5EU9

Structure of Human Enolase 2 in complex with ((3S,5S)-1,5-dihydroxy-3-methyl-2-oxopyrrolidin-3-yl)phosphonic acid

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000015	cellular_component	phosphopyruvate hydratase complex
A	0000287	molecular_function	magnesium ion binding
A	0001917	cellular_component	photoreceptor inner segment
A	0004634	molecular_function	phosphopyruvate hydratase activity
A	0005515	molecular_function	protein binding
A	0005615	cellular_component	extracellular space
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0005938	cellular_component	cell cortex
A	0006094	biological_process	gluconeogenesis
A	0006096	biological_process	glycolytic process
A	0009410	biological_process	response to xenobiotic stimulus
A	0009986	cellular_component	cell surface
A	0016020	cellular_component	membrane
A	0016829	molecular_function	lyase activity
A	0019899	molecular_function	enzyme binding
A	0030426	cellular_component	growth cone
A	0032355	biological_process	response to estradiol
A	0042802	molecular_function	identical protein binding
A	0043025	cellular_component	neuronal cell body
A	0043204	cellular_component	perikaryon
A	0044877	molecular_function	protein-containing complex binding
A	0045121	cellular_component	membrane raft
A	0046872	molecular_function	metal ion binding
A	0061621	biological_process	canonical glycolysis
A	0070062	cellular_component	extracellular exosome
A	0097060	cellular_component	synaptic membrane
B	0000015	cellular_component	phosphopyruvate hydratase complex
B	0000287	molecular_function	magnesium ion binding
B	0001917	cellular_component	photoreceptor inner segment
B	0004634	molecular_function	phosphopyruvate hydratase activity
B	0005515	molecular_function	protein binding
B	0005615	cellular_component	extracellular space
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0005938	cellular_component	cell cortex
B	0006094	biological_process	gluconeogenesis
B	0006096	biological_process	glycolytic process
B	0009410	biological_process	response to xenobiotic stimulus
B	0009986	cellular_component	cell surface
B	0016020	cellular_component	membrane
B	0016829	molecular_function	lyase activity
B	0019899	molecular_function	enzyme binding
B	0030426	cellular_component	growth cone
B	0032355	biological_process	response to estradiol
B	0042802	molecular_function	identical protein binding
B	0043025	cellular_component	neuronal cell body
B	0043204	cellular_component	perikaryon
B	0044877	molecular_function	protein-containing complex binding
B	0045121	cellular_component	membrane raft
B	0046872	molecular_function	metal ion binding
B	0061621	biological_process	canonical glycolysis
B	0070062	cellular_component	extracellular exosome
B	0097060	cellular_component	synaptic membrane
C	0000015	cellular_component	phosphopyruvate hydratase complex
C	0000287	molecular_function	magnesium ion binding
C	0001917	cellular_component	photoreceptor inner segment
C	0004634	molecular_function	phosphopyruvate hydratase activity
C	0005515	molecular_function	protein binding
C	0005615	cellular_component	extracellular space
C	0005737	cellular_component	cytoplasm
C	0005829	cellular_component	cytosol
C	0005886	cellular_component	plasma membrane
C	0005938	cellular_component	cell cortex
C	0006094	biological_process	gluconeogenesis
C	0006096	biological_process	glycolytic process
C	0009410	biological_process	response to xenobiotic stimulus
C	0009986	cellular_component	cell surface
C	0016020	cellular_component	membrane
C	0016829	molecular_function	lyase activity
C	0019899	molecular_function	enzyme binding
C	0030426	cellular_component	growth cone
C	0032355	biological_process	response to estradiol
