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5FKU

cryo-EM structure of the E. coli replicative DNA polymerase complex in DNA free state (DNA polymerase III alpha, beta, epsilon, tau complex)

Functional Information from GO Data
ChainGOidnamespacecontents
A0003676molecular_functionnucleic acid binding
A0003677molecular_functionDNA binding
A0003824molecular_functioncatalytic activity
A0003887molecular_functionDNA-directed DNA polymerase activity
A0005515molecular_functionprotein binding
A0005737cellular_componentcytoplasm
A0005829cellular_componentcytosol
A0006260biological_processDNA replication
A0006261biological_processDNA-templated DNA replication
A0006272biological_processleading strand elongation
A0006273biological_processlagging strand elongation
A0008408molecular_function3'-5' exonuclease activity
A0009360cellular_componentDNA polymerase III complex
A0030894cellular_componentreplisome
A0034061molecular_functionDNA polymerase activity
A0044776cellular_componentDNA polymerase III, core complex
A0071897biological_processDNA biosynthetic process
A0140640molecular_functioncatalytic activity, acting on a nucleic acid
B0003677molecular_functionDNA binding
B0003887molecular_functionDNA-directed DNA polymerase activity
B0005515molecular_functionprotein binding
B0005737cellular_componentcytoplasm
B0005829cellular_componentcytosol
B0006260biological_processDNA replication
B0006261biological_processDNA-templated DNA replication
B0006271biological_processDNA strand elongation involved in DNA replication
B0006974biological_processDNA damage response
B0008408molecular_function3'-5' exonuclease activity
B0009360cellular_componentDNA polymerase III complex
B0030174biological_processregulation of DNA-templated DNA replication initiation
B0030894cellular_componentreplisome
B0032297biological_processnegative regulation of DNA-templated DNA replication initiation
B0042276biological_processerror-prone translesion synthesis
B0042802molecular_functionidentical protein binding
B0042803molecular_functionprotein homodimerization activity
B0044787biological_processbacterial-type DNA replication
B1990078cellular_componentreplication inhibiting complex
B1990085cellular_componentHda-beta clamp complex
C0003677molecular_functionDNA binding
C0003887molecular_functionDNA-directed DNA polymerase activity
C0005515molecular_functionprotein binding
C0005737cellular_componentcytoplasm
C0005829cellular_componentcytosol
C0006260biological_processDNA replication
C0006261biological_processDNA-templated DNA replication
C0006271biological_processDNA strand elongation involved in DNA replication
C0006974biological_processDNA damage response
C0008408molecular_function3'-5' exonuclease activity
C0009360cellular_componentDNA polymerase III complex
C0030174biological_processregulation of DNA-templated DNA replication initiation
C0030894cellular_componentreplisome
C0032297biological_processnegative regulation of DNA-templated DNA replication initiation
C0042276biological_processerror-prone translesion synthesis
C0042802molecular_functionidentical protein binding
C0042803molecular_functionprotein homodimerization activity
C0044787biological_processbacterial-type DNA replication
C1990078cellular_componentreplication inhibiting complex
C1990085cellular_componentHda-beta clamp complex
D0003676molecular_functionnucleic acid binding
D0003677molecular_functionDNA binding
D0003887molecular_functionDNA-directed DNA polymerase activity
D0004527molecular_functionexonuclease activity
D0005515molecular_functionprotein binding
D0005829cellular_componentcytosol
D0006259biological_processDNA metabolic process
D0006260biological_processDNA replication
D0006261biological_processDNA-templated DNA replication
D0006272biological_processleading strand elongation
D0006273biological_processlagging strand elongation
D0008408molecular_function3'-5' exonuclease activity
D0009360cellular_componentDNA polymerase III complex
D0030894cellular_componentreplisome
D0034061molecular_functionDNA polymerase activity
D0044776cellular_componentDNA polymerase III, core complex
D0045004biological_processDNA replication proofreading
D0046872molecular_functionmetal ion binding
D0071897biological_processDNA biosynthetic process
E0003887molecular_functionDNA-directed DNA polymerase activity
Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues1
DetailsACT_SITE: Proton acceptor
ChainResidueDetails
DHIS162
BGLN149
BTYR153
CARG24
CGLN149
CTYR153

site_idSWS_FT_FI2
Number of Residues5
DetailsBINDING:
ChainResidueDetails
DASP12
DGLU14
DGLU61
DHIS66
DASP167

Catalytic Information from CSA
site_idMCSA1
Number of Residues5
DetailsM-CSA 754
ChainResidueDetails
DASP12metal ligand
DGLU14hydrogen bond acceptor, metal ligand, proton acceptor, proton donor
DGLU61electrostatic stabiliser, increase basicity, polar interaction
DHIS162proton acceptor, proton donor
DASP167metal ligand

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PDB entries from 2024-11-06

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