5CHV

Crystal structure of USP18-ISG15 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-11-22
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	64.040, 72.806, 217.259
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.312 - 3.005
R-factor	0.2277
Rwork	0.225
R-free	0.28620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cht 5chf
RMSD bond length	0.008
RMSD bond angle	1.230
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.000		3.160
High resolution limit [Å]	3.000	10.000	3.000
Rmerge	0.627	0.158	2.934
Rmeas	0.653	0.166	3.054
Total number of observations	274828
Number of reflections	21117	643	3001
<I/σ(I)>	3.53	9.28	0.86
Completeness [%]	99.9	97.9	100
Redundancy	13

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	12.5% (w/v) PEG 1000, 12.5% (w/v) PEG 3350, 12.5% (v/v) MPD, 0.03 M NaNO3, 0.03 M Na2HPO4, 0.03 M (NH4)2SO4, 0.1 M MOPS/Hepes-NaOH, pH 7.5