C	0042802	molecular_function	identical protein binding
C	0043025	cellular_component	neuronal cell body
C	0043204	cellular_component	perikaryon
C	0044877	molecular_function	protein-containing complex binding
C	0045121	cellular_component	membrane raft
C	0046872	molecular_function	metal ion binding
C	0061621	biological_process	canonical glycolysis
C	0070062	cellular_component	extracellular exosome
C	0097060	cellular_component	synaptic membrane
D	0000015	cellular_component	phosphopyruvate hydratase complex
D	0000287	molecular_function	magnesium ion binding
D	0001917	cellular_component	photoreceptor inner segment
D	0004634	molecular_function	phosphopyruvate hydratase activity
D	0005515	molecular_function	protein binding
D	0005615	cellular_component	extracellular space
D	0005737	cellular_component	cytoplasm
D	0005829	cellular_component	cytosol
D	0005886	cellular_component	plasma membrane
D	0005938	cellular_component	cell cortex
D	0006094	biological_process	gluconeogenesis
D	0006096	biological_process	glycolytic process
D	0009410	biological_process	response to xenobiotic stimulus
D	0009986	cellular_component	cell surface
D	0016020	cellular_component	membrane
D	0016829	molecular_function	lyase activity
D	0019899	molecular_function	enzyme binding
D	0030426	cellular_component	growth cone
D	0032355	biological_process	response to estradiol
D	0042802	molecular_function	identical protein binding
D	0043025	cellular_component	neuronal cell body
D	0043204	cellular_component	perikaryon
D	0044877	molecular_function	protein-containing complex binding
D	0045121	cellular_component	membrane raft
D	0046872	molecular_function	metal ion binding
D	0061621	biological_process	canonical glycolysis
D	0070062	cellular_component	extracellular exosome
D	0097060	cellular_component	synaptic membrane
E	0000015	cellular_component	phosphopyruvate hydratase complex
E	0000287	molecular_function	magnesium ion binding
E	0001917	cellular_component	photoreceptor inner segment
E	0004634	molecular_function	phosphopyruvate hydratase activity
E	0005515	molecular_function	protein binding
E	0005615	cellular_component	extracellular space
E	0005737	cellular_component	cytoplasm
E	0005829	cellular_component	cytosol
E	0005886	cellular_component	plasma membrane
E	0005938	cellular_component	cell cortex
E	0006094	biological_process	gluconeogenesis
E	0006096	biological_process	glycolytic process
E	0009410	biological_process	response to xenobiotic stimulus
E	0009986	cellular_component	cell surface
E	0016020	cellular_component	membrane
E	0016829	molecular_function	lyase activity
E	0019899	molecular_function	enzyme binding
E	0030426	cellular_component	growth cone
E	0032355	biological_process	response to estradiol
E	0042802	molecular_function	identical protein binding
E	0043025	cellular_component	neuronal cell body
E	0043204	cellular_component	perikaryon
E	0044877	molecular_function	protein-containing complex binding
E	0045121	cellular_component	membrane raft
E	0046872	molecular_function	metal ion binding
E	0061621	biological_process	canonical glycolysis
E	0070062	cellular_component	extracellular exosome
E	0097060	cellular_component	synaptic membrane
F	0000015	cellular_component	phosphopyruvate hydratase complex
F	0000287	molecular_function	magnesium ion binding
F	0001917	cellular_component	photoreceptor inner segment
F	0004634	molecular_function	phosphopyruvate hydratase activity
F	0005515	molecular_function	protein binding
F	0005615	cellular_component	extracellular space
F	0005737	cellular_component	cytoplasm
F	0005829	cellular_component	cytosol
F	0005886	cellular_component	plasma membrane
