Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help

5HS3

Human thymidylate synthase complexed with dUMP and 3-amino-2-benzoyl-4-methylthieno[2,3-b]pyridin-6-ol

Functional Information from GO Data
ChainGOidnamespacecontents
A0000900molecular_functionmRNA regulatory element binding translation repressor activity
A0004799molecular_functionthymidylate synthase activity
A0005542molecular_functionfolic acid binding
A0005634cellular_componentnucleus
A0005737cellular_componentcytoplasm
A0005739cellular_componentmitochondrion
A0005743cellular_componentmitochondrial inner membrane
A0005759cellular_componentmitochondrial matrix
A0005829cellular_componentcytosol
A0006231biological_processdTMP biosynthetic process
A0006235biological_processdTTP biosynthetic process
A0008168molecular_functionmethyltransferase activity
A0009165biological_processnucleotide biosynthetic process
A0016020cellular_componentmembrane
A0016740molecular_functiontransferase activity
A0016741molecular_functiontransferase activity, transferring one-carbon groups
A0017148biological_processnegative regulation of translation
A0032259biological_processmethylation
A0035999biological_processtetrahydrofolate interconversion
A0046653biological_processtetrahydrofolate metabolic process
A0071897biological_processDNA biosynthetic process
A1990825molecular_functionsequence-specific mRNA binding
B0000900molecular_functionmRNA regulatory element binding translation repressor activity
B0004799molecular_functionthymidylate synthase activity
B0005542molecular_functionfolic acid binding
B0005634cellular_componentnucleus
B0005737cellular_componentcytoplasm
B0005739cellular_componentmitochondrion
B0005743cellular_componentmitochondrial inner membrane
B0005759cellular_componentmitochondrial matrix
B0005829cellular_componentcytosol
B0006231biological_processdTMP biosynthetic process
B0006235biological_processdTTP biosynthetic process
B0008168molecular_functionmethyltransferase activity
B0009165biological_processnucleotide biosynthetic process
B0016020cellular_componentmembrane
B0016740molecular_functiontransferase activity
B0016741molecular_functiontransferase activity, transferring one-carbon groups
B0017148biological_processnegative regulation of translation
B0032259biological_processmethylation
B0035999biological_processtetrahydrofolate interconversion
B0046653biological_processtetrahydrofolate metabolic process
B0071897biological_processDNA biosynthetic process
B1990825molecular_functionsequence-specific mRNA binding
C0000900molecular_functionmRNA regulatory element binding translation repressor activity
C0004799molecular_functionthymidylate synthase activity
C0005542molecular_functionfolic acid binding
C0005634cellular_componentnucleus
C0005737cellular_componentcytoplasm
C0005739cellular_componentmitochondrion
C0005743cellular_componentmitochondrial inner membrane
C0005759cellular_componentmitochondrial matrix
C0005829cellular_componentcytosol
C0006231biological_processdTMP biosynthetic process
C0006235biological_processdTTP biosynthetic process
C0008168molecular_functionmethyltransferase activity
C0009165biological_processnucleotide biosynthetic process
C0016020cellular_componentmembrane
C0016740molecular_functiontransferase activity
C0016741molecular_functiontransferase activity, transferring one-carbon groups
C0017148biological_processnegative regulation of translation
C0032259biological_processmethylation
C0035999biological_processtetrahydrofolate interconversion
C0046653biological_processtetrahydrofolate metabolic process
C0071897biological_processDNA biosynthetic process
C1990825molecular_functionsequence-specific mRNA binding
