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5M70

Crystal Structure of human RhoGAP mutated in its arginin finger (R85A) in complex with RhoA.GDP.AlF4- human

Functional Information from GO Data
ChainGOidnamespacecontents
A0007165biological_processsignal transduction
B0000902biological_processcell morphogenesis
B0001764biological_processneuron migration
B0001822biological_processkidney development
B0001998biological_processangiotensin-mediated vasoconstriction involved in regulation of systemic arterial blood pressure
B0002363biological_processalpha-beta T cell lineage commitment
B0003100biological_processregulation of systemic arterial blood pressure by endothelin
B0003189biological_processaortic valve formation
B0003924molecular_functionGTPase activity
B0003925molecular_functionG protein activity
B0005515molecular_functionprotein binding
B0005525molecular_functionGTP binding
B0005634cellular_componentnucleus
B0005737cellular_componentcytoplasm
B0005768cellular_componentendosome
B0005789cellular_componentendoplasmic reticulum membrane
B0005829cellular_componentcytosol
B0005856cellular_componentcytoskeleton
B0005886cellular_componentplasma membrane
B0005925cellular_componentfocal adhesion
B0005938cellular_componentcell cortex
B0006357biological_processregulation of transcription by RNA polymerase II
B0007010biological_processcytoskeleton organization
B0007155biological_processcell adhesion
B0007160biological_processcell-matrix adhesion
B0007264biological_processsmall GTPase-mediated signal transduction
B0007266biological_processRho protein signal transduction
B0007519biological_processskeletal muscle tissue development
B0009898cellular_componentcytoplasmic side of plasma membrane
B0010812biological_processnegative regulation of cell-substrate adhesion
B0010975biological_processregulation of neuron projection development
B0016477biological_processcell migration
B0016787molecular_functionhydrolase activity
B0017022molecular_functionmyosin binding
B0019901molecular_functionprotein kinase binding
B0021762biological_processsubstantia nigra development
B0021795biological_processcerebral cortex cell migration
B0021861biological_processforebrain radial glial cell differentiation
B0030027cellular_componentlamellipodium
B0030036biological_processactin cytoskeleton organization
B0030054cellular_componentcell junction
B0030154biological_processcell differentiation
B0030334biological_processregulation of cell migration
B0030425cellular_componentdendrite
B0030496cellular_componentmidbody
B0030521biological_processandrogen receptor signaling pathway
B0030667cellular_componentsecretory granule membrane
B0031122biological_processcytoplasmic microtubule organization
B0031982cellular_componentvesicle
B0032154cellular_componentcleavage furrow
B0032467biological_processpositive regulation of cytokinesis
B0032587cellular_componentruffle membrane
B0032956biological_processregulation of actin cytoskeleton organization
B0033144biological_processnegative regulation of intracellular steroid hormone receptor signaling pathway
B0033688biological_processregulation of osteoblast proliferation
B0034329biological_processcell junction assembly
B0034446biological_processsubstrate adhesion-dependent cell spreading
B0035385biological_processRoundabout signaling pathway
B0036089biological_processcleavage furrow formation
B0038027biological_processapolipoprotein A-I-mediated signaling pathway
B0042476biological_processodontogenesis
B0042995cellular_componentcell projection
B0043123biological_processpositive regulation of canonical NF-kappaB signal transduction
B0043149biological_processstress fiber assembly
B0043197cellular_componentdendritic spine
B0043296cellular_componentapical junction complex
B0043297biological_processapical junction assembly
B0043366biological_processbeta selection
B0043542biological_processendothelial cell migration
B0043931biological_processossification involved in bone maturation
B0044319biological_processwound healing, spreading of cells
B0045198biological_processestablishment of epithelial cell apical/basal polarity
B0045666biological_processpositive regulation of neuron differentiation
B0045792biological_processnegative regulation of cell size
B0046638biological_processpositive regulation of alpha-beta T cell differentiation
B0050919biological_processnegative chemotaxis
B0051301biological_processcell division
B0051496biological_processpositive regulation of stress fiber assembly
