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5FCT

Mouse thymidylate synthase in ternary complex with FdUMP and methylenetetrahydrofolate.

Functional Information from GO Data
ChainGOidnamespacecontents
A0000900molecular_functionmRNA regulatory element binding translation repressor activity
A0003729molecular_functionmRNA binding
A0004799molecular_functionthymidylate synthase activity
A0005542molecular_functionfolic acid binding
A0005634cellular_componentnucleus
A0005737cellular_componentcytoplasm
A0005739cellular_componentmitochondrion
A0005743cellular_componentmitochondrial inner membrane
A0005759cellular_componentmitochondrial matrix
A0006206biological_processpyrimidine nucleobase metabolic process
A0006231biological_processdTMP biosynthetic process
A0006235biological_processdTTP biosynthetic process
A0006417biological_processregulation of translation
A0007623biological_processcircadian rhythm
A0008168molecular_functionmethyltransferase activity
A0009165biological_processnucleotide biosynthetic process
A0009410biological_processresponse to xenobiotic stimulus
A0009636biological_processresponse to toxic substance
A0014070biological_processresponse to organic cyclic compound
A0016741molecular_functiontransferase activity, transferring one-carbon groups
A0017148biological_processnegative regulation of translation
A0019860biological_processuracil metabolic process
A0032259biological_processmethylation
A0032570biological_processresponse to progesterone
A0033189biological_processresponse to vitamin A
A0034097biological_processresponse to cytokine
A0035999biological_processtetrahydrofolate interconversion
A0042803molecular_functionprotein homodimerization activity
A0045471biological_processresponse to ethanol
A0046653biological_processtetrahydrofolate metabolic process
A0046683biological_processresponse to organophosphorus
A0048589biological_processdevelopmental growth
A0051216biological_processcartilage development
A0051384biological_processresponse to glucocorticoid
A0051593biological_processresponse to folic acid
A0060574biological_processintestinal epithelial cell maturation
A0097421biological_processliver regeneration
A1901363molecular_functionheterocyclic compound binding
A1990825molecular_functionsequence-specific mRNA binding
B0000900molecular_functionmRNA regulatory element binding translation repressor activity
B0003729molecular_functionmRNA binding
B0004799molecular_functionthymidylate synthase activity
B0005542molecular_functionfolic acid binding
B0005634cellular_componentnucleus
B0005737cellular_componentcytoplasm
B0005739cellular_componentmitochondrion
B0005743cellular_componentmitochondrial inner membrane
B0005759cellular_componentmitochondrial matrix
B0006206biological_processpyrimidine nucleobase metabolic process
B0006231biological_processdTMP biosynthetic process
B0006235biological_processdTTP biosynthetic process
B0006417biological_processregulation of translation
B0007623biological_processcircadian rhythm
B0008168molecular_functionmethyltransferase activity
B0009165biological_processnucleotide biosynthetic process
B0009410biological_processresponse to xenobiotic stimulus
B0009636biological_processresponse to toxic substance
B0014070biological_processresponse to organic cyclic compound
B0016741molecular_functiontransferase activity, transferring one-carbon groups
B0017148biological_processnegative regulation of translation
B0019860biological_processuracil metabolic process
B0032259biological_processmethylation
B0032570biological_processresponse to progesterone
B0033189biological_processresponse to vitamin A
B0034097biological_processresponse to cytokine
B0035999biological_processtetrahydrofolate interconversion
B0042803molecular_functionprotein homodimerization activity
B0045471biological_processresponse to ethanol
B0046653biological_processtetrahydrofolate metabolic process
B0046683biological_processresponse to organophosphorus
B0048589biological_processdevelopmental growth
B0051216biological_processcartilage development
B0051384biological_processresponse to glucocorticoid
B0051593biological_processresponse to folic acid
B0060574biological_processintestinal epithelial cell maturation
B0097421biological_processliver regeneration
B1901363molecular_functionheterocyclic compound binding
B1990825molecular_functionsequence-specific mRNA binding
Functional Information from PDB Data
site_idAC1
Number of Residues19
Detailsbinding site for residue UFP A 401
ChainResidue
AARG44
AASP212
AASN220
AHIS250
ATYR252
AC2F402
AHOH569
AHOH675
AHOH708
BARG169
BARG170
ATRP103
ATYR129
ACYS189
AHIS190
AGLN208
AARG209
ASER210
AGLY211

