5KZ5
Architecture of the Human Mitochondrial Iron-Sulfur Cluster Assembly Machinery: the Complex Formed by the Iron Donor, the Sulfur Donor, and the Scaffold
EM specimen
| Staining | Yes |
| Staining material | 1% uranyl acetate |
EM Data Collection
| Microscope | FEI TECNAI F30 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2 |
| Detector | GATAN ULTRASCAN 4000 (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 14.3 |
| Number of Particle | 4124 |
| Software | DigitalMicrograph,Coot,EMAN2,UCSF Chimera,Situs,NAMD |
| Symmetry | O |
| CTF correction | PHASE FLIPPING ONLY |