B0051893biological_processregulation of focal adhesion assembly
B0060071biological_processWnt signaling pathway, planar cell polarity pathway
B0060193biological_processpositive regulation of lipase activity
B0061430biological_processbone trabecula morphogenesis
B0070062cellular_componentextracellular exosome
B0070507biological_processregulation of microtubule cytoskeleton organization
B0071222biological_processcellular response to lipopolysaccharide
B0071345biological_processcellular response to cytokine stimulus
B0071526biological_processsemaphorin-plexin signaling pathway
B0071803biological_processpositive regulation of podosome assembly
B0071902biological_processpositive regulation of protein serine/threonine kinase activity
B0071944cellular_componentcell periphery
B0090051biological_processnegative regulation of cell migration involved in sprouting angiogenesis
B0090307biological_processmitotic spindle assembly
B0090324biological_processnegative regulation of oxidative phosphorylation
B0097049biological_processmotor neuron apoptotic process
B0097498biological_processendothelial tube lumen extension
B0098794cellular_componentpostsynapse
B0098978cellular_componentglutamatergic synapse
B0099159biological_processregulation of modification of postsynaptic structure
B0101003cellular_componentficolin-1-rich granule membrane
B1901224biological_processpositive regulation of non-canonical NF-kappaB signal transduction
B1902766biological_processskeletal muscle satellite cell migration
B1903427biological_processnegative regulation of reactive oxygen species biosynthetic process
B1903673biological_processmitotic cleavage furrow formation
B1904695biological_processpositive regulation of vascular associated smooth muscle contraction
B1904996biological_processpositive regulation of leukocyte adhesion to vascular endothelial cell
B1905274biological_processregulation of modification of postsynaptic actin cytoskeleton
B1990869biological_processcellular response to chemokine
B2000177biological_processregulation of neural precursor cell proliferation
B2000406biological_processpositive regulation of T cell migration
B2000672biological_processnegative regulation of motor neuron apoptotic process
F0007165biological_processsignal transduction
G0000902biological_processcell morphogenesis
G0001764biological_processneuron migration
G0001822biological_processkidney development
G0001998biological_processangiotensin-mediated vasoconstriction involved in regulation of systemic arterial blood pressure
G0002363biological_processalpha-beta T cell lineage commitment
G0003100biological_processregulation of systemic arterial blood pressure by endothelin
G0003189biological_processaortic valve formation
G0003924molecular_functionGTPase activity
G0003925molecular_functionG protein activity
G0005515molecular_functionprotein binding
G0005525molecular_functionGTP binding
G0005634cellular_componentnucleus
G0005737cellular_componentcytoplasm
G0005768cellular_componentendosome
G0005789cellular_componentendoplasmic reticulum membrane
G0005829cellular_componentcytosol
G0005856cellular_componentcytoskeleton
G0005886cellular_componentplasma membrane
G0005925cellular_componentfocal adhesion
G0005938cellular_componentcell cortex
G0006357biological_processregulation of transcription by RNA polymerase II
G0007010biological_processcytoskeleton organization
G0007155biological_processcell adhesion
G0007160biological_processcell-matrix adhesion
G0007264biological_processsmall GTPase-mediated signal transduction
G0007266biological_processRho protein signal transduction
G0007519biological_processskeletal muscle tissue development
G0009898cellular_componentcytoplasmic side of plasma membrane
G0010812biological_processnegative regulation of cell-substrate adhesion
G0010975biological_processregulation of neuron projection development
G0016477biological_processcell migration
G0016787molecular_functionhydrolase activity
G0017022molecular_functionmyosin binding
G0019901molecular_functionprotein kinase binding
G0021762biological_processsubstantia nigra development
G0021795biological_processcerebral cortex cell migration
G0021861biological_processforebrain radial glial cell differentiation
G0030027cellular_componentlamellipodium
G0030036biological_processactin cytoskeleton organization
G0030054cellular_componentcell junction
G0030154biological_processcell differentiation
