5KU6
Crystal structure for the complex of human carbonic anhydrase IV and methazolamide
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004089 | molecular_function | carbonate dehydratase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005791 | cellular_component | rough endoplasmic reticulum |
| A | 0005793 | cellular_component | endoplasmic reticulum-Golgi intermediate compartment |
| A | 0005794 | cellular_component | Golgi apparatus |
| A | 0005802 | cellular_component | trans-Golgi network |
| A | 0005886 | cellular_component | plasma membrane |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0008270 | molecular_function | zinc ion binding |
| A | 0009897 | cellular_component | external side of plasma membrane |
| A | 0009986 | cellular_component | cell surface |
| A | 0015701 | biological_process | bicarbonate transport |
| A | 0016020 | cellular_component | membrane |
| A | 0016323 | cellular_component | basolateral plasma membrane |
| A | 0016324 | cellular_component | apical plasma membrane |
| A | 0016829 | molecular_function | lyase activity |
| A | 0030658 | cellular_component | transport vesicle membrane |
| A | 0030667 | cellular_component | secretory granule membrane |
| A | 0031526 | cellular_component | brush border membrane |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0048471 | cellular_component | perinuclear region of cytoplasm |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 0098552 | cellular_component | side of membrane |
| B | 0004089 | molecular_function | carbonate dehydratase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005791 | cellular_component | rough endoplasmic reticulum |
| B | 0005793 | cellular_component | endoplasmic reticulum-Golgi intermediate compartment |
| B | 0005794 | cellular_component | Golgi apparatus |
| B | 0005802 | cellular_component | trans-Golgi network |
| B | 0005886 | cellular_component | plasma membrane |
| B | 0006730 | biological_process | one-carbon metabolic process |
| B | 0008270 | molecular_function | zinc ion binding |
| B | 0009897 | cellular_component | external side of plasma membrane |
| B | 0009986 | cellular_component | cell surface |
| B | 0015701 | biological_process | bicarbonate transport |
| B | 0016020 | cellular_component | membrane |
| B | 0016323 | cellular_component | basolateral plasma membrane |
| B | 0016324 | cellular_component | apical plasma membrane |
| B | 0016829 | molecular_function | lyase activity |
| B | 0030658 | cellular_component | transport vesicle membrane |
| B | 0030667 | cellular_component | secretory granule membrane |
| B | 0031526 | cellular_component | brush border membrane |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0048471 | cellular_component | perinuclear region of cytoplasm |
| B | 0070062 | cellular_component | extracellular exosome |
| B | 0098552 | cellular_component | side of membrane |
| C | 0004089 | molecular_function | carbonate dehydratase activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005791 | cellular_component | rough endoplasmic reticulum |
| C | 0005793 | cellular_component | endoplasmic reticulum-Golgi intermediate compartment |
| C | 0005794 | cellular_component | Golgi apparatus |
| C | 0005802 | cellular_component | trans-Golgi network |
| C | 0005886 | cellular_component | plasma membrane |
| C | 0006730 | biological_process | one-carbon metabolic process |
| C | 0008270 | molecular_function | zinc ion binding |
| C | 0009897 | cellular_component | external side of plasma membrane |
| C | 0009986 | cellular_component | cell surface |
| C | 0015701 | biological_process | bicarbonate transport |
| C | 0016020 | cellular_component | membrane |
| C | 0016323 | cellular_component | basolateral plasma membrane |
| C | 0016324 | cellular_component | apical plasma membrane |
| C | 0016829 | molecular_function | lyase activity |
| C | 0030658 | cellular_component | transport vesicle membrane |
