5FKU
cryo-EM structure of the E. coli replicative DNA polymerase complex in DNA free state (DNA polymerase III alpha, beta, epsilon, tau complex)
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK III |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K2 QUANTUM (4k x 4k) |
| Number of Images | 1350 |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 8.34 |
| Symmetry | C1 |
