5FCT
Mouse thymidylate synthase in ternary complex with FdUMP and methylenetetrahydrofolate.
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000900 | molecular_function | mRNA regulatory element binding translation repressor activity |
| A | 0003729 | molecular_function | mRNA binding |
| A | 0004799 | molecular_function | thymidylate synthase activity |
| A | 0005542 | molecular_function | folic acid binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005657 | cellular_component | replication fork |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005743 | cellular_component | mitochondrial inner membrane |
| A | 0005759 | cellular_component | mitochondrial matrix |
| A | 0005829 | cellular_component | cytosol |
| A | 0006207 | biological_process | 'de novo' pyrimidine nucleobase biosynthetic process |
| A | 0006231 | biological_process | dTMP biosynthetic process |
| A | 0006235 | biological_process | dTTP biosynthetic process |
| A | 0006260 | biological_process | DNA replication |
| A | 0006417 | biological_process | regulation of translation |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0009165 | biological_process | nucleotide biosynthetic process |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| A | 0017148 | biological_process | negative regulation of translation |
| A | 0032259 | biological_process | methylation |
| A | 0035999 | biological_process | tetrahydrofolate interconversion |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0046653 | biological_process | tetrahydrofolate metabolic process |
| A | 1901363 | molecular_function | heterocyclic compound binding |
| A | 1990825 | molecular_function | sequence-specific mRNA binding |
| B | 0000900 | molecular_function | mRNA regulatory element binding translation repressor activity |
| B | 0003729 | molecular_function | mRNA binding |
| B | 0004799 | molecular_function | thymidylate synthase activity |
| B | 0005542 | molecular_function | folic acid binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005657 | cellular_component | replication fork |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005739 | cellular_component | mitochondrion |
| B | 0005743 | cellular_component | mitochondrial inner membrane |
| B | 0005759 | cellular_component | mitochondrial matrix |
| B | 0005829 | cellular_component | cytosol |
| B | 0006207 | biological_process | 'de novo' pyrimidine nucleobase biosynthetic process |
| B | 0006231 | biological_process | dTMP biosynthetic process |
| B | 0006235 | biological_process | dTTP biosynthetic process |
| B | 0006260 | biological_process | DNA replication |
| B | 0006417 | biological_process | regulation of translation |
| B | 0008168 | molecular_function | methyltransferase activity |
| B | 0009165 | biological_process | nucleotide biosynthetic process |
| B | 0016740 | molecular_function | transferase activity |
| B | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| B | 0017148 | biological_process | negative regulation of translation |
| B | 0032259 | biological_process | methylation |
| B | 0035999 | biological_process | tetrahydrofolate interconversion |
| B | 0042803 | molecular_function | protein homodimerization activity |
| B | 0046653 | biological_process | tetrahydrofolate metabolic process |
| B | 1901363 | molecular_function | heterocyclic compound binding |
| B | 1990825 | molecular_function | sequence-specific mRNA binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 19 |
| Details | binding site for residue UFP A 401 |
| Chain | Residue |
| A | ARG44 |
| A | ASP212 |
| A | ASN220 |
| A | HIS250 |
| A | TYR252 |
