5KA2

Protein Tyrosine Phosphatase 1B YAYA (Y152A, Y153A) mutant, open state

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004725	molecular_function	protein tyrosine phosphatase activity
A	0006470	biological_process	protein dephosphorylation
A	0016311	biological_process	dephosphorylation

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue GOL A 301

Chain	Residue
A	PRO89
A	CYS92
A	GLN123
A	MET133
A	HOH420
A	HOH438

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site_id	AC2
Number of Residues	8
Details	binding site for residue GOL A 302

Chain	Residue
A	GLU252
A	CL306
A	HOH402
A	HOH413
A	HOH523
A	GLU76
A	LEU234
A	LYS248

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site_id	AC3
Number of Residues	4
Details	binding site for residue TRS A 303

Chain	Residue
A	HIS54
A	LYS128
A	GLU129
A	GLU130

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site_id	AC4
Number of Residues	2
Details	binding site for residue CL A 304

Chain	Residue
A	ARG24
A	ARG254

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site_id	AC5
Number of Residues	4
Details	binding site for residue CL A 305

Chain	Residue
A	MET114
A	LYS116
A	GLY117
A	SER205

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site_id	AC6
Number of Residues	4
Details	binding site for residue CL A 306

Chain	Residue
A	ARG238
A	SER243
A	ASP245
A	GOL302

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site_id	AC7
Number of Residues	4
Details	binding site for residue CL A 307

Chain	Residue
A	SER216
A	ALA217
A	ARG221
A	HOH484

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site_id	AC8
Number of Residues	3
Details	binding site for residue CL A 308

Chain	Residue
A	ARG45
A	ALA122
A	HOH545

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site_id	AC9
Number of Residues	4
Details	binding site for residue CL A 309

Chain	Residue
A	ARG112
A	VAL113
A	HIS175
A	HOH468

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Functional Information from PROSITE/UniProt

site_id	PS00383
Number of Residues	11
Details	TYR_PHOSPHATASE_1 Tyrosine specific protein phosphatases active site. VHCsaGigRSG

Chain	Residue	Details
A	VAL213-GLY223

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: Phosphocysteine intermediate

Chain	Residue	Details
A	CYS215

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site_id	SWS_FT_FI2
Number of Residues	1
Details	BINDING:

Chain	Residue	Details
A	ASP181

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site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000250

Chain	Residue	Details
A	CYS215
A	GLN262

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site_id	SWS_FT_FI4
Number of Residues	1
Details	MOD_RES: N-acetylmethionine => ECO:0000269|PubMed:2546149

Chain	Residue	Details
A	MET1

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site_id	SWS_FT_FI5
Number of Residues	1
Details	MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:15592455

Chain	Residue	Details
A	TYR20

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site_id	SWS_FT_FI6
Number of Residues	1
Details	MOD_RES: Phosphoserine; by PKB/AKT1, CLK1 and CLK2 => ECO:0000269|PubMed:10480872, ECO:0000269|PubMed:11579209, ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER50

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site_id	SWS_FT_FI7
Number of Residues	1
Details	MOD_RES: Phosphotyrosine; by EGFR => ECO:0000269|PubMed:9355745

Chain	Residue	Details
A	TYR66

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site_id	SWS_FT_FI8
Number of Residues	1
Details	MOD_RES: S-nitrosocysteine; in reversibly inhibited form => ECO:0000269|PubMed:22169477

Chain	Residue	Details
A	CYS215

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site_id	SWS_FT_FI9
Number of Residues	2
Details	MOD_RES: Phosphoserine; by CLK1 and CLK2 => ECO:0000269|PubMed:10480872

Chain	Residue	Details
A	SER242
A	SER243

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site_id	SWS_FT_FI10
Number of Residues	2
Details	CROSSLNK: N,N-(cysteine-1,S-diyl)serine (Cys-Ser); in inhibited form => ECO:0000269|PubMed:12802338, ECO:0000269|PubMed:12802339

Chain	Residue	Details
A	CYS215
A	SER216

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Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 469

Chain	Residue	Details
A	ASP181	proton shuttle (general acid/base)
A	CYS215	covalent catalysis
A	ARG221	activator, electrostatic stabiliser
A	SER222	activator, electrostatic stabiliser
A	GLN262	steric role

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