5JKK

Crystal structure of the negatively supercharged variant Ftn(neg) of human heavy chain ferritin

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004322	molecular_function	ferroxidase activity
A	0005506	molecular_function	iron ion binding
A	0005515	molecular_function	protein binding
A	0005576	cellular_component	extracellular region
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005764	cellular_component	lysosome
A	0005776	cellular_component	autophagosome
A	0005829	cellular_component	cytosol
A	0006826	biological_process	iron ion transport
A	0006879	biological_process	intracellular iron ion homeostasis
A	0006955	biological_process	immune response
A	0008198	molecular_function	ferrous iron binding
A	0008199	molecular_function	ferric iron binding
A	0008285	biological_process	negative regulation of cell population proliferation
A	0016491	molecular_function	oxidoreductase activity
A	0031410	cellular_component	cytoplasmic vesicle
A	0042802	molecular_function	identical protein binding
A	0044754	cellular_component	autolysosome
A	0046872	molecular_function	metal ion binding
A	0048147	biological_process	negative regulation of fibroblast proliferation
A	0070062	cellular_component	extracellular exosome
A	0070288	cellular_component	ferritin complex
A	0110076	biological_process	negative regulation of ferroptosis
A	0140315	molecular_function	iron ion sequestering activity
A	1904724	cellular_component	tertiary granule lumen
A	1904813	cellular_component	ficolin-1-rich granule lumen
B	0004322	molecular_function	ferroxidase activity
B	0005506	molecular_function	iron ion binding
B	0005515	molecular_function	protein binding
B	0005576	cellular_component	extracellular region
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005764	cellular_component	lysosome
B	0005776	cellular_component	autophagosome
B	0005829	cellular_component	cytosol
B	0006826	biological_process	iron ion transport
B	0006879	biological_process	intracellular iron ion homeostasis
B	0006955	biological_process	immune response
B	0008198	molecular_function	ferrous iron binding
B	0008199	molecular_function	ferric iron binding
B	0008285	biological_process	negative regulation of cell population proliferation
B	0016491	molecular_function	oxidoreductase activity
B	0031410	cellular_component	cytoplasmic vesicle
B	0042802	molecular_function	identical protein binding
B	0044754	cellular_component	autolysosome
B	0046872	molecular_function	metal ion binding
B	0048147	biological_process	negative regulation of fibroblast proliferation
B	0070062	cellular_component	extracellular exosome
B	0070288	cellular_component	ferritin complex
B	0110076	biological_process	negative regulation of ferroptosis
B	0140315	molecular_function	iron ion sequestering activity
B	1904724	cellular_component	tertiary granule lumen
B	1904813	cellular_component	ficolin-1-rich granule lumen
C	0004322	molecular_function	ferroxidase activity
C	0005506	molecular_function	iron ion binding
C	0005515	molecular_function	protein binding
C	0005576	cellular_component	extracellular region
C	0005634	cellular_component	nucleus
C	0005737	cellular_component	cytoplasm
C	0005764	cellular_component	lysosome
C	0005776	cellular_component	autophagosome
C	0005829	cellular_component	cytosol
C	0006826	biological_process	iron ion transport
C	0006879	biological_process	intracellular iron ion homeostasis
C	0006955	biological_process	immune response
C	0008198	molecular_function	ferrous iron binding
C	0008199	molecular_function	ferric iron binding
C	0008285	biological_process	negative regulation of cell population proliferation
C	0016491	molecular_function	oxidoreductase activity
C	0031410	cellular_component	cytoplasmic vesicle
C	0042802	molecular_function	identical protein binding
C	0044754	cellular_component	autolysosome
C	0046872	molecular_function	metal ion binding
C	0048147	biological_process	negative regulation of fibroblast proliferation
C	0070062	cellular_component	extracellular exosome
C	0070288	cellular_component	ferritin complex
C	0110076	biological_process	negative regulation of ferroptosis
C	0140315	molecular_function	iron ion sequestering activity
C	1904724	cellular_component	tertiary granule lumen
C	1904813	cellular_component	ficolin-1-rich granule lumen
D	0004322	molecular_function	ferroxidase activity
D	0005506	molecular_function	iron ion binding
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005634	cellular_component	nucleus
D	0005737	cellular_component	cytoplasm
D	0005764	cellular_component	lysosome
D	0005776	cellular_component	autophagosome
D	0005829	cellular_component	cytosol
D	0006826	biological_process	iron ion transport
D	0006879	biological_process	intracellular iron ion homeostasis
D	0006955	biological_process	immune response
D	0008198	molecular_function	ferrous iron binding
D	0008199	molecular_function	ferric iron binding
D	0008285	biological_process	negative regulation of cell population proliferation
D	0016491	molecular_function	oxidoreductase activity
D	0031410	cellular_component	cytoplasmic vesicle
D	0042802	molecular_function	identical protein binding
D	0044754	cellular_component	autolysosome
D	0046872	molecular_function	metal ion binding
D	0048147	biological_process	negative regulation of fibroblast proliferation
D	0070062	cellular_component	extracellular exosome
D	0070288	cellular_component	ferritin complex
D	0110076	biological_process	negative regulation of ferroptosis
D	0140315	molecular_function	iron ion sequestering activity
D	1904724	cellular_component	tertiary granule lumen
D	1904813	cellular_component	ficolin-1-rich granule lumen