| A | C2F402 |
| A | HOH569 |
| A | HOH675 |
| A | HOH708 |
| B | ARG169 |
| B | ARG170 |
| A | TRP103 |
| A | TYR129 |
| A | CYS189 |
| A | HIS190 |
| A | GLN208 |
| A | ARG209 |
| A | SER210 |
| A | GLY211 |
| site_id | AC2 |
| Number of Residues | 22 |
| Details | binding site for residue C2F A 402 |
| Chain | Residue |
| A | PHE74 |
| A | ILE102 |
| A | TRP103 |
| A | ASN106 |
| A | LEU186 |
| A | ASP212 |
| A | LEU215 |
| A | GLY216 |
| A | MET305 |
| A | ALA306 |
| A | UFP401 |
| A | HOH506 |
| A | HOH514 |
| A | HOH558 |
| A | HOH575 |
| A | HOH590 |
| A | HOH636 |
| A | HOH644 |
| A | HOH647 |
| A | HOH667 |
| A | HOH670 |
| A | HOH701 |
| site_id | AC3 |
| Number of Residues | 41 |
| Details | binding site for residues UFP B 401 and C2F B 402 |
| Chain | Residue |
| A | ARG169 |
| A | ARG170 |
| B | ARG44 |
| B | LYS71 |
| B | ARG72 |
| B | VAL73 |
| B | PHE74 |
| B | ILE102 |
| B | TRP103 |
| B | ASN106 |
| B | TYR129 |
| B | LEU186 |
| B | CYS189 |
| B | HIS190 |
| B | GLN208 |
| B | ARG209 |
| B | SER210 |
| B | GLY211 |
| B | ASP212 |
| B | LEU215 |
| B | GLY216 |
| B | PHE219 |
| B | ASN220 |
| B | HIS250 |
| B | TYR252 |
| B | ILE301 |
| B | MET303 |
| B | MET305 |
| B | ALA306 |
| B | HOH505 |
| B | HOH512 |
| B | HOH534 |
| B | HOH561 |
| B | HOH601 |
| B | HOH608 |
| B | HOH610 |
| B | HOH616 |
| B | HOH618 |
| B | HOH619 |
| B | HOH626 |
| B | HOH672 |
| site_id | AC4 |
| Number of Residues | 41 |
| Details | binding site for residues UFP B 401 and C2F B 402 |
| Chain | Residue |
| B | GLY211 |
| B | ASP212 |
| B | LEU215 |
| B | GLY216 |
| B | PHE219 |
| B | ASN220 |
| B | HIS250 |
| B | TYR252 |
| B | ILE301 |
| B | MET303 |
| B | MET305 |
| B | ALA306 |
| B | HOH505 |
| B | HOH512 |
| B | HOH534 |
| B | HOH561 |
| B | HOH601 |
| B | HOH608 |
| B | HOH610 |
| B | HOH616 |
| B | HOH618 |
| B | HOH619 |
| B | HOH626 |
| B | HOH672 |
| A | ARG169 |
| A | ARG170 |
| B | ARG44 |
| B | LYS71 |
| B | ARG72 |
| B | VAL73 |
| B | PHE74 |
| B | ILE102 |
| B | TRP103 |
| B | ASN106 |
| B | TYR129 |
| B | LEU186 |
| B | CYS189 |
| B | HIS190 |
| B | GLN208 |
| B | ARG209 |
| B | SER210 |
| site_id | AC5 |
| Number of Residues | 21 |
| Details | binding site for Di-peptide UFP B 401 and CYS B 189 |
| Chain | Residue |
| A | ARG169 |
| A | ARG170 |
| B | ARG44 |
| B | TRP103 |
| B | TYR129 |
| B | PRO187 |
| B | PRO188 |
| B | HIS190 |
| B | ALA191 |
| B | GLN208 |
| B | ARG209 |
| B | SER210 |
| B | GLY211 |
| B | ASP212 |
| B | ASN220 |
| B | HIS250 |
| B | TYR252 |
| B | C2F402 |
| B | HOH601 |
| B | HOH616 |
| B | HOH672 |
Functional Information from PROSITE/UniProt
| site_id | PS00091 |
| Number of Residues | 29 |
| Details | THYMIDYLATE_SYNTHASE Thymidylate synthase active site. RriImcaWNpkdlplma.....LpPCHalcQFyV |
| Chain | Residue | Details |
| A | ARG169-VAL197 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI2 |
| Number of Residues | 4 |
| Details | Binding site: {"description":"in other chain","evidences":[{"source":"UniProtKB","id":"P0A884","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"P45352","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 12 |
| Details | Binding site: {"description":"in other chain","evidences":[{"source":"UniProtKB","id":"P45352","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 4 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"P0A884","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P04818","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 6 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"UniProtKB","id":"P04818","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
