5CC9
L28F E.coli dihydrofolate reductase complexed with 5,10-dideazatetrahydrofolate and oxidized nicotinamide adenine dinucleotide phosphate
Functional Information from GO Data
Chain | GOid | namespace | contents |
A | 0004146 | molecular_function | dihydrofolate reductase activity |
A | 0005515 | molecular_function | protein binding |
A | 0005542 | molecular_function | folic acid binding |
A | 0005829 | cellular_component | cytosol |
A | 0006730 | biological_process | one-carbon metabolic process |
A | 0009257 | biological_process | 10-formyltetrahydrofolate biosynthetic process |
A | 0009410 | biological_process | response to xenobiotic stimulus |
A | 0016491 | molecular_function | oxidoreductase activity |
A | 0031427 | biological_process | response to methotrexate |
A | 0046452 | biological_process | dihydrofolate metabolic process |
A | 0046654 | biological_process | tetrahydrofolate biosynthetic process |
A | 0046655 | biological_process | folic acid metabolic process |
A | 0046656 | biological_process | folic acid biosynthetic process |
A | 0046677 | biological_process | response to antibiotic |
A | 0050661 | molecular_function | NADP binding |
A | 0051870 | molecular_function | methotrexate binding |
A | 0051871 | molecular_function | dihydrofolic acid binding |
A | 0070401 | molecular_function | NADP+ binding |
A | 0070402 | molecular_function | NADPH binding |
Functional Information from PDB Data
site_id | AC1 |
Number of Residues | 31 |
Details | binding site for residue NAP A 201 |
Chain | Residue |
A | MET16 |
A | GLY96 |
A | GLY97 |
A | ARG98 |
A | VAL99 |
A | GLN102 |
A | ASP122 |
A | THR123 |
A | GLU134 |
A | SER135 |
A | VAL136 |
A | GLY43 |
A | HOH314 |
A | HOH320 |
A | HOH322 |
A | HOH325 |
A | HOH326 |
A | HOH337 |
A | HOH340 |
A | HOH347 |
A | HOH366 |
A | HOH409 |
A | ARG44 |
A | HOH428 |
A | HOH429 |
A | HIS45 |
A | THR46 |
A | LEU62 |
A | SER63 |
A | SER64 |
A | LYS76 |
site_id | AC2 |
Number of Residues | 20 |
Details | binding site for residue DDF A 202 |
Chain | Residue |
A | ILE5 |
A | ALA6 |
A | ALA7 |
A | ASP27 |
A | PHE28 |
A | PHE31 |
A | LYS32 |
A | ILE50 |
A | LEU54 |
A | ARG57 |
A | ILE94 |
A | TYR100 |
A | THR113 |
A | HOH316 |
A | HOH328 |
A | HOH343 |
A | HOH350 |
A | HOH375 |
A | HOH390 |
A | HOH408 |
Functional Information from PROSITE/UniProt
site_id | PS00075 |
Number of Residues | 23 |
Details | DHFR_1 Dihydrofolate reductase (DHFR) domain signature. VIGmenaMPWnlpa.DfawFkrnT |
Chain | Residue | Details |
A | VAL13-THR35 |
Functional Information from SwissProt/UniProt
site_id | SWS_FT_FI1 |
Number of Residues | 5 |
Details | BINDING: BINDING => ECO:0000305|PubMed:9012674 |
Chain | Residue | Details |
A | ILE5 | |
A | ASP27 | |
A | ARG52 | |
A | ARG57 | |
A | THR113 |
site_id | SWS_FT_FI2 |
Number of Residues | 6 |
Details | BINDING: BINDING => ECO:0000269|PubMed:19374017 |
Chain | Residue | Details |
A | ALA7 | |
A | VAL13 | |
A | HIS45 | |
A | SER63 | |
A | LYS76 | |
A | GLY95 |