5KZ5

Architecture of the Human Mitochondrial Iron-Sulfur Cluster Assembly Machinery: the Complex Formed by the Iron Donor, the Sulfur Donor, and the Scaffold

Functional Information from GO Data

Chain	GOid	namespace	contents
1	0030170	molecular_function	pyridoxal phosphate binding
1	0031071	molecular_function	cysteine desulfurase activity
1	0044571	biological_process	[2Fe-2S] cluster assembly
2	0030170	molecular_function	pyridoxal phosphate binding
2	0031071	molecular_function	cysteine desulfurase activity
2	0044571	biological_process	[2Fe-2S] cluster assembly
3	0030170	molecular_function	pyridoxal phosphate binding
3	0031071	molecular_function	cysteine desulfurase activity
3	0044571	biological_process	[2Fe-2S] cluster assembly
4	0030170	molecular_function	pyridoxal phosphate binding
4	0031071	molecular_function	cysteine desulfurase activity
4	0044571	biological_process	[2Fe-2S] cluster assembly
a	0005506	molecular_function	iron ion binding
a	0016226	biological_process	iron-sulfur cluster assembly
a	0051536	molecular_function	iron-sulfur cluster binding
A	0004322	molecular_function	ferroxidase activity
A	0005739	cellular_component	mitochondrion
A	0006879	biological_process	intracellular iron ion homeostasis
A	0008199	molecular_function	ferric iron binding
A	0016226	biological_process	iron-sulfur cluster assembly
A	0018283	biological_process	iron incorporation into metallo-sulfur cluster
b	0005506	molecular_function	iron ion binding
b	0016226	biological_process	iron-sulfur cluster assembly
b	0051536	molecular_function	iron-sulfur cluster binding
B	0004322	molecular_function	ferroxidase activity
B	0005739	cellular_component	mitochondrion
B	0006879	biological_process	intracellular iron ion homeostasis
B	0008199	molecular_function	ferric iron binding
B	0016226	biological_process	iron-sulfur cluster assembly
B	0018283	biological_process	iron incorporation into metallo-sulfur cluster
c	0005506	molecular_function	iron ion binding
c	0016226	biological_process	iron-sulfur cluster assembly
c	0051536	molecular_function	iron-sulfur cluster binding
C	0004322	molecular_function	ferroxidase activity
C	0005739	cellular_component	mitochondrion
C	0006879	biological_process	intracellular iron ion homeostasis
C	0008199	molecular_function	ferric iron binding
C	0016226	biological_process	iron-sulfur cluster assembly
C	0018283	biological_process	iron incorporation into metallo-sulfur cluster
d	0005506	molecular_function	iron ion binding
d	0016226	biological_process	iron-sulfur cluster assembly
d	0051536	molecular_function	iron-sulfur cluster binding
D	0004322	molecular_function	ferroxidase activity
D	0005739	cellular_component	mitochondrion
D	0006879	biological_process	intracellular iron ion homeostasis
D	0008199	molecular_function	ferric iron binding
D	0016226	biological_process	iron-sulfur cluster assembly
D	0018283	biological_process	iron incorporation into metallo-sulfur cluster
e	0005506	molecular_function	iron ion binding
e	0016226	biological_process	iron-sulfur cluster assembly
e	0051536	molecular_function	iron-sulfur cluster binding
E	0004322	molecular_function	ferroxidase activity
E	0005739	cellular_component	mitochondrion
E	0006879	biological_process	intracellular iron ion homeostasis
E	0008199	molecular_function	ferric iron binding
E	0016226	biological_process	iron-sulfur cluster assembly
E	0018283	biological_process	iron incorporation into metallo-sulfur cluster
f	0005506	molecular_function	iron ion binding
f	0016226	biological_process	iron-sulfur cluster assembly
f	0051536	molecular_function	iron-sulfur cluster binding
F	0004322	molecular_function	ferroxidase activity
F	0005739	cellular_component	mitochondrion
F	0006879	biological_process	intracellular iron ion homeostasis
F	0008199	molecular_function	ferric iron binding
F	0016226	biological_process	iron-sulfur cluster assembly
F	0018283	biological_process	iron incorporation into metallo-sulfur cluster
g	0005506	molecular_function	iron ion binding
g	0016226	biological_process	iron-sulfur cluster assembly
g	0051536	molecular_function	iron-sulfur cluster binding
G	0004322	molecular_function	ferroxidase activity
