5MLS

Thrombin Mutant A190S in complex with (S)-1-(D-phenylalanyl)-N-(3-chlorobenzyl)pyrrolidine-2-carboxamide

Functional Information from GO Data

Chain	GOid	namespace	contents
H	0004252	molecular_function	serine-type endopeptidase activity
H	0005509	molecular_function	calcium ion binding
H	0006508	biological_process	proteolysis
H	0007596	biological_process	blood coagulation
L	0004252	molecular_function	serine-type endopeptidase activity
L	0005576	cellular_component	extracellular region
L	0006508	biological_process	proteolysis
L	0007596	biological_process	blood coagulation

Functional Information from PDB Data

site_id	AC1
Number of Residues	12
Details	binding site for residue 22U H 301

Chain	Residue
H	HIS57
H	PHE227
H	HOH454
H	HOH499
H	TYR60
H	GLU97
H	SER190
H	SER195
H	VAL213
H	SER214
H	TRP215
H	GLY216

---

site_id	AC2
Number of Residues	4
Details	binding site for residue DMS H 302

Chain	Residue
H	LEU130
H	ARG165
H	PHE181
H	HOH437

---

site_id	AC3
Number of Residues	8
Details	binding site for residue PO4 H 303

Chain	Residue
H	ASN95
H	ASP100
H	ARG101
H	ARG175
H	GOL306
H	GOL307
H	HOH422
H	HOH471

---

site_id	AC4
Number of Residues	6
Details	binding site for residue GOL H 304

Chain	Residue
H	ARG93
H	ARG165
H	ASP178
H	HIS230
H	GOL306
H	HOH429

---

site_id	AC5
Number of Residues	3
Details	binding site for residue GOL H 305

Chain	Residue
H	ARG173
H	PHE204
H	HOH421

---

site_id	AC6
Number of Residues	8
Details	binding site for residue GOL H 306

Chain	Residue
H	ARG93
H	ARG165
H	ILE176
H	THR177
H	ASP178
H	PO4303
H	GOL304
H	HOH474

---

site_id	AC7
Number of Residues	4
Details	binding site for residue GOL H 307

Chain	Residue
H	ARG93
H	ASN95
H	PO4303
H	HOH406

---

site_id	AC8
Number of Residues	6
Details	binding site for residue GOL H 308

Chain	Residue
H	ASP125
H	THR128
H	ASP170
H	HOH414
H	HOH435
H	HOH439

---

site_id	AC9
Number of Residues	6
Details	binding site for residue GOL H 309

Chain	Residue
H	LEU41
H	CYS42
H	LYS60
H	GLU192
H	GLY193
H	SER195

---

site_id	AD1
Number of Residues	6
Details	binding site for residue NA H 310

Chain	Residue
H	LYS169
H	THR172
H	PHE204
H	HOH457
H	HOH472
H	HOH478

---

site_id	AD2
Number of Residues	6
Details	binding site for residue NA H 311

Chain	Residue
H	ARG221
H	LYS224
H	HOH470
H	HOH479
H	HOH483
H	HOH538

---

Functional Information from PROSITE/UniProt

site_id	PS00134
Number of Residues	6
Details	TRYPSIN_HIS Serine proteases, trypsin family, histidine active site. LTAAHC

Chain	Residue	Details
H	LEU53-CYS58

---

site_id	PS00135
Number of Residues	12
Details	TRYPSIN_SER Serine proteases, trypsin family, serine active site. DSceGDSGGPFV

Chain	Residue	Details
H	ASP189-VAL200

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	22
Details	Region: {"description":"High affinity receptor-binding region which is also known as the TP508 peptide"}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	3
Details	Active site: {"description":"Charge relay system"}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	1
Details	Glycosylation: {"description":"N-linked (GlcNAc...) (complex) asparagine","evidences":[{"source":"PubMed","id":"16335952","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19139490","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19159218","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19838169","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"873923","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

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