5HI6
The high resolution structure of dihydrofolate reductase from Yersinia pestis complex with methotrexate as closed form
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0004146 | molecular_function | dihydrofolate reductase activity |
| A | 0005829 | cellular_component | cytosol |
| A | 0006730 | biological_process | one-carbon metabolic process |
| A | 0016491 | molecular_function | oxidoreductase activity |
| A | 0044281 | biological_process | small molecule metabolic process |
| A | 0046452 | biological_process | dihydrofolate metabolic process |
| A | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| A | 0046655 | biological_process | folic acid metabolic process |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0050661 | molecular_function | NADP binding |
| A | 0070401 | molecular_function | NADP+ binding |
| B | 0004146 | molecular_function | dihydrofolate reductase activity |
| B | 0005829 | cellular_component | cytosol |
| B | 0006730 | biological_process | one-carbon metabolic process |
| B | 0016491 | molecular_function | oxidoreductase activity |
| B | 0044281 | biological_process | small molecule metabolic process |
| B | 0046452 | biological_process | dihydrofolate metabolic process |
| B | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
| B | 0046655 | biological_process | folic acid metabolic process |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0050661 | molecular_function | NADP binding |
| B | 0070401 | molecular_function | NADP+ binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 15 |
| Details | binding site for residue MTX A 201 |
| Chain | Residue |
| A | ILE6 |
| A | TYR101 |
| A | THR114 |
| A | HOH308 |
| A | HOH321 |
| A | HOH335 |
| A | HOH362 |
| A | ALA7 |
| A | ASP28 |
| A | PHE32 |
| A | LYS33 |
| A | ARG53 |
| A | LEU55 |
| A | ARG58 |
| A | MSE95 |
| site_id | AC2 |
| Number of Residues | 7 |
| Details | binding site for residue CA A 202 |
| Chain | Residue |
| A | MSE17 |
| A | GLU18 |
| A | HOH318 |
| A | HOH353 |
| A | HOH371 |
| A | HOH426 |
| A | HOH429 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | binding site for residue K A 203 |
| Chain | Residue |
| A | GLU71 |
| B | ASP12 |
| B | GLU119 |
| site_id | AC4 |
| Number of Residues | 17 |
| Details | binding site for residue MTX B 201 |
| Chain | Residue |
| B | ILE6 |
| B | ALA7 |
| B | ALA8 |
| B | ASP28 |
| B | LEU29 |
| B | PHE32 |
| B | LYS33 |
| B | SER50 |
| B | ARG53 |
| B | ARG58 |
| B | MSE95 |
| B | TYR101 |
| B | THR114 |
| B | HOH303 |
| B | HOH336 |
| B | HOH351 |
| B | HOH362 |
| site_id | AC5 |
| Number of Residues | 4 |
| Details | binding site for residue K B 202 |
| Chain | Residue |
| B | ALA8 |
| B | ILE15 |
| B | HOH353 |
| B | HOH424 |
| site_id | AC6 |
| Number of Residues | 3 |
| Details | binding site for residue CL B 203 |
| Chain | Residue |
| B | GLU130 |
| B | GLU133 |
| B | TRP134 |
| site_id | AC7 |
| Number of Residues | 3 |
| Details | binding site for residue CL B 204 |
| Chain | Residue |
| B | GLY44 |
| B | THR47 |
| B | GLY97 |
Functional Information from PROSITE/UniProt
| site_id | PS00075 |
| Number of Residues | 23 |
| Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. VIGmenaMPWhlpa.DlawFkrnT |
| Chain | Residue | Details |
| A | VAL14-THR36 |