site_idAC2
Number of Residues22
Detailsbinding site for residue C2F A 402
ChainResidue
APHE74
AILE102
ATRP103
AASN106
ALEU186
AASP212
ALEU215
AGLY216
AMET305
AALA306
AUFP401
AHOH506
AHOH514
AHOH558
AHOH575
AHOH590
AHOH636
AHOH644
AHOH647
AHOH667
AHOH670
AHOH701

site_idAC3
Number of Residues41
Detailsbinding site for residues UFP B 401 and C2F B 402
ChainResidue
AARG169
AARG170
BARG44
BLYS71
BARG72
BVAL73
BPHE74
BILE102
BTRP103
BASN106
BTYR129
BLEU186
BCYS189
BHIS190
BGLN208
BARG209
BSER210
BGLY211
BASP212
BLEU215
BGLY216
BPHE219
BASN220
BHIS250
BTYR252
BILE301
BMET303
BMET305
BALA306
BHOH505
BHOH512
BHOH534
BHOH561
BHOH601
BHOH608
BHOH610
BHOH616
BHOH618
BHOH619
BHOH626
BHOH672

site_idAC4
Number of Residues41
Detailsbinding site for residues UFP B 401 and C2F B 402
ChainResidue
BGLY211
BASP212
BLEU215
BGLY216
BPHE219
BASN220
BHIS250
BTYR252
BILE301
BMET303
BMET305
BALA306
BHOH505
BHOH512
BHOH534
BHOH561
BHOH601
BHOH608
BHOH610
BHOH616
BHOH618
BHOH619
BHOH626
BHOH672
AARG169
AARG170
BARG44
BLYS71
BARG72
BVAL73
BPHE74
BILE102
BTRP103
BASN106
BTYR129
BLEU186
BCYS189
BHIS190
BGLN208
BARG209
BSER210

site_idAC5
Number of Residues21
Detailsbinding site for Di-peptide UFP B 401 and CYS B 189
ChainResidue
AARG169
AARG170
BARG44
BTRP103
BTYR129
BPRO187
BPRO188
BHIS190
BALA191
BGLN208
BARG209
BSER210
BGLY211
BASP212
BASN220
BHIS250
BTYR252
BC2F402
BHOH601
BHOH616
BHOH672

Functional Information from PROSITE/UniProt
site_idPS00091
Number of Residues29
DetailsTHYMIDYLATE_SYNTHASE Thymidylate synthase active site. RriImcaWNpkdlplma.....LpPCHalcQFyV
ChainResidueDetails
AARG169-VAL197

Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues2
DetailsACT_SITE: Nucleophile => ECO:0000250|UniProtKB:P0A884
ChainResidueDetails
ACYS189
BCYS189

site_idSWS_FT_FI2
Number of Residues4
DetailsBINDING: in other chain => ECO:0000250|UniProtKB:P0A884
ChainResidueDetails
AARG44
AASN220
BARG44
BASN220

site_idSWS_FT_FI3
Number of Residues2
DetailsBINDING: BINDING => ECO:0000250|UniProtKB:P45352
ChainResidueDetails
AARG169
BARG169

site_idSWS_FT_FI4
Number of Residues6
DetailsBINDING: in other chain => ECO:0000250|UniProtKB:P45352
ChainResidueDetails
ACYS189
AARG209
AHIS250
BCYS189
BARG209
BHIS250

site_idSWS_FT_FI5
Number of Residues4
DetailsBINDING: BINDING => ECO:0000250|UniProtKB:P0A884
ChainResidueDetails
AASP212
AALA306
BASP212
BALA306

site_idSWS_FT_FI6
Number of Residues2
DetailsMOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P04818
ChainResidueDetails
ASER108
BSER108

site_idSWS_FT_FI7
Number of Residues6
DetailsCROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0000250|UniProtKB:P04818
ChainResidueDetails
ALYS286
ALYS302
BLYS286
BLYS302

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PDB entries from 2024-11-06

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