F	0005938	cellular_component	cell cortex
F	0006094	biological_process	gluconeogenesis
F	0006096	biological_process	glycolytic process
F	0009410	biological_process	response to xenobiotic stimulus
F	0009986	cellular_component	cell surface
F	0016020	cellular_component	membrane
F	0016829	molecular_function	lyase activity
F	0019899	molecular_function	enzyme binding
F	0030426	cellular_component	growth cone
F	0032355	biological_process	response to estradiol
F	0042802	molecular_function	identical protein binding
F	0043025	cellular_component	neuronal cell body
F	0043204	cellular_component	perikaryon
F	0044877	molecular_function	protein-containing complex binding
F	0045121	cellular_component	membrane raft
F	0046872	molecular_function	metal ion binding
F	0061621	biological_process	canonical glycolysis
F	0070062	cellular_component	extracellular exosome
F	0097060	cellular_component	synaptic membrane
G	0000015	cellular_component	phosphopyruvate hydratase complex
G	0000287	molecular_function	magnesium ion binding
G	0001917	cellular_component	photoreceptor inner segment
G	0004634	molecular_function	phosphopyruvate hydratase activity
G	0005515	molecular_function	protein binding
G	0005615	cellular_component	extracellular space
G	0005737	cellular_component	cytoplasm
G	0005829	cellular_component	cytosol
G	0005886	cellular_component	plasma membrane
G	0005938	cellular_component	cell cortex
G	0006094	biological_process	gluconeogenesis
G	0006096	biological_process	glycolytic process
G	0009410	biological_process	response to xenobiotic stimulus
G	0009986	cellular_component	cell surface
G	0016020	cellular_component	membrane
G	0016829	molecular_function	lyase activity
G	0019899	molecular_function	enzyme binding
G	0030426	cellular_component	growth cone
G	0032355	biological_process	response to estradiol
G	0042802	molecular_function	identical protein binding
G	0043025	cellular_component	neuronal cell body
G	0043204	cellular_component	perikaryon
G	0044877	molecular_function	protein-containing complex binding
G	0045121	cellular_component	membrane raft
G	0046872	molecular_function	metal ion binding
G	0061621	biological_process	canonical glycolysis
G	0070062	cellular_component	extracellular exosome
G	0097060	cellular_component	synaptic membrane
H	0000015	cellular_component	phosphopyruvate hydratase complex
H	0000287	molecular_function	magnesium ion binding
H	0001917	cellular_component	photoreceptor inner segment
H	0004634	molecular_function	phosphopyruvate hydratase activity
H	0005515	molecular_function	protein binding
H	0005615	cellular_component	extracellular space
H	0005737	cellular_component	cytoplasm
H	0005829	cellular_component	cytosol
H	0005886	cellular_component	plasma membrane
H	0005938	cellular_component	cell cortex
H	0006094	biological_process	gluconeogenesis
H	0006096	biological_process	glycolytic process
H	0009410	biological_process	response to xenobiotic stimulus
H	0009986	cellular_component	cell surface
H	0016020	cellular_component	membrane
H	0016829	molecular_function	lyase activity
H	0019899	molecular_function	enzyme binding
H	0030426	cellular_component	growth cone
H	0032355	biological_process	response to estradiol
H	0042802	molecular_function	identical protein binding
H	0043025	cellular_component	neuronal cell body
H	0043204	cellular_component	perikaryon
H	0044877	molecular_function	protein-containing complex binding
H	0045121	cellular_component	membrane raft
H	0046872	molecular_function	metal ion binding
H	0061621	biological_process	canonical glycolysis
H	0070062	cellular_component	extracellular exosome
H	0097060	cellular_component	synaptic membrane