D0000900molecular_functionmRNA regulatory element binding translation repressor activity
D0004799molecular_functionthymidylate synthase activity
D0005542molecular_functionfolic acid binding
D0005634cellular_componentnucleus
D0005737cellular_componentcytoplasm
D0005739cellular_componentmitochondrion
D0005743cellular_componentmitochondrial inner membrane
D0005759cellular_componentmitochondrial matrix
D0005829cellular_componentcytosol
D0006231biological_processdTMP biosynthetic process
D0006235biological_processdTTP biosynthetic process
D0008168molecular_functionmethyltransferase activity
D0009165biological_processnucleotide biosynthetic process
D0016020cellular_componentmembrane
D0016740molecular_functiontransferase activity
D0016741molecular_functiontransferase activity, transferring one-carbon groups
D0017148biological_processnegative regulation of translation
D0032259biological_processmethylation
D0035999biological_processtetrahydrofolate interconversion
D0046653biological_processtetrahydrofolate metabolic process
D0071897biological_processDNA biosynthetic process
D1990825molecular_functionsequence-specific mRNA binding
E0000900molecular_functionmRNA regulatory element binding translation repressor activity
E0004799molecular_functionthymidylate synthase activity
E0005542molecular_functionfolic acid binding
E0005634cellular_componentnucleus
E0005737cellular_componentcytoplasm
E0005739cellular_componentmitochondrion
E0005743cellular_componentmitochondrial inner membrane
E0005759cellular_componentmitochondrial matrix
E0005829cellular_componentcytosol
E0006231biological_processdTMP biosynthetic process
E0006235biological_processdTTP biosynthetic process
E0008168molecular_functionmethyltransferase activity
E0009165biological_processnucleotide biosynthetic process
E0016020cellular_componentmembrane
E0016740molecular_functiontransferase activity
E0016741molecular_functiontransferase activity, transferring one-carbon groups
E0017148biological_processnegative regulation of translation
E0032259biological_processmethylation
E0035999biological_processtetrahydrofolate interconversion
E0046653biological_processtetrahydrofolate metabolic process
E0071897biological_processDNA biosynthetic process
E1990825molecular_functionsequence-specific mRNA binding
F0000900molecular_functionmRNA regulatory element binding translation repressor activity
F0004799molecular_functionthymidylate synthase activity
F0005542molecular_functionfolic acid binding
F0005634cellular_componentnucleus
F0005737cellular_componentcytoplasm
F0005739cellular_componentmitochondrion
F0005743cellular_componentmitochondrial inner membrane
F0005759cellular_componentmitochondrial matrix
F0005829cellular_componentcytosol
F0006231biological_processdTMP biosynthetic process
F0006235biological_processdTTP biosynthetic process
F0008168molecular_functionmethyltransferase activity
F0009165biological_processnucleotide biosynthetic process
F0016020cellular_componentmembrane
F0016740molecular_functiontransferase activity
F0016741molecular_functiontransferase activity, transferring one-carbon groups
F0017148biological_processnegative regulation of translation
F0032259biological_processmethylation
F0035999biological_processtetrahydrofolate interconversion
F0046653biological_processtetrahydrofolate metabolic process
F0071897biological_processDNA biosynthetic process
F1990825molecular_functionsequence-specific mRNA binding
Functional Information from PDB Data
site_idAC1
Number of Residues11
Detailsbinding site for residue UMP A 400
ChainResidue
AARG50
DARG175
DARG176
ACYS195
AARG215
ASER216
AASP218
AASN226
AHIS256
ATYR258
AKI3401