| C | 0030667 | cellular_component | secretory granule membrane |
| C | 0031526 | cellular_component | brush border membrane |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0048471 | cellular_component | perinuclear region of cytoplasm |
| C | 0070062 | cellular_component | extracellular exosome |
| C | 0098552 | cellular_component | side of membrane |
| D | 0004089 | molecular_function | carbonate dehydratase activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005791 | cellular_component | rough endoplasmic reticulum |
| D | 0005793 | cellular_component | endoplasmic reticulum-Golgi intermediate compartment |
| D | 0005794 | cellular_component | Golgi apparatus |
| D | 0005802 | cellular_component | trans-Golgi network |
| D | 0005886 | cellular_component | plasma membrane |
| D | 0006730 | biological_process | one-carbon metabolic process |
| D | 0008270 | molecular_function | zinc ion binding |
| D | 0009897 | cellular_component | external side of plasma membrane |
| D | 0009986 | cellular_component | cell surface |
| D | 0015701 | biological_process | bicarbonate transport |
| D | 0016020 | cellular_component | membrane |
| D | 0016323 | cellular_component | basolateral plasma membrane |
| D | 0016324 | cellular_component | apical plasma membrane |
| D | 0016829 | molecular_function | lyase activity |
| D | 0030658 | cellular_component | transport vesicle membrane |
| D | 0030667 | cellular_component | secretory granule membrane |
| D | 0031526 | cellular_component | brush border membrane |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0048471 | cellular_component | perinuclear region of cytoplasm |
| D | 0070062 | cellular_component | extracellular exosome |
| D | 0098552 | cellular_component | side of membrane |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 10 |
| Details | binding site for residue MZM A 301 |
| Chain | Residue |
| A | GLN92 |
| A | HOH473 |
| A | HIS94 |
| A | HIS96 |
| A | HIS119 |
| A | LEU198 |
| A | THR199 |
| A | THR200 |
| A | TRP209 |
| A | ZN302 |
| site_id | AC2 |
| Number of Residues | 4 |
| Details | binding site for residue ZN A 302 |
| Chain | Residue |
| A | HIS94 |
| A | HIS96 |
| A | HIS119 |
| A | MZM301 |
| site_id | AC3 |
| Number of Residues | 7 |
| Details | binding site for residue SO4 A 303 |
| Chain | Residue |
| A | ARG27 |
| A | LEU195 |
| A | ARG254 |
| A | THR255 |
| A | HOH455 |
| A | HOH568 |
| B | HOH539 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | binding site for residue SO4 A 304 |
| Chain | Residue |
| A | GLY50 |
| A | TYR51 |
| A | ASP52 |
| A | LYS53 |
| A | HOH491 |
| site_id | AC5 |
| Number of Residues | 6 |
| Details | binding site for residue GOL A 305 |
| Chain | Residue |
| A | SER50 |
| A | SER78 |
| A | ILE79 |
| A | SER80 |
| A | PRO87 |
| C | GLN60 |
| site_id | AC6 |
| Number of Residues | 5 |
| Details | binding site for residue GOL B 301 |
| Chain | Residue |
| B | ASN62 |
| B | GLN92 |
| B | HIS94 |
| B | MZM302 |
| B | HOH407 |
| site_id | AC7 |
| Number of Residues | 11 |
| Details | binding site for residue MZM B 302 |
| Chain | Residue |
| B | GLN92 |
| B | HIS94 |
| B | HIS96 |
| B | HIS119 |
| B | VAL121 |
| B | LEU198 |
| B | THR199 |
| B | THR200 |
| B | GOL301 |
| B | ZN303 |
| B | HOH473 |
| site_id | AC8 |
| Number of Residues | 4 |
| Details | binding site for residue ZN B 303 |
| Chain | Residue |
| B | HIS94 |
| B | HIS96 |
| B | HIS119 |
| B | MZM302 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | binding site for residue SO4 B 304 |
| Chain | Residue |
| B | ARG27 |
| B | LEU195 |
| B | ARG254 |
| B | THR255 |
| B | HOH447 |
| B | HOH468 |
| site_id | AD1 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 B 305 |
| Chain | Residue |
| B | GLY50 |
| B | TYR51 |
| B | ASP52 |
| B | LYS53 |
| site_id | AD2 |
| Number of Residues | 4 |
| Details | binding site for residue ACT B 306 |
| Chain | Residue |
| B | LYS15 |
| D | GLU155 |
| D | GLN158 |