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue MG A 500

Chain	Residue
A	SER40
A	5TX502
A	HOH659
A	HOH676

---

site_id	AC2
Number of Residues	5
Details	binding site for residue MG A 501

Chain	Residue
A	ASP245
A	GLU293
A	ASP318
A	5TX502
A	HOH628

---

site_id	AC3
Number of Residues	19
Details	binding site for residue 5TX A 502

Chain	Residue
A	GLY38
A	ALA39
A	SER40
A	HIS158
A	GLN166
A	GLU167
A	ASP245
A	GLU293
A	ASP318
A	LEU341
A	LYS343
A	HIS371
A	ARG372
A	SER373
A	LYS394
A	MG500
A	MG501
A	HOH659
A	HOH676

---

site_id	AC4
Number of Residues	4
Details	binding site for residue MG B 500

Chain	Residue
B	SER40
B	5TX502
B	HOH659
B	HOH720

---

site_id	AC5
Number of Residues	6
Details	binding site for residue MG B 501

Chain	Residue
B	ASP245
B	GLU293
B	ASP318
B	LYS394
B	5TX502
B	HOH625

---

site_id	AC6
Number of Residues	19
Details	binding site for residue 5TX B 502

Chain	Residue
B	GLY38
B	ALA39
B	SER40
B	GLN166
B	GLU167
B	GLU210
B	ASP245
B	GLU293
B	ASP318
B	LEU341
B	LYS343
B	HIS371
B	ARG372
B	SER373
B	LYS394
B	MG500
B	MG501
B	HOH659
B	HOH720

---

site_id	AC7
Number of Residues	5
Details	binding site for residue MG C 500

Chain	Residue
C	ASP245
C	GLU293
C	ASP318
C	5TX503
C	HOH630

---

site_id	AC8
Number of Residues	4
Details	binding site for residue MG C 501

Chain	Residue
C	SER40
C	5TX503
C	HOH640
C	HOH744

---

site_id	AC9
Number of Residues	2
Details	binding site for residue PGE C 502

Chain	Residue
C	LYS5
C	TYR25

---

site_id	AD1
Number of Residues	16
Details	binding site for residue 5TX C 503

Chain	Residue
C	GLY38
C	ALA39
C	SER40
C	GLN166
C	GLU167
C	ASP245
C	GLU293
C	ASP318
C	LYS343
C	HIS371
C	ARG372
C	SER373
C	LYS394
C	MG500
C	MG501
C	HOH744

---

site_id	AD2
Number of Residues	5
Details	binding site for residue MG D 500

Chain	Residue
D	ASP245
D	GLU293
D	ASP318
D	5TX503
D	HOH613

---

site_id	AD3
Number of Residues	4
Details	binding site for residue MG D 501

Chain	Residue
D	SER40
D	5TX503
D	HOH687
D	HOH742

---

site_id	AD4
Number of Residues	3
Details	binding site for residue PGE D 502

Chain	Residue
D	LYS5
D	TRP7
D	TYR25

---

site_id	AD5
Number of Residues	18
Details	binding site for residue 5TX D 503

Chain	Residue
D	GLN166
D	GLU167
D	ASP245
D	GLU293
D	ASP318
D	LEU341
D	LYS343
D	HIS371
D	ARG372
D	SER373
D	LYS394
D	MG500
D	MG501
D	HOH687
D	HOH742
D	GLY38
D	ALA39
D	SER40

---

site_id	AD6
Number of Residues	5
Details	binding site for residue MG E 500

Chain	Residue
E	ASP245
E	GLU293
E	ASP318
E	5TX503
E	HOH643

---

site_id	AD7
Number of Residues	4
Details	binding site for residue MG E 501

Chain	Residue
E	SER40
E	5TX503
E	HOH683
E	HOH695

---

site_id	AD8
Number of Residues	2
Details	binding site for residue PGE E 502

Chain	Residue
E	LYS5
E	TYR25

---

site_id	AD9
Number of Residues	18
Details	binding site for residue 5TX E 503

Chain	Residue
E	GLY38
E	ALA39
E	SER40
E	GLN166
E	GLU167
E	ASP245
E	GLU293
E	ASP318
E	LEU341
E	LYS343
E	HIS371
E	ARG372
E	SER373
E	LYS394
E	MG500
E	MG501
E	HOH683
E	HOH695

