5K9C
Crystal structure of human dihydroorotate dehydrogenase with ML390
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004151 | molecular_function | dihydroorotase activity |
| A | 0004152 | molecular_function | dihydroorotate dehydrogenase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005654 | cellular_component | nucleoplasm |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005743 | cellular_component | mitochondrial inner membrane |
| A | 0005829 | cellular_component | cytosol |
| A | 0006207 | biological_process | 'de novo' pyrimidine nucleobase biosynthetic process |
| A | 0006221 | biological_process | pyrimidine nucleotide biosynthetic process |
| A | 0006225 | biological_process | UDP biosynthetic process |
| A | 0009220 | biological_process | pyrimidine ribonucleotide biosynthetic process |
| A | 0016020 | cellular_component | membrane |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0016627 | molecular_function | oxidoreductase activity, acting on the CH-CH group of donors |
| A | 0044205 | biological_process | 'de novo' UMP biosynthetic process |
| A | 0106430 | molecular_function | dihydroorotate dehydrogenase (quinone) activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 25 |
| Details | binding site for residue FNR A 400 |
| Chain | Residue |
| A | ALA95 |
| A | LYS255 |
| A | THR283 |
| A | ASN284 |
| A | THR285 |
| A | SER305 |
| A | GLY306 |
| A | LEU309 |
| A | VAL333 |
| A | GLY334 |
| A | GLY335 |
| A | ALA96 |
| A | LEU355 |
| A | TYR356 |
| A | THR357 |
| A | ORO401 |
| A | HOH534 |
| A | HOH576 |
| A | GLY97 |
| A | LYS100 |
| A | GLY119 |
| A | SER120 |
| A | ASN145 |
| A | ASN181 |
| A | ASN212 |
| site_id | AC2 |
| Number of Residues | 11 |
| Details | binding site for residue ORO A 401 |
| Chain | Residue |
| A | LYS100 |
| A | ASN145 |
| A | TYR147 |
| A | GLY148 |
| A | PHE149 |
| A | ASN212 |
| A | SER215 |
| A | ASN217 |
| A | ASN284 |
| A | THR285 |
| A | FNR400 |
| site_id | AC3 |
| Number of Residues | 16 |
| Details | binding site for residue MLJ A 402 |
| Chain | Residue |
| A | MET43 |
| A | LEU46 |
| A | GLN47 |
| A | LEU50 |
| A | PRO52 |
| A | ALA55 |
| A | HIS56 |
| A | LEU58 |
| A | ALA59 |
| A | PHE62 |
| A | PHE98 |
| A | VAL134 |
| A | ARG136 |
| A | TYR356 |
| A | THR360 |
| A | PHE361 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | binding site for residue SO4 A 403 |
| Chain | Residue |
| A | ARG245 |
| A | VAL247 |
| A | HIS248 |
| A | HOH550 |
| site_id | AC5 |
| Number of Residues | 2 |
| Details | binding site for residue SO4 A 404 |
| Chain | Residue |
| A | ARG249 |
| A | HOH546 |
| site_id | AC6 |
| Number of Residues | 9 |
| Details | binding site for residue ACT A 405 |
| Chain | Residue |
| A | GLN168 |
| A | ALA169 |
| A | THR172 |
| A | LEU205 |
| A | ALA206 |
| A | ASP207 |
| A | HOH511 |
| A | HOH569 |
| A | HOH581 |
| site_id | AC7 |
| Number of Residues | 8 |
| Details | binding site for residue ACT A 406 |
| Chain | Residue |
| A | LYS307 |
| A | PRO308 |
| A | ASP311 |
| A | THR314 |
| A | GLN315 |
| A | ARG318 |
| A | HOH552 |
| A | HOH561 |
| site_id | AC8 |
| Number of Residues | 5 |
| Details | binding site for residue ACT A 407 |
| Chain | Residue |
| A | ARG160 |
| A | HOH577 |
| A | HOH635 |
| A | HOH637 |
| A | HOH689 |
| site_id | AC9 |
| Number of Residues | 5 |
| Details | binding site for residue CL A 408 |
| Chain | Residue |
| A | GLN165 |
| A | HIS248 |
| A | ARG298 |
| A | SER299 |
| A | HOH659 |
| site_id | AD1 |
| Number of Residues | 3 |
| Details | binding site for residue CL A 409 |
| Chain | Residue |
| A | ARG347 |
| A | GLN381 |
| A | HOH523 |
| site_id | AD2 |
| Number of Residues | 4 |
| Details | binding site for residue GOL A 410 |
| Chain | Residue |
| A | LYS167 |
| A | LYS227 |
| A | ARG231 |
| A | HOH520 |
Functional Information from PROSITE/UniProt
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Nucleophile"} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 12 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"10673429","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16480261","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 11 |
| Details | Binding site: {} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 7 |
| Details | M-CSA 109 |
| Chain | Residue | Details |
| A | ASN145 | electrostatic stabiliser |
| A | PHE149 | activator, electrostatic stabiliser, enhance reactivity, polar/non-polar interaction |
| A | SER215 | electrostatic stabiliser, hydrogen bond acceptor, hydrogen bond donor, polar/non-polar interaction, proton acceptor, proton donor, proton relay |
| A | ASN217 | electrostatic stabiliser |
| A | THR218 | activator, electrostatic stabiliser, enhance reactivity, hydrogen bond acceptor |
| A | LYS255 | electrostatic stabiliser, hydrogen bond donor |
| A | ASN284 | electrostatic stabiliser |