G	0005739	cellular_component	mitochondrion
G	0006879	biological_process	intracellular iron ion homeostasis
G	0008199	molecular_function	ferric iron binding
G	0016226	biological_process	iron-sulfur cluster assembly
G	0018283	biological_process	iron incorporation into metallo-sulfur cluster
h	0005506	molecular_function	iron ion binding
h	0016226	biological_process	iron-sulfur cluster assembly
h	0051536	molecular_function	iron-sulfur cluster binding
H	0004322	molecular_function	ferroxidase activity
H	0005739	cellular_component	mitochondrion
H	0006879	biological_process	intracellular iron ion homeostasis
H	0008199	molecular_function	ferric iron binding
H	0016226	biological_process	iron-sulfur cluster assembly
H	0018283	biological_process	iron incorporation into metallo-sulfur cluster
i	0005506	molecular_function	iron ion binding
i	0016226	biological_process	iron-sulfur cluster assembly
i	0051536	molecular_function	iron-sulfur cluster binding
I	0004322	molecular_function	ferroxidase activity
I	0005739	cellular_component	mitochondrion
I	0006879	biological_process	intracellular iron ion homeostasis
I	0008199	molecular_function	ferric iron binding
I	0016226	biological_process	iron-sulfur cluster assembly
I	0018283	biological_process	iron incorporation into metallo-sulfur cluster
j	0005506	molecular_function	iron ion binding
j	0016226	biological_process	iron-sulfur cluster assembly
j	0051536	molecular_function	iron-sulfur cluster binding
J	0004322	molecular_function	ferroxidase activity
J	0005739	cellular_component	mitochondrion
J	0006879	biological_process	intracellular iron ion homeostasis
J	0008199	molecular_function	ferric iron binding
J	0016226	biological_process	iron-sulfur cluster assembly
J	0018283	biological_process	iron incorporation into metallo-sulfur cluster
k	0005506	molecular_function	iron ion binding
k	0016226	biological_process	iron-sulfur cluster assembly
k	0051536	molecular_function	iron-sulfur cluster binding
K	0004322	molecular_function	ferroxidase activity
K	0005739	cellular_component	mitochondrion
K	0006879	biological_process	intracellular iron ion homeostasis
K	0008199	molecular_function	ferric iron binding
K	0016226	biological_process	iron-sulfur cluster assembly
K	0018283	biological_process	iron incorporation into metallo-sulfur cluster
l	0005506	molecular_function	iron ion binding
l	0016226	biological_process	iron-sulfur cluster assembly
l	0051536	molecular_function	iron-sulfur cluster binding
L	0004322	molecular_function	ferroxidase activity
L	0005739	cellular_component	mitochondrion
L	0006879	biological_process	intracellular iron ion homeostasis
L	0008199	molecular_function	ferric iron binding
L	0016226	biological_process	iron-sulfur cluster assembly
L	0018283	biological_process	iron incorporation into metallo-sulfur cluster
M	0030170	molecular_function	pyridoxal phosphate binding
M	0031071	molecular_function	cysteine desulfurase activity
M	0044571	biological_process	[2Fe-2S] cluster assembly
N	0030170	molecular_function	pyridoxal phosphate binding
N	0031071	molecular_function	cysteine desulfurase activity
N	0044571	biological_process	[2Fe-2S] cluster assembly
O	0030170	molecular_function	pyridoxal phosphate binding
O	0031071	molecular_function	cysteine desulfurase activity
O	0044571	biological_process	[2Fe-2S] cluster assembly
P	0030170	molecular_function	pyridoxal phosphate binding
P	0031071	molecular_function	cysteine desulfurase activity
P	0044571	biological_process	[2Fe-2S] cluster assembly
Q	0030170	molecular_function	pyridoxal phosphate binding
Q	0031071	molecular_function	cysteine desulfurase activity
Q	0044571	biological_process	[2Fe-2S] cluster assembly
R	0030170	molecular_function	pyridoxal phosphate binding
R	0031071	molecular_function	cysteine desulfurase activity
R	0044571	biological_process	[2Fe-2S] cluster assembly
S	0030170	molecular_function	pyridoxal phosphate binding
S	0031071	molecular_function	cysteine desulfurase activity
S	0044571	biological_process	[2Fe-2S] cluster assembly
T	0030170	molecular_function	pyridoxal phosphate binding
T	0031071	molecular_function	cysteine desulfurase activity
T	0044571	biological_process	[2Fe-2S] cluster assembly