site_idAC2
Number of Residues11
Detailsbinding site for residue KI3 A 401
ChainResidue
AARG50
AGLU87
AILE108
ATRP109
AASN112
AASP218
AGLY222
APHE225
AALA312
AVAL313
AUMP400

site_idAC3
Number of Residues12
Detailsbinding site for residue UMP B 400
ChainResidue
BARG50
BCYS195
BHIS196
BARG215
BSER216
BASP218
BASN226
BHIS256
BTYR258
BKI3401
EARG175
EARG176

site_idAC4
Number of Residues11
Detailsbinding site for residue KI3 B 401
ChainResidue
BARG50
BGLU87
BILE108
BTRP109
BASN112
BASP218
BGLY222
BPHE225
BMET311
BALA312
BUMP400

site_idAC5
Number of Residues13
Detailsbinding site for residue UMP C 400
ChainResidue
CARG50
CCYS195
CHIS196
CGLN214
CARG215
CSER216
CGLY217
CASP218
CASN226
CHIS256
CTYR258
FARG175
FARG176

site_idAC6
Number of Residues12
Detailsbinding site for residue UMP D 400
ChainResidue
AARG175
AARG176
DCYS195
DHIS196
DGLN214
DARG215
DSER216
DGLY217
DASP218
DASN226
DHIS256
DTYR258

site_idAC7
Number of Residues13
Detailsbinding site for residue UMP E 400
ChainResidue
BARG175
BARG176
EARG50
ECYS195
EHIS196
EGLN214
EARG215
ESER216
EGLY217
EASN226
EHIS256
ETYR258
EKI3401

site_idAC8
Number of Residues9
Detailsbinding site for residue KI3 E 401
ChainResidue
EARG50
EGLU87
EILE108
ETRP109
EASN112
EGLY222
EPHE225
EASN226
EUMP400

site_idAC9
Number of Residues11
Detailsbinding site for residue UMP F 400
ChainResidue
FASP218
FASN226
FHIS256
FTYR258
CARG175
CARG176
FCYS195
FHIS196
FGLN214
FARG215
FSER216

Functional Information from PROSITE/UniProt
site_idPS00091
Number of Residues29
DetailsTHYMIDYLATE_SYNTHASE Thymidylate synthase active site. RriImcaWNprdlplma.....LpPCHalcQFyV
ChainResidueDetails
AARG175-VAL203

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues6
DetailsACT_SITE: Nucleophile => ECO:0000250|UniProtKB:P0A884
ChainResidueDetails
ACYS195
BCYS195
CCYS195
DCYS195
ECYS195
FCYS195

site_idSWS_FT_FI2
Number of Residues12
DetailsBINDING: in other chain => ECO:0000250|UniProtKB:P0A884
ChainResidueDetails
AARG50
EASN226
FARG50
FASN226
AASN226
BARG50
BASN226
CARG50
CASN226
DARG50
DASN226
EARG50

site_idSWS_FT_FI3
Number of Residues6
DetailsBINDING: BINDING => ECO:0000250|UniProtKB:P45352
ChainResidueDetails
AARG175
BARG175
CARG175
DARG175
EARG175
FARG175

site_idSWS_FT_FI4
Number of Residues18
DetailsBINDING: in other chain => ECO:0000250|UniProtKB:P45352
ChainResidueDetails
ACYS195
DCYS195
DARG215
DHIS256
ECYS195
EARG215
EHIS256
FCYS195
FARG215
FHIS256
AARG215
AHIS256
BCYS195
BARG215
BHIS256
CCYS195
CARG215
CHIS256

site_idSWS_FT_FI5
Number of Residues12
DetailsBINDING: BINDING => ECO:0000250|UniProtKB:P0A884
ChainResidueDetails
AASP218
EALA312
FASP218
FALA312
AALA312
BASP218
BALA312
CASP218
CALA312
DASP218
DALA312
EASP218

site_idSWS_FT_FI6
Number of Residues6
DetailsMOD_RES: Phosphoserine => ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:23186163
ChainResidueDetails
ASER114
BSER114
CSER114
DSER114
ESER114
FSER114

site_idSWS_FT_FI7
Number of Residues24
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733
ChainResidueDetails
ALYS287
CLYS292
CLYS308
DLYS287
DLYS292
DLYS308
ELYS287
ELYS292
ELYS308
FLYS287
FLYS292
FLYS308
ALYS292
ALYS308
BLYS287
BLYS292
BLYS308
CLYS287

238268

PDB entries from 2025-07-02

PDB statisticsPDBj update infoContact PDBjnumon