5NBM

Crystal structure of the Arp4-N-actin(ATP-state) heterodimer bound by a nanobody

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000785	cellular_component	chromatin
A	0000812	cellular_component	Swr1 complex
A	0003682	molecular_function	chromatin binding
A	0005515	molecular_function	protein binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0006281	biological_process	DNA repair
A	0006325	biological_process	chromatin organization
A	0006338	biological_process	chromatin remodeling
A	0006351	biological_process	DNA-templated transcription
A	0006355	biological_process	regulation of DNA-templated transcription
A	0006357	biological_process	regulation of transcription by RNA polymerase II
A	0016514	cellular_component	SWI/SNF complex
A	0031011	cellular_component	Ino80 complex
A	0035267	cellular_component	NuA4 histone acetyltransferase complex
A	0042393	molecular_function	histone binding
A	0043232	cellular_component	intracellular non-membrane-bounded organelle
A	0051382	biological_process	kinetochore assembly
B	0000785	cellular_component	chromatin
B	0000812	cellular_component	Swr1 complex
B	0003682	molecular_function	chromatin binding
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0006281	biological_process	DNA repair
B	0006325	biological_process	chromatin organization
B	0006338	biological_process	chromatin remodeling
B	0006351	biological_process	DNA-templated transcription
B	0006355	biological_process	regulation of DNA-templated transcription
B	0006357	biological_process	regulation of transcription by RNA polymerase II
B	0016514	cellular_component	SWI/SNF complex
B	0031011	cellular_component	Ino80 complex
B	0035267	cellular_component	NuA4 histone acetyltransferase complex
B	0042393	molecular_function	histone binding
B	0043232	cellular_component	intracellular non-membrane-bounded organelle
B	0051382	biological_process	kinetochore assembly
C	0000011	biological_process	vacuole inheritance
C	0000142	cellular_component	cellular bud neck contractile ring
C	0000785	cellular_component	chromatin
C	0000812	cellular_component	Swr1 complex
C	0005200	molecular_function	structural constituent of cytoskeleton
C	0005515	molecular_function	protein binding
C	0005524	molecular_function	ATP binding
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005856	cellular_component	cytoskeleton
C	0005884	cellular_component	actin filament
C	0006281	biological_process	DNA repair
C	0006338	biological_process	chromatin remodeling
C	0006351	biological_process	DNA-templated transcription
C	0006355	biological_process	regulation of DNA-templated transcription
C	0006897	biological_process	endocytosis
C	0009306	biological_process	protein secretion
C	0016787	molecular_function	hydrolase activity
C	0016887	molecular_function	ATP hydrolysis activity
C	0030010	biological_process	establishment of cell polarity
C	0030476	biological_process	ascospore wall assembly
C	0030479	cellular_component	actin cortical patch
C	0031011	cellular_component	Ino80 complex
C	0032432	cellular_component	actin filament bundle
C	0035267	cellular_component	NuA4 histone acetyltransferase complex
C	0042802	molecular_function	identical protein binding
C	0043232	cellular_component	intracellular non-membrane-bounded organelle
C	1902404	biological_process	mitotic actomyosin contractile ring contraction
D	0000011	biological_process	vacuole inheritance
D	0000142	cellular_component	cellular bud neck contractile ring
D	0000785	cellular_component	chromatin
D	0000812	cellular_component	Swr1 complex
D	0005200	molecular_function	structural constituent of cytoskeleton
D	0005515	molecular_function	protein binding
D	0005524	molecular_function	ATP binding
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005856	cellular_component	cytoskeleton
D	0005884	cellular_component	actin filament
D	0006281	biological_process	DNA repair
D	0006338	biological_process	chromatin remodeling
D	0006351	biological_process	DNA-templated transcription
D	0006355	biological_process	regulation of DNA-templated transcription
D	0006897	biological_process	endocytosis
D	0009306	biological_process	protein secretion
D	0016787	molecular_function	hydrolase activity
D	0016887	molecular_function	ATP hydrolysis activity
D	0030010	biological_process	establishment of cell polarity
D	0030476	biological_process	ascospore wall assembly
D	0030479	cellular_component	actin cortical patch
D	0031011	cellular_component	Ino80 complex
D	0032432	cellular_component	actin filament bundle
D	0035267	cellular_component	NuA4 histone acetyltransferase complex
D	0042802	molecular_function	identical protein binding
D	0043232	cellular_component	intracellular non-membrane-bounded organelle
D	1902404	biological_process	mitotic actomyosin contractile ring contraction

