5CHV

Crystal structure of USP18-ISG15 complex

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004843	molecular_function	cysteine-type deubiquitinase activity
A	0016579	biological_process	protein deubiquitination
B	0004843	molecular_function	cysteine-type deubiquitinase activity
B	0016579	biological_process	protein deubiquitination
C	0005178	molecular_function	integrin binding
C	0005515	molecular_function	protein binding
C	0005576	cellular_component	extracellular region
C	0005737	cellular_component	cytoplasm
C	0007229	biological_process	integrin-mediated signaling pathway
C	0009615	biological_process	response to virus
C	0009617	biological_process	response to bacterium
C	0016567	biological_process	protein ubiquitination
C	0019941	biological_process	modification-dependent protein catabolic process
C	0030501	biological_process	positive regulation of bone mineralization
C	0031386	molecular_function	protein tag activity
C	0031397	biological_process	negative regulation of protein ubiquitination
C	0031625	molecular_function	ubiquitin protein ligase binding
C	0032020	biological_process	ISG15-protein conjugation
C	0032461	biological_process	positive regulation of protein oligomerization
C	0032649	biological_process	regulation of type II interferon production
C	0032728	biological_process	positive regulation of interferon-beta production
C	0032729	biological_process	positive regulation of type II interferon production
C	0032733	biological_process	positive regulation of interleukin-10 production
C	0034340	biological_process	response to type I interferon
C	0042742	biological_process	defense response to bacterium
C	0045071	biological_process	negative regulation of viral genome replication
C	0045087	biological_process	innate immune response
C	0045648	biological_process	positive regulation of erythrocyte differentiation
C	0051607	biological_process	defense response to virus
C	0060339	biological_process	negative regulation of type I interferon-mediated signaling pathway
C	0070585	biological_process	protein localization to mitochondrion
D	0005178	molecular_function	integrin binding
D	0005515	molecular_function	protein binding
D	0005576	cellular_component	extracellular region
D	0005737	cellular_component	cytoplasm
D	0007229	biological_process	integrin-mediated signaling pathway
D	0009615	biological_process	response to virus
D	0009617	biological_process	response to bacterium
D	0016567	biological_process	protein ubiquitination
D	0019941	biological_process	modification-dependent protein catabolic process
D	0030501	biological_process	positive regulation of bone mineralization
D	0031386	molecular_function	protein tag activity
D	0031397	biological_process	negative regulation of protein ubiquitination
D	0031625	molecular_function	ubiquitin protein ligase binding
D	0032020	biological_process	ISG15-protein conjugation
D	0032461	biological_process	positive regulation of protein oligomerization
D	0032649	biological_process	regulation of type II interferon production
D	0032728	biological_process	positive regulation of interferon-beta production
D	0032729	biological_process	positive regulation of type II interferon production
D	0032733	biological_process	positive regulation of interleukin-10 production
D	0034340	biological_process	response to type I interferon
D	0042742	biological_process	defense response to bacterium
D	0045071	biological_process	negative regulation of viral genome replication
D	0045087	biological_process	innate immune response
D	0045648	biological_process	positive regulation of erythrocyte differentiation
D	0051607	biological_process	defense response to virus
D	0060339	biological_process	negative regulation of type I interferon-mediated signaling pathway
D	0070585	biological_process	protein localization to mitochondrion

Functional Information from PDB Data

site_id	AC1
Number of Residues	4
Details	binding site for residue ZN A 401

Chain	Residue
A	CYS175
A	CYS178
A	CYS226
A	CYS229

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site_id	AC2
Number of Residues	5
Details	binding site for residue SO4 A 402

Chain	Residue
A	ASN128
A	HIS135
A	GLN139
A	LEU140
A	THR143

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site_id	AC3
Number of Residues	2
Details	binding site for residue CL A 403

Chain	Residue
A	SER268
A	HIS350

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site_id	AC4
Number of Residues	4
Details	binding site for residue ZN B 401

Chain	Residue
B	CYS175
B	CYS178
B	CYS226
B	CYS229

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site_id	AC5
Number of Residues	3
Details	binding site for residue SO4 B 402

Chain	Residue
B	ASN128
B	PRO130
B	GLN139

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site_id	AC6
Number of Residues	2
Details	binding site for residue CL B 403

Chain	Residue
B	MET72
B	ARG77

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site_id	AC7
Number of Residues	26
Details	binding site for Di-peptide CYS B 61 and AYE D 155

Chain	Residue
B	ASN56
B	GLN59
B	THR60
B	CYS62
B	LEU63
B	SER65
B	GLN134
B	THR153
B	ASP154
B	ASP156
B	LEU157
B	THR158
B	GLU159
B	GLY313
B	HIS314
B	TYR315
B	ASP332
D	LEU52
D	LEU56
D	THR57
D	LEU58
D	SER59
D	SER60
D	GLY62
D	LEU63
D	GLY154

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site_id	AC8
Number of Residues	8
Details	binding site for Di-peptide GLY D 154 and AYE D 155

Chain	Residue
B	CYS61
B	CYS62
B	GLN134
B	HIS135
B	GLY313
B	HIS314
B	TYR315
D	ARG153

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Functional Information from PROSITE/UniProt

site_id	PS00972
Number of Residues	16
Details	USP_1 Ubiquitin specific protease (USP) domain signature 1. GLhniGQtCCLNSlLQ

Chain	Residue	Details
A	GLY53-GLN68

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site_id	PS00973
Number of Residues	18
Details	USP_2 Ubiquitin specific protease (USP) domain signature 2. YeLfAViaHvGmadf..GHY

Chain	Residue	Details
A	TYR298-TYR315

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	SITE: Interacts with activating enzyme => ECO:0000250

Chain	Residue	Details
C	ARG151
D	ARG151

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site_id	SWS_FT_FI2
Number of Residues	4
Details	MOD_RES: S-nitrosocysteine => ECO:0000269|PubMed:18606809

Chain	Residue	Details
C	SER76
C	CYS144
D	SER76
D	CYS144

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site_id	SWS_FT_FI3
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor proteins) => ECO:0000255|PROSITE-ProRule:PRU00214

Chain	Residue	Details
C	AYE155
D	AYE155

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