS147
G	MG202
G	HOH302
G	HOH303
G	HOH304
G	ALA11
G	GLY13
G	VAL14
G	GLY15
G	LYS16
G	SER17
G	ALA18
G	PHE28
G	VAL29
G	ASP30
G	TYR32
G	ASN116
G	LYS117
G	ASP119
G	LEU120
G	SER145
G	ALA146

---

site_id	AC8
Number of Residues	8
Details	binding site for residue MG G 202

Chain	Residue
G	SER17
G	ASP33
G	PRO34
G	GDP201
G	HOH301
G	HOH302
G	HOH303
G	HOH304

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	16
Details	BINDING: BINDING => ECO:0000269|PubMed:22431598, ECO:0000269|PubMed:22566140

Chain	Residue	Details
A	GLY10
A	VAL29
A	ALA59
A	ASN116
C	GLY10
C	VAL29
C	ALA59
C	ASN116
E	GLY10
E	VAL29
E	ALA59
E	ASN116
G	GLY10
G	VAL29
G	ALA59
G	ASN116

---

site_id	SWS_FT_FI2
Number of Residues	4
Details	MOD_RES: N-acetylmethionine; in GTPase KRas; alternate => ECO:0000269|Ref.17

Chain	Residue	Details
A	MET1
C	MET1
E	MET1
G	MET1

---

site_id	SWS_FT_FI3
Number of Residues	4
Details	MOD_RES: N-acetylthreonine; in GTPase KRas, N-terminally processed => ECO:0000269|Ref.17

Chain	Residue	Details
A	THR2
C	THR2
E	THR2
G	THR2

---

site_id	SWS_FT_FI4
Number of Residues	4
Details	MOD_RES: N6-acetyllysine => ECO:0000269|PubMed:22711838

Chain	Residue	Details
A	LYS104
C	LYS104
E	LYS104
G	LYS104

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	CARBOHYD: (Microbial infection) O-linked (Glc) threonine; by P.sordellii toxin TcsL => ECO:0000269|PubMed:19744486

Chain	Residue	Details
E	THR35
A	THR35
C	THR35
G	THR35

---