G0030334biological_processregulation of cell migration
G0030425cellular_componentdendrite
G0030496cellular_componentmidbody
G0030521biological_processandrogen receptor signaling pathway
G0030667cellular_componentsecretory granule membrane
G0031122biological_processcytoplasmic microtubule organization
G0031982cellular_componentvesicle
G0032154cellular_componentcleavage furrow
G0032467biological_processpositive regulation of cytokinesis
G0032587cellular_componentruffle membrane
G0032956biological_processregulation of actin cytoskeleton organization
G0033144biological_processnegative regulation of intracellular steroid hormone receptor signaling pathway
G0033688biological_processregulation of osteoblast proliferation
G0034329biological_processcell junction assembly
G0034446biological_processsubstrate adhesion-dependent cell spreading
G0035385biological_processRoundabout signaling pathway
G0036089biological_processcleavage furrow formation
G0038027biological_processapolipoprotein A-I-mediated signaling pathway
G0042476biological_processodontogenesis
G0042995cellular_componentcell projection
G0043123biological_processpositive regulation of canonical NF-kappaB signal transduction
G0043149biological_processstress fiber assembly
G0043197cellular_componentdendritic spine
G0043296cellular_componentapical junction complex
G0043297biological_processapical junction assembly
G0043366biological_processbeta selection
G0043542biological_processendothelial cell migration
G0043931biological_processossification involved in bone maturation
G0044319biological_processwound healing, spreading of cells
G0045198biological_processestablishment of epithelial cell apical/basal polarity
G0045666biological_processpositive regulation of neuron differentiation
G0045792biological_processnegative regulation of cell size
G0046638biological_processpositive regulation of alpha-beta T cell differentiation
G0050919biological_processnegative chemotaxis
G0051301biological_processcell division
G0051496biological_processpositive regulation of stress fiber assembly
G0051893biological_processregulation of focal adhesion assembly
G0060071biological_processWnt signaling pathway, planar cell polarity pathway
G0060193biological_processpositive regulation of lipase activity
G0061430biological_processbone trabecula morphogenesis
G0070062cellular_componentextracellular exosome
G0070507biological_processregulation of microtubule cytoskeleton organization
G0071222biological_processcellular response to lipopolysaccharide
G0071345biological_processcellular response to cytokine stimulus
G0071526biological_processsemaphorin-plexin signaling pathway
G0071803biological_processpositive regulation of podosome assembly
G0071902biological_processpositive regulation of protein serine/threonine kinase activity
G0071944cellular_componentcell periphery
G0090051biological_processnegative regulation of cell migration involved in sprouting angiogenesis
G0090307biological_processmitotic spindle assembly
G0090324biological_processnegative regulation of oxidative phosphorylation
G0097049biological_processmotor neuron apoptotic process
G0097498biological_processendothelial tube lumen extension
G0098794cellular_componentpostsynapse
G0098978cellular_componentglutamatergic synapse
G0099159biological_processregulation of modification of postsynaptic structure
G0101003cellular_componentficolin-1-rich granule membrane
G1901224biological_processpositive regulation of non-canonical NF-kappaB signal transduction
G1902766biological_processskeletal muscle satellite cell migration
G1903427biological_processnegative regulation of reactive oxygen species biosynthetic process
G1903673biological_processmitotic cleavage furrow formation
G1904695biological_processpositive regulation of vascular associated smooth muscle contraction
G1904996biological_processpositive regulation of leukocyte adhesion to vascular endothelial cell
G1905274biological_processregulation of modification of postsynaptic actin cytoskeleton
G1990869biological_processcellular response to chemokine
G2000177biological_processregulation of neural precursor cell proliferation
G2000406biological_processpositive regulation of T cell migration
G2000672biological_processnegative regulation of motor neuron apoptotic process
Functional Information from PDB Data
site_idAC1
Number of Residues6
Detailsbinding site for residue MG B 201
ChainResidue
BTHR19
BTHR37
BGDP202
BALF203
BHOH309
BHOH317