Functional Information from PROSITE/UniProt

site_id	PS00595
Number of Residues	20
Details	AA_TRANSFER_CLASS_5 Aminotransferases class-V pyridoxal-phosphate attachment site. IDLMsiSGHKiygpk.GvGaI

Chain	Residue	Details
1	ILE249-ILE268

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site_id	PS01344
Number of Residues	15
Details	FRATAXIN_1 Frataxin family signature. VINKQtPnkQIWlSS

Chain	Residue	Details
H	VAL144-SER158

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	12
Details	ACT_SITE: Cysteine persulfide intermediate => ECO:0000250|UniProtKB:Q9D7P6

Chain	Residue	Details
a	GLY94
j	GLY94
k	GLY94
l	GLY94
b	GLY94
c	GLY94
d	GLY94
e	GLY94
f	GLY94
g	GLY94
h	GLY94
i	GLY94

---

site_id	SWS_FT_FI2
Number of Residues	12
Details	ACT_SITE: Cysteine persulfide intermediate => ECO:0000250|UniProtKB:O29689

Chain	Residue	Details
a	GLU163
j	GLU163
k	GLU163
l	GLU163
b	GLU163
c	GLU163
d	GLU163
e	GLU163
f	GLU163
g	GLU163
h	GLU163
i	GLU163

---

site_id	SWS_FT_FI3
Number of Residues	36
Details	BINDING: BINDING => ECO:0000269|PubMed:31101807, ECO:0007744|PDB:6NZU

Chain	Residue	Details
a	GLY96
d	GLY96
d	ILE120
d	GLU163
e	GLY96
e	ILE120
e	GLU163
f	GLY96
f	ILE120
f	GLU163
g	GLY96
a	ILE120
g	ILE120
g	GLU163
h	GLY96
h	ILE120
h	GLU163
i	GLY96
i	ILE120
i	GLU163
j	GLY96
j	ILE120
a	GLU163
j	GLU163
k	GLY96
k	ILE120
k	GLU163
l	GLY96
l	ILE120
l	GLU163
R	THR128
R	GLN235
R	SER255
b	GLY96
R	HIS257
S	THR128
S	GLN235
S	SER255
S	HIS257
T	THR128
T	GLN235
T	SER255
T	HIS257
b	ILE120
b	GLU163
c	GLY96
c	ILE120
c	GLU163

---

site_id	SWS_FT_FI4
Number of Residues	12
Details	SITE: Mediates ISCU dimerization and de novo [2Fe-2S] cluster assembly => ECO:0000269|PubMed:34824239

Chain	Residue	Details
a	GLY60
j	GLY60
k	GLY60
l	GLY60
b	GLY60
c	GLY60
d	GLY60
e	GLY60
f	GLY60
g	GLY60
h	GLY60
i	GLY60

---

site_id	SWS_FT_FI5
Number of Residues	12
Details	MOD_RES: Cysteine persulfide => ECO:0000250|UniProtKB:Q9D7P6

Chain	Residue	Details
a	GLY94
j	GLY94
k	GLY94
l	GLY94
b	GLY94
c	GLY94
d	GLY94
e	GLY94
f	GLY94
g	GLY94
h	GLY94
i	GLY94

---

site_id	SWS_FT_FI6
Number of Residues	12
Details	MOD_RES: Cysteine persulfide => ECO:0000305|PubMed:24971490

Chain	Residue	Details
a	GLU163
j	GLU163
k	GLU163
l	GLU163
b	GLU163
c	GLU163
d	GLU163
e	GLU163
f	GLU163
g	GLU163
h	GLU163
i	GLU163

---

site_id	SWS_FT_FI7
Number of Residues	12
Details	MOD_RES: Cysteine persulfide => ECO:0000305|PubMed:18650437, ECO:0000305|PubMed:23593335

Chain	Residue	Details
1	CYS381
R	CYS381
S	CYS381
T	CYS381
2	CYS381
3	CYS381
4	CYS381
M	CYS381
N	CYS381
O	CYS381
P	CYS381
Q	CYS381

---