5DK4
Crystal structure analysis of Tryptophanyl-trna synthetase from Bacillus stearothermophilus in complex with indolmycin and Mg*ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-02-01 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.97949 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 62.041, 62.041, 219.058 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 31.020 - 1.900 |
R-factor | 0.1702 |
Rwork | 0.169 |
R-free | 0.18890 |
Structure solution method | SAD |
RMSD bond length | 0.005 |
RMSD bond angle | 0.994 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 31.020 |
High resolution limit [Å] | 1.900 |
Number of reflections | 34849 |
<I/σ(I)> | 10.7 |
Completeness [%] | 100.0 |
Redundancy | 15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | hepes, potassium citrate, magnesium chloride |