site_idAC2
Number of Residues20
Detailsbinding site for residue GDP B 202
ChainResidue
BLYS18
BTHR19
BCYS20
BPHE30
BTYR34
BLYS118
BASP120
BLEU121
BSER160
BALA161
BLYS162
BMG201
BALF203
BHOH306
BHOH309
BHOH317
BHOH319
BALA15
BCYS16
BGLY17

site_idAC3
Number of Residues15
Detailsbinding site for residue ALF B 203
ChainResidue
BGLY14
BALA15
BLYS18
BTYR34
BPRO36
BTHR37
BTHR60
BALA61
BGLY62
BGLN63
BMG201
BGDP202
BHOH303
BHOH309
BHOH317

site_idAC4
Number of Residues6
Detailsbinding site for residue MG G 201
ChainResidue
GTHR19
GTHR37
GGDP202
GALF203
GHOH305
GHOH306

site_idAC5
Number of Residues20
Detailsbinding site for residue GDP G 202
ChainResidue
GALA15
GCYS16
GGLY17
GLYS18
GTHR19
GCYS20
GPHE30
GTYR34
GLYS118
GASP120
GLEU121
GSER160
GALA161
GLYS162
GMG201
GALF203
GHOH305
GHOH306
GHOH311
GHOH317

site_idAC6
Number of Residues14
Detailsbinding site for residue ALF G 203
ChainResidue
GGLY14
GALA15
GLYS18
GTYR34
GPRO36
GTHR37
GTHR60
GALA61
GGLY62
GGLN63
GMG201
GGDP202
GHOH303
GHOH305

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues4
DetailsBINDING: BINDING => ECO:0000269|PubMed:10748207, ECO:0000269|PubMed:12777804
ChainResidueDetails
BGLY12
BASN117
GGLY12
GASN117

site_idSWS_FT_FI2
Number of Residues6
DetailsBINDING: BINDING => ECO:0000250|UniProtKB:P62820
ChainResidueDetails
BPHE30
BASP59
BSER160
GPHE30
GASP59
GSER160

site_idSWS_FT_FI3
Number of Residues2
DetailsSITE: (Microbial infection) Cleavage; by yopT => ECO:0000269|PubMed:12062101, ECO:0000269|PubMed:12538863
ChainResidueDetails
BGLY189
GGLY189

site_idSWS_FT_FI4
Number of Residues2
DetailsMOD_RES: (Microbial infection) O-AMP-tyrosine; by Haemophilus IbpA; alternate => ECO:0000269|PubMed:19362538
ChainResidueDetails
BTYR34
GTYR34

site_idSWS_FT_FI5
Number of Residues2
DetailsMOD_RES: (Microbial infection) O-AMP-threonine; by Vibrio VopS => ECO:0000269|PubMed:19039103
ChainResidueDetails
BTHR37
GTHR37

site_idSWS_FT_FI6
Number of Residues2
DetailsMOD_RES: (Microbial infection) ADP-ribosylasparagine; by botulinum toxin => ECO:0000305|PubMed:1328215
ChainResidueDetails
BASN41
GASN41

site_idSWS_FT_FI7
Number of Residues2
DetailsMOD_RES: 5-glutamyl serotonin => ECO:0000250|UniProtKB:Q9QUI0
ChainResidueDetails
BGLN63
GGLN63

site_idSWS_FT_FI8
Number of Residues2
DetailsMOD_RES: Phosphoserine; by PKG/PRKG1 => ECO:0000269|PubMed:11162591
ChainResidueDetails
BSER188
GSER188

site_idSWS_FT_FI9
Number of Residues2
DetailsMOD_RES: Cysteine methyl ester => ECO:0000250|UniProtKB:P61585
ChainResidueDetails
BCYS190
GCYS190

site_idSWS_FT_FI10
Number of Residues6
DetailsLIPID: (Microbial infection) N6-stearoyl lysine => ECO:0000269|PubMed:30061757
ChainResidueDetails
BLYS185
BLYS186
BLYS187
GLYS185
GLYS186
GLYS187

site_idSWS_FT_FI11
Number of Residues2
DetailsLIPID: S-geranylgeranyl cysteine => ECO:0000250|UniProtKB:P61585
ChainResidueDetails
BCYS190
GCYS190

site_idSWS_FT_FI12
Number of Residues2
DetailsCARBOHYD: (Microbial infection) O-linked (GlcNAc) tyrosine; by Photorhabdus PAU_02230; alternate => ECO:0000269|PubMed:24141704
ChainResidueDetails
BTYR34
GTYR34

site_idSWS_FT_FI13
Number of Residues2
DetailsCARBOHYD: (Microbial infection) O-linked (Glc) threonine; by C.difficile toxins TcdA and TcdB; alternate => ECO:0000269|PubMed:24905543, ECO:0000269|PubMed:7775453, ECO:0000269|PubMed:7777059
ChainResidueDetails
BTHR37
GTHR37

site_idSWS_FT_FI14
Number of Residues4
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) => ECO:0000269|PubMed:23871831
ChainResidueDetails
BLYS135
GLYS135

223532

PDB entries from 2024-08-07

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