E	0004322	molecular_function	ferroxidase activity
E	0005506	molecular_function	iron ion binding
E	0005515	molecular_function	protein binding
E	0005576	cellular_component	extracellular region
E	0005634	cellular_component	nucleus
E	0005737	cellular_component	cytoplasm
E	0005764	cellular_component	lysosome
E	0005776	cellular_component	autophagosome
E	0005829	cellular_component	cytosol
E	0006826	biological_process	iron ion transport
E	0006879	biological_process	intracellular iron ion homeostasis
E	0006955	biological_process	immune response
E	0008198	molecular_function	ferrous iron binding
E	0008199	molecular_function	ferric iron binding
E	0008285	biological_process	negative regulation of cell population proliferation
E	0016491	molecular_function	oxidoreductase activity
E	0031410	cellular_component	cytoplasmic vesicle
E	0042802	molecular_function	identical protein binding
E	0044754	cellular_component	autolysosome
E	0046872	molecular_function	metal ion binding
E	0048147	biological_process	negative regulation of fibroblast proliferation
E	0070062	cellular_component	extracellular exosome
E	0070288	cellular_component	ferritin complex
E	0110076	biological_process	negative regulation of ferroptosis
E	0140315	molecular_function	iron ion sequestering activity
E	1904724	cellular_component	tertiary granule lumen
E	1904813	cellular_component	ficolin-1-rich granule lumen
F	0004322	molecular_function	ferroxidase activity
F	0005506	molecular_function	iron ion binding
F	0005515	molecular_function	protein binding
F	0005576	cellular_component	extracellular region
F	0005634	cellular_component	nucleus
F	0005737	cellular_component	cytoplasm
F	0005764	cellular_component	lysosome
F	0005776	cellular_component	autophagosome
F	0005829	cellular_component	cytosol
F	0006826	biological_process	iron ion transport
F	0006879	biological_process	intracellular iron ion homeostasis
F	0006955	biological_process	immune response
F	0008198	molecular_function	ferrous iron binding
F	0008199	molecular_function	ferric iron binding
F	0008285	biological_process	negative regulation of cell population proliferation
F	0016491	molecular_function	oxidoreductase activity
F	0031410	cellular_component	cytoplasmic vesicle
F	0042802	molecular_function	identical protein binding
F	0044754	cellular_component	autolysosome
F	0046872	molecular_function	metal ion binding
F	0048147	biological_process	negative regulation of fibroblast proliferation
F	0070062	cellular_component	extracellular exosome
F	0070288	cellular_component	ferritin complex
F	0110076	biological_process	negative regulation of ferroptosis
F	0140315	molecular_function	iron ion sequestering activity
F	1904724	cellular_component	tertiary granule lumen
F	1904813	cellular_component	ficolin-1-rich granule lumen
G	0004322	molecular_function	ferroxidase activity
G	0005506	molecular_function	iron ion binding
G	0005515	molecular_function	protein binding
G	0005576	cellular_component	extracellular region
G	0005634	cellular_component	nucleus
G	0005737	cellular_component	cytoplasm
G	0005764	cellular_component	lysosome
G	0005776	cellular_component	autophagosome
G	0005829	cellular_component	cytosol
G	0006826	biological_process	iron ion transport
G	0006879	biological_process	intracellular iron ion homeostasis
G	0006955	biological_process	immune response
G	0008198	molecular_function	ferrous iron binding
G	0008199	molecular_function	ferric iron binding
G	0008285	biological_process	negative regulation of cell population proliferation
G	0016491	molecular_function	oxidoreductase activity
G	0031410	cellular_component	cytoplasmic vesicle
G	0042802	molecular_function	identical protein binding
G	0044754	cellular_component	autolysosome
G	0046872	molecular_function	metal ion binding
G	0048147	biological_process	negative regulation of fibroblast proliferation
G	0070062	cellular_component	extracellular exosome
G	0070288	cellular_component	ferritin complex
G	0110076	biological_process	negative regulation of ferroptosis
G	0140315	molecular_function	iron ion sequestering activity
G	1904724	cellular_component	tertiary granule lumen
G	1904813	cellular_component	ficolin-1-rich granule lumen
H	0004322	molecular_function	ferroxidase activity
H	0005506	molecular_function	iron ion binding
H	0005515	molecular_function	protein binding
H	0005576	cellular_component	extracellular region
H	0005634	cellular_component	nucleus
H	0005737	cellular_component	cytoplasm
H	0005764	cellular_component	lysosome
H	0005776	cellular_component	autophagosome
H	0005829	cellular_component	cytosol
H	0006826	biological_process	iron ion transport
H	0006879	biological_process	intracellular iron ion homeostasis
H	0006955	biological_process	immune response
H	0008198	molecular_function	ferrous iron binding
H	0008199	molecular_function	ferric iron binding
H	0008285	biological_process	negative regulation of cell population proliferation
H	0016491	molecular_function	oxidoreductase activity
H	0031410	cellular_component	cytoplasmic vesicle
H	0042802	molecular_function	identical protein binding
H	0044754	cellular_component	autolysosome
H	0046872	molecular_function	metal ion binding
H	0048147	biological_process	negative regulation of fibroblast proliferation
H	0070062	cellular_component	extracellular exosome
H	0070288	cellular_component	ferritin complex
H	0110076	biological_process	negative regulation of ferroptosis
H	0140315	molecular_function	iron ion sequestering activity
H	1904724	cellular_component	tertiary granule lumen
H	1904813	cellular_component	ficolin-1-rich granule lumen