Functional Information from PDB Data

site_id	AC1
Number of Residues	2
Details	binding site for residue CA A 501

Chain	Residue
A	ASP159
A	ATP502

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site_id	AC2
Number of Residues	14
Details	binding site for residue ATP A 502

Chain	Residue
A	GLN240
A	LYS243
A	GLY412
A	GLY413
A	THR414
A	GLN449
A	CA501
A	GLY22
A	SER23
A	TYR24
A	THR25
A	GLY161
A	HIS162
A	ASP163

---

site_id	AC3
Number of Residues	2
Details	binding site for residue CA B 501

Chain	Residue
B	ASP159
B	ATP502

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site_id	AC4
Number of Residues	15
Details	binding site for residue ATP B 502

Chain	Residue
B	GLY22
B	SER23
B	TYR24
B	THR25
B	HIS162
B	ASP163
B	THR164
B	GLN240
B	LYS243
B	GLY412
B	GLY413
B	THR414
B	ILE417
B	GLN449
B	CA501

---

site_id	AC5
Number of Residues	2
Details	binding site for residue CA C 501

Chain	Residue
C	GLN137
C	ATP502

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site_id	AC6
Number of Residues	19
Details	binding site for residue ATP C 502

Chain	Residue
C	GLY13
C	SER14
C	GLY15
C	MET16
C	LYS18
C	GLY156
C	ASP157
C	GLY158
C	VAL159
C	ARG210
C	LYS213
C	GLU214
C	GLY301
C	GLY302
C	THR303
C	MET305
C	PHE306
C	LYS336
C	CA501

---

site_id	AC7
Number of Residues	2
Details	binding site for residue CA D 501

Chain	Residue
D	GLN137
D	ATP502

---

site_id	AC8
Number of Residues	19
Details	binding site for residue ATP D 502

Chain	Residue
D	GLY13
D	SER14
D	GLY15
D	MET16
D	LYS18
D	GLY156
D	ASP157
D	GLY158
D	VAL159
D	ARG210
D	LYS213
D	GLU214
D	GLY301
D	GLY302
D	THR303
D	MET305
D	PHE306
D	LYS336
D	CA501

---

Functional Information from PROSITE/UniProt

site_id	PS00406
Number of Residues	11
Details	ACTINS_1 Actins signature 1. YVGDEAQs.KRG

Chain	Residue	Details
C	TYR53-GLY63

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site_id	PS00432
Number of Residues	9
Details	ACTINS_2 Actins signature 2. WISKqEYDE

Chain	Residue	Details
C	TRP356-GLU364

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site_id	PS01132
Number of Residues	13
Details	ACTINS_ACT_LIKE Actins and actin-related proteins signature. LLTEApMNPksNR

Chain	Residue	Details
C	LEU104-ARG116
A	LEU109-ARG121

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	SITE: Not methylated => ECO:0000269|PubMed:10496983

Chain	Residue	Details
C	HIC73
D	HIC73

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site_id	SWS_FT_FI2
Number of Residues	2
Details	MOD_RES: N-acetylmethionine => ECO:0000269|PubMed:1885608, ECO:0007744|PubMed:22814378

Chain	Residue	Details
C	MET1
D	MET1

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site_id	SWS_FT_FI3
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0007744|PubMed:22106047

Chain	Residue	Details
C	LYS191
D	LYS191

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