5NBM
Crystal structure of the Arp4-N-actin(ATP-state) heterodimer bound by a nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-06-29 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1 |
| Spacegroup name | P 65 |
| Unit cell lengths | 190.581, 190.581, 220.616 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.735 - 3.400 |
| R-factor | 0.1539 |
| Rwork | 0.152 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.659 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.25) |
| Phasing software | PHASER (2.6.1) |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.230 | 49.230 | 3.490 |
| High resolution limit [Å] | 3.400 | 15.210 | 3.400 |
| Rmerge | 0.165 | 0.036 | 1.113 |
| Rmeas | 0.180 | 0.040 | 1.206 |
| Rpim | 0.070 | 0.016 | 0.463 |
| Total number of observations | 405414 | ||
| Number of reflections | 62339 | ||
| <I/σ(I)> | 11.3 | ||
| Completeness [%] | 99.9 | 96.5 | 100 |
| Redundancy | 6.5 | 5.9 | 6.8 |
| CC(1/2) | 0.996 | 0.999 | 0.696 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 1 mM ATP, 0.2 mM CaCl2, 1.3-1.5 M sodium malonate pH 6.0, Subtilisin (1/6000; w(subtilisin)/w(protein)) |