Functional Information from PDB Data

site_id	AC1
Number of Residues	6
Details	binding site for residue FE A 201

Chain	Residue
A	GLU27
A	GLU62
A	HIS65
A	HOH311
A	HOH334
A	HOH343

---

site_id	AC2
Number of Residues	5
Details	binding site for residue FE A 202

Chain	Residue
F	HIS173
G	HIS173
A	HIS173
A	CL205
C	HIS173

---

site_id	AC3
Number of Residues	5
Details	binding site for residue MG A 203

Chain	Residue
A	GLN58
A	HOH315
A	HOH347
A	HOH407
A	HOH503

---

site_id	AC4
Number of Residues	9
Details	binding site for residue MG A 204

Chain	Residue
A	ASP131
A	ASP131
A	ASP131
A	HOH329
A	HOH329
A	HOH329
A	HOH373
A	HOH373
A	HOH373

---

site_id	AC5
Number of Residues	5
Details	binding site for residue CL A 205

Chain	Residue
A	HIS173
A	FE202
C	HIS173
F	HIS173
G	HIS173

---

site_id	AC6
Number of Residues	5
Details	binding site for residue FE B 201

Chain	Residue
B	GLU27
B	GLU62
B	HIS65
B	HOH309
B	HOH334

---

site_id	AC7
Number of Residues	6
Details	binding site for residue MG B 202

Chain	Residue
B	GLN58
B	GLU61
B	HOH301
B	HOH340
B	HOH389
B	HOH484

---

site_id	AC8
Number of Residues	6
Details	binding site for residue MG B 203

Chain	Residue
B	HOH333
B	HOH491
C	HOH345
C	HOH486
D	HOH337
D	HOH480

---

site_id	AC9
Number of Residues	6
Details	binding site for residue FE C 201

Chain	Residue
C	GLU27
C	GLU62
C	HIS65
C	HOH313
C	HOH331
C	HOH344

---

site_id	AD1
Number of Residues	5
Details	binding site for residue MG C 202

Chain	Residue
C	GLN58
C	GLU61
C	HOH317
C	HOH386
C	HOH389

---

site_id	AD2
Number of Residues	9
Details	binding site for residue MG C 203

Chain	Residue
B	ASP131
B	HOH327
B	HOH330
C	ASP131
C	HOH339
D	ASP131
D	HOH308
D	HOH322
D	HOH372