---

site_id	AE1
Number of Residues	5
Details	binding site for residue MG F 500

Chain	Residue
F	ASP245
F	GLU293
F	ASP318
F	5TX503
F	HOH650

---

site_id	AE2
Number of Residues	4
Details	binding site for residue MG F 501

Chain	Residue
F	SER40
F	5TX503
F	HOH653
F	HOH655

---

site_id	AE3
Number of Residues	2
Details	binding site for residue PGE F 502

Chain	Residue
F	LYS5
F	TYR25

---

site_id	AE4
Number of Residues	19
Details	binding site for residue 5TX F 503

Chain	Residue
F	GLY38
F	ALA39
F	SER40
F	HIS158
F	GLN166
F	GLU167
F	ASP245
F	GLU293
F	ASP318
F	LEU341
F	LYS343
F	HIS371
F	ARG372
F	SER373
F	LYS394
F	MG500
F	MG501
F	HOH653
F	HOH655

---

site_id	AE5
Number of Residues	5
Details	binding site for residue MG G 500

Chain	Residue
G	ASP245
G	GLU293
G	ASP318
G	5TX503
G	HOH636

---

site_id	AE6
Number of Residues	4
Details	binding site for residue MG G 501

Chain	Residue
G	SER40
G	5TX503
G	HOH651
G	HOH667

---

site_id	AE7
Number of Residues	3
Details	binding site for residue PGE G 502

Chain	Residue
G	LYS5
G	TRP7
G	TYR25

---

site_id	AE8
Number of Residues	18
Details	binding site for residue 5TX G 503

Chain	Residue
G	GLY38
G	ALA39
G	SER40
G	GLN166
G	GLU167
G	ASP245
G	GLU293
G	ASP318
G	LEU341
G	LYS343
G	HIS371
G	ARG372
G	SER373
G	LYS394
G	MG500
G	MG501
G	HOH651
G	HOH667

---

site_id	AE9
Number of Residues	5
Details	binding site for residue MG H 500

Chain	Residue
H	ASP245
H	GLU293
H	ASP318
H	5TX503
H	HOH641

---

site_id	AF1
Number of Residues	4
Details	binding site for residue MG H 501

Chain	Residue
H	SER40
H	5TX503
H	HOH678
H	HOH749

---

site_id	AF2
Number of Residues	3
Details	binding site for residue PGE H 502

Chain	Residue
H	LYS5
H	TRP7
H	HOH690

---

site_id	AF3
Number of Residues	17
Details	binding site for residue 5TX H 503

Chain	Residue
H	GLY38
H	ALA39
H	SER40
H	GLN166
H	GLU167
H	ASP245
H	GLU293
H	ASP318
H	LYS343
H	HIS371
H	ARG372
H	SER373
H	LYS394
H	MG500
H	MG501
H	HOH678
H	HOH749

---

Functional Information from PROSITE/UniProt

site_id	PS00164
Number of Residues	14
Details	ENOLASE Enolase signature. LLLKvNQIGSVTEA

Chain	Residue	Details
A	LEU340-ALA353

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	8
Details	Active site: {"description":"Proton donor","evidences":[{"source":"UniProtKB","id":"P00924","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	8
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"UniProtKB","id":"P00924","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	8
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P06733","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	64
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P00924","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	8
Details	Binding site: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	Modified residue: {"description":"N-acetylserine","evidences":[{"source":"UniProtKB","id":"P06733","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	32
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P06733","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	8
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	8
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"PubMed","id":"15592455","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	24
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P17182","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	16
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P17183","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	8
Details	Modified residue: {"description":"N6-acetyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P17182","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	8
Details	Modified residue: {"description":"N6-acetyllysine; alternate","evidences":[{"source":"UniProtKB","id":"P06733","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI15
Number of Residues	8
Details	Modified residue: {"description":"Phosphotyrosine","evidences":[{"source":"UniProtKB","id":"P06733","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI16
Number of Residues	8
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P06733","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI17
Number of Residues	24
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P17182","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI18
Number of Residues	16
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate","evidences":[{"source":"UniProtKB","id":"P06733","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---