5JKK
Crystal structure of the negatively supercharged variant Ftn(neg) of human heavy chain ferritin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-06-15 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.00 |
Spacegroup name | P 2 3 |
Unit cell lengths | 180.980, 180.980, 180.980 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.370 - 1.600 |
R-factor | 0.1247 |
Rwork | 0.124 |
R-free | 0.14290 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2cei |
RMSD bond length | 0.024 |
RMSD bond angle | 2.164 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.5.6) |
Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.370 | 1.700 |
High resolution limit [Å] | 1.600 | 1.600 |
Rmerge | 0.047 | 0.194 |
Number of reflections | 255788 | |
<I/σ(I)> | 40.93 | 11.21 |
Completeness [%] | 99.9 | 99.3 |
Redundancy | 13.17 | 11.16 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.52 M magnesium acetate, 100 mM Tris, pH 8.5, 8 mg/mL Ftn(neg) |