---

site_id	AD3
Number of Residues	6
Details	binding site for residue MG C 204

Chain	Residue
C	ASP84
C	ASP84
C	HOH403
C	HOH403
H	HOH343
H	HOH343

---

site_id	AD4
Number of Residues	5
Details	binding site for residue FE D 201

Chain	Residue
D	GLU27
D	GLU62
D	HIS65
D	HOH312
D	HOH325

---

site_id	AD5
Number of Residues	5
Details	binding site for residue FE D 202

Chain	Residue
B	HIS173
D	HIS173
D	CL205
E	HIS173
H	HIS173

---

site_id	AD6
Number of Residues	5
Details	binding site for residue MG D 203

Chain	Residue
D	GLN58
D	HOH305
D	HOH323
D	HOH394
D	HOH497

---

site_id	AD7
Number of Residues	6
Details	binding site for residue MG D 204

Chain	Residue
D	ASP84
D	ASP84
D	HOH375
D	HOH375
D	HOH452
D	HOH452

---

site_id	AD8
Number of Residues	5
Details	binding site for residue CL D 205

Chain	Residue
B	HIS173
D	HIS173
D	FE202
E	HIS173
H	HIS173

---

site_id	AD9
Number of Residues	5
Details	binding site for residue FE E 201

Chain	Residue
E	GLU27
E	GLU62
E	HIS65
E	HOH314
E	HOH323

---

site_id	AE1
Number of Residues	5
Details	binding site for residue MG E 202

Chain	Residue
E	GLN58
E	GLU61
E	HOH302
E	HOH310
E	HOH409

---

site_id	AE2
Number of Residues	6
Details	binding site for residue MG E 203

Chain	Residue
E	HOH321
E	HOH321
E	HOH321
E	HOH484
E	HOH484
E	HOH484

---

site_id	AE3
Number of Residues	6
Details	binding site for residue MG E 204

Chain	Residue
B	HOH325
B	HOH325
E	ASP84
E	ASP84
E	HOH411
E	HOH411

---

site_id	AE4
Number of Residues	5
Details	binding site for residue FE F 201

Chain	Residue
F	GLU27
F	GLU62
F	HIS65
F	HOH308
F	HOH345

---

site_id	AE5
Number of Residues	4
Details	binding site for residue MG F 202

Chain	Residue
F	GLN58
F	HOH302
F	HOH404
F	HOH502

---

site_id	AE6
Number of Residues	6
Details	binding site for residue MG F 203

Chain	Residue
F	HOH339
F	HOH489
G	HOH331
G	HOH478
H	HOH328
H	HOH483

---

site_id	AE7
Number of Residues	6
Details	binding site for residue MG F 204

Chain	Residue
F	ASP84
F	ASP84
F	HOH334
F	HOH334
F	HOH462
F	HOH462

---

site_id	AE8
Number of Residues	5
Details	binding site for residue FE G 201

Chain	Residue
G	GLU27
G	GLU62
G	HIS65
G	HOH315
G	HOH334

---

site_id	AE9
Number of Residues	5
Details	binding site for residue MG G 202

Chain	Residue
G	GLN58
G	HOH311
G	HOH372
G	HOH397
G	HOH493

---

site_id	AF1
Number of Residues	5
Details	binding site for residue FE H 201

Chain	Residue
H	GLU27
H	GLU62
H	HIS65
H	HOH329
H	HOH333

---

site_id	AF2
Number of Residues	4
Details	binding site for residue MG H 202

Chain	Residue
H	GLN58
H	GLU61
H	HOH304
H	HOH309

---

Functional Information from PROSITE/UniProt

site_id	PS00204
Number of Residues	21
Details	FERRITIN_2 Ferritin iron-binding regions signature 2. DphLCDFIEthYLneqvkaIK

Chain	Residue	Details
A	ASP126-LYS146

---

site_id	PS00540
Number of Residues	19
Details	FERRITIN_1 Ferritin iron-binding regions signature 1. EeREhaEKLMklQNqRgGR

Chain	Residue	Details
A	GLU61-ARG79

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1192
Details	Domain: {"description":"Ferritin-like diiron","evidences":[{"source":"PROSITE-ProRule","id":"PRU00085","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	16
Details	Binding site: {"evidences":[{"source":"PubMed","id":"1992356","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"1FHA","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	24
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00085","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	8
Details	Site: {"description":"Essential for association with cargo receptor NCOA4","evidences":[{"source":"PubMed","id":"26436293","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---