5M70
Crystal Structure of human RhoGAP mutated in its arginin finger (R85A) in complex with RhoA.GDP.AlF4- human
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.976 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.554, 66.063, 76.759 |
| Unit cell angles | 90.00, 96.07, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.200 |
| R-factor | 0.24008 |
| Rwork | 0.238 |
| R-free | 0.27652 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tx4 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.185 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.720 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.077 | 0.502 |
| Number of reflections | 34416 | |
| <I/σ(I)> | 7.9 | |
| Completeness [%] | 98.5 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 293 | 100 mM Bis/Tris pH 5.8-6 PEG3350 23-26% |
