5DK4
Crystal structure analysis of Tryptophanyl-trna synthetase from Bacillus stearothermophilus in complex with indolmycin and Mg*ATP
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000166 | molecular_function | nucleotide binding |
| A | 0004812 | molecular_function | aminoacyl-tRNA ligase activity |
| A | 0004830 | molecular_function | tryptophan-tRNA ligase activity |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0006412 | biological_process | translation |
| A | 0006418 | biological_process | tRNA aminoacylation for protein translation |
| A | 0006436 | biological_process | tryptophanyl-tRNA aminoacylation |
| A | 0016874 | molecular_function | ligase activity |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 28 |
| Details | binding site for residue ATP A 401 |
| Chain | Residue |
| A | ILE8 |
| A | ASP146 |
| A | GLY180 |
| A | ALA181 |
| A | ARG182 |
| A | ILE183 |
| A | LYS192 |
| A | MSE193 |
| A | SER194 |
| A | LYS195 |
| A | SER196 |
| A | GLN9 |
| A | MG402 |
| A | 5BX404 |
| A | HOH520 |
| A | HOH525 |
| A | HOH537 |
| A | HOH548 |
| A | HOH589 |
| A | HOH591 |
| A | HOH619 |
| A | SER11 |
| A | GLY17 |
| A | ASN18 |
| A | GLY21 |
| A | LYS111 |
| A | VAL143 |
| A | GLY144 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | binding site for residue MG A 402 |
| Chain | Residue |
| A | GLN9 |
| A | ATP401 |
| A | 5BX404 |
| A | HOH525 |
| A | HOH589 |
| A | HOH591 |
| site_id | AC3 |
| Number of Residues | 5 |
| Details | binding site for residue GOL A 403 |
| Chain | Residue |
| A | LYS218 |
| A | THR222 |
| A | TYR265 |
| A | GLY266 |
| A | LYS269 |
| site_id | AC4 |
| Number of Residues | 14 |
| Details | binding site for residue 5BX A 404 |
| Chain | Residue |
| A | PHE5 |
| A | SER6 |
| A | GLY7 |
| A | GLN9 |
| A | HIS43 |
| A | TYR125 |
| A | MSE129 |
| A | ASP132 |
| A | VAL143 |
| A | GLN147 |
| A | ATP401 |
| A | MG402 |
| A | HOH525 |
| A | HOH589 |
Functional Information from PROSITE/UniProt
| site_id | PS00178 |
| Number of Residues | 10 |
| Details | AA_TRNA_LIGASE_I Aminoacyl-transfer RNA synthetases class-I signature. P..SGvITIGNY |
| Chain | Residue | Details |
| A | PRO10-TYR19 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 8 |
| Details | Motif: {"description":"'HIGH' region","evidences":[{"source":"HAMAP-Rule","id":"MF_00140","evidenceCode":"ECO:0000255"},{"evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 7 |
| Details | Binding site: {"evidences":[{"source":"HAMAP-Rule","id":"MF_00140","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"26555258","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"5DK4","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
Catalytic Information from CSA
| site_id | MCSA1 |
| Number of Residues | 3 |
| Details | M-CSA 481 |
| Chain | Residue | Details |
| A | LEU123 | electrostatic stabiliser |
| A | THR212 | electrostatic stabiliser |
| A | SER219 | electrostatic stabiliser |