| D | GLU162 |
| site_id | AD3 |
| Number of Residues | 8 |
| Details | binding site for residue GOL B 307 |
| Chain | Residue |
| B | PHE48 |
| B | SER78 |
| B | ILE79 |
| B | SER80 |
| B | PRO87 |
| B | HOH505 |
| D | GLN60 |
| D | MET67 |
| site_id | AD4 |
| Number of Residues | 12 |
| Details | binding site for residue MZM C 301 |
| Chain | Residue |
| C | GLN92 |
| C | HIS94 |
| C | HIS96 |
| C | HIS119 |
| C | LEU198 |
| C | THR199 |
| C | THR200 |
| C | ZN302 |
| C | HOH418 |
| C | HOH550 |
| C | HOH585 |
| C | HOH612 |
| site_id | AD5 |
| Number of Residues | 4 |
| Details | binding site for residue ZN C 302 |
| Chain | Residue |
| C | HIS94 |
| C | HIS96 |
| C | HIS119 |
| C | MZM301 |
| site_id | AD6 |
| Number of Residues | 6 |
| Details | binding site for residue SO4 C 303 |
| Chain | Residue |
| C | ARG27 |
| C | ARG254 |
| C | THR255 |
| C | HOH432 |
| C | HOH442 |
| C | HOH551 |
| site_id | AD7 |
| Number of Residues | 7 |
| Details | binding site for residue SO4 C 304 |
| Chain | Residue |
| C | LYS53 |
| C | HOH496 |
| C | HOH526 |
| C | HOH568 |
| C | GLY50 |
| C | TYR51 |
| C | ASP52 |
| site_id | AD8 |
| Number of Residues | 4 |
| Details | binding site for residue ACT C 305 |
| Chain | Residue |
| A | ARG46 |
| A | ARG189 |
| C | ASN72 |
| C | GLN89 |
| site_id | AD9 |
| Number of Residues | 8 |
| Details | binding site for residue GOL C 306 |
| Chain | Residue |
| C | LYS103 |
| C | ASP243 |
| C | VAL245 |
| C | PRO247 |
| C | HOH438 |
| C | HOH483 |
| C | HOH515 |
| C | HOH524 |
| site_id | AE1 |
| Number of Residues | 3 |
| Details | binding site for residue GOL C 307 |
| Chain | Residue |
| C | SER50 |
| C | ASN72 |
| C | SER78 |
| site_id | AE2 |
| Number of Residues | 12 |
| Details | binding site for residue MZM D 301 |
| Chain | Residue |
| D | GLN92 |
| D | HIS94 |
| D | HIS96 |
| D | HIS119 |
| D | VAL121 |
| D | LEU198 |
| D | THR199 |
| D | THR200 |
| D | TRP209 |
| D | ZN302 |
| D | HOH401 |
| D | HOH595 |
| site_id | AE3 |
| Number of Residues | 4 |
| Details | binding site for residue ZN D 302 |
| Chain | Residue |
| D | HIS94 |
| D | HIS96 |
| D | HIS119 |
| D | MZM301 |
| site_id | AE4 |
| Number of Residues | 6 |
| Details | binding site for residue SO4 D 303 |
| Chain | Residue |
| D | ARG27 |
| D | LEU195 |
| D | ARG254 |
| D | THR255 |
| D | HOH424 |
| D | HOH448 |
| site_id | AE5 |
| Number of Residues | 6 |
| Details | binding site for residue SO4 D 304 |
| Chain | Residue |
| A | GLU2 |
| D | GLY50 |
| D | TYR51 |
| D | ASP52 |
| D | LYS53 |
| D | HOH472 |
| site_id | AE6 |
| Number of Residues | 8 |
| Details | binding site for residue ACT D 305 |
| Chain | Residue |
| D | ASP99 |
| D | LEU100 |
| D | PRO101 |
| D | ALA115 |
| D | ALA150 |
| D | ILE223 |
| D | LEU224 |
| D | HOH452 |
Functional Information from PROSITE/UniProt
| site_id | PS00162 |
| Number of Residues | 17 |
| Details | ALPHA_CA_1 Alpha-carbonic anhydrases signature. SEHsLdgehFamEMHIV |
| Chain | Residue | Details |
| A | SER105-VAL121 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton donor/acceptor => ECO:0000250|UniProtKB:P00918 |
| Chain | Residue | Details |
| A | HIS64 | |
| B | HIS64 | |
| C | HIS64 | |
| D | HIS64 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | BINDING: BINDING => ECO:0000269|PubMed:8942978 |
| Chain | Residue | Details |
| A | HIS94 | |
| D | HIS94 | |
| D | HIS96 | |
| D | HIS119 | |
| A | HIS96 | |
| A | HIS119 | |
| B | HIS94 | |
| B | HIS96 | |
| B | HIS119 | |
| C | HIS94 | |
| C | HIS96 | |
| C | HIS119 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 4 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P00918 |
| Chain | Residue | Details |
| A | THR199 | |
| B | THR199 | |
| C | THR199 | |
| D | THR199 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | LIPID: GPI-anchor amidated serine => ECO:0000269|PubMed:7625839 |
| Chain | Residue | Details |
| A | SER259 | |
| B | SER259 | |
| C | SER259 | |
| D | SER259 |
