Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4ZRU

X-ray crystal structure of Lymnaea stagnalis acetylcholine binding protein (Ls-AChBP) in complex with 3-[2-[(2S)-pyrrolidin-2-yl]ethynyl]pyridine (TI-5180)

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004888	molecular_function	transmembrane signaling receptor activity
A	0005216	molecular_function	monoatomic ion channel activity
A	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
A	0005576	cellular_component	extracellular region
A	0006811	biological_process	monoatomic ion transport
A	0007165	biological_process	signal transduction
A	0007271	biological_process	synaptic transmission, cholinergic
A	0015464	molecular_function	acetylcholine receptor activity
A	0016020	cellular_component	membrane
A	0022848	molecular_function	acetylcholine-gated monoatomic cation-selective channel activity
A	0034220	biological_process	monoatomic ion transmembrane transport
A	0035094	biological_process	response to nicotine
A	0042391	biological_process	regulation of membrane potential
A	0043005	cellular_component	neuron projection
A	0043083	cellular_component	synaptic cleft
A	0045202	cellular_component	synapse
A	0050877	biological_process	nervous system process
A	0060078	biological_process	regulation of postsynaptic membrane potential
A	0060079	biological_process	excitatory postsynaptic potential
A	0098794	cellular_component	postsynapse
B	0004888	molecular_function	transmembrane signaling receptor activity
B	0005216	molecular_function	monoatomic ion channel activity
B	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
B	0005576	cellular_component	extracellular region
B	0006811	biological_process	monoatomic ion transport
B	0007165	biological_process	signal transduction
B	0007271	biological_process	synaptic transmission, cholinergic
B	0015464	molecular_function	acetylcholine receptor activity
B	0016020	cellular_component	membrane
B	0022848	molecular_function	acetylcholine-gated monoatomic cation-selective channel activity
B	0034220	biological_process	monoatomic ion transmembrane transport
B	0035094	biological_process	response to nicotine
B	0042391	biological_process	regulation of membrane potential
B	0043005	cellular_component	neuron projection
B	0043083	cellular_component	synaptic cleft
B	0045202	cellular_component	synapse
B	0050877	biological_process	nervous system process
B	0060078	biological_process	regulation of postsynaptic membrane potential
B	0060079	biological_process	excitatory postsynaptic potential
B	0098794	cellular_component	postsynapse
C	0004888	molecular_function	transmembrane signaling receptor activity
C	0005216	molecular_function	monoatomic ion channel activity
C	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
C	0005576	cellular_component	extracellular region
C	0006811	biological_process	monoatomic ion transport
C	0007165	biological_process	signal transduction
C	0007271	biological_process	synaptic transmission, cholinergic
C	0015464	molecular_function	acetylcholine receptor activity
C	0016020	cellular_component	membrane
C	0022848	molecular_function	acetylcholine-gated monoatomic cation-selective channel activity
C	0034220	biological_process	monoatomic ion transmembrane transport
C	0035094	biological_process	response to nicotine
C	0042391	biological_process	regulation of membrane potential
C	0043005	cellular_component	neuron projection
C	0043083	cellular_component	synaptic cleft
C	0045202	cellular_component	synapse
C	0050877	biological_process	nervous system process
C	0060078	biological_process	regulation of postsynaptic membrane potential
C	0060079	biological_process	excitatory postsynaptic potential
C	0098794	cellular_component	postsynapse
D	0004888	molecular_function	transmembrane signaling receptor activity
D	0005216	molecular_function	monoatomic ion channel activity
D	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
D	0005576	cellular_component	extracellular region
D	0006811	biological_process	monoatomic ion transport
D	0007165	biological_process	signal transduction
D	0007271	biological_process	synaptic transmission, cholinergic
D	0015464	molecular_function	acetylcholine receptor activity
D	0016020	cellular_component	membrane
D	0022848	molecular_function	acetylcholine-gated monoatomic cation-selective channel activity
D	0034220	biological_process	monoatomic ion transmembrane transport
D	0035094	biological_process	response to nicotine
D	0042391	biological_process	regulation of membrane potential
D	0043005	cellular_component	neuron projection
D	0043083	cellular_component	synaptic cleft
D	0045202	cellular_component	synapse
D	0050877	biological_process	nervous system process
D	0060078	biological_process	regulation of postsynaptic membrane potential
D	0060079	biological_process	excitatory postsynaptic potential
D	0098794	cellular_component	postsynapse
E	0004888	molecular_function	transmembrane signaling receptor activity
E	0005216	molecular_function	monoatomic ion channel activity
E	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
E	0005576	cellular_component	extracellular region
E	0006811	biological_process	monoatomic ion transport
E	0007165	biological_process	signal transduction
E	0007271	biological_process	synaptic transmission, cholinergic
E	0015464	molecular_function	acetylcholine receptor activity
E	0016020	cellular_component	membrane
E	0022848	molecular_function	acetylcholine-gated monoatomic cation-selective channel activity
E	0034220	biological_process	monoatomic ion transmembrane transport
E	0035094	biological_process	response to nicotine
E	0042391	biological_process	regulation of membrane potential
E	0043005	cellular_component	neuron projection
E	0043083	cellular_component	synaptic cleft
E	0045202	cellular_component	synapse
E	0050877	biological_process	nervous system process
E	0060078	biological_process	regulation of postsynaptic membrane potential
E	0060079	biological_process	excitatory postsynaptic potential
E	0098794	cellular_component	postsynapse
F	0004888	molecular_function	transmembrane signaling receptor activity
F	0005216	molecular_function	monoatomic ion channel activity
F	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
F	0005576	cellular_component	extracellular region
F	0006811	biological_process	monoatomic ion transport
F	0007165	biological_process	signal transduction
F	0007271	biological_process	synaptic transmission, cholinergic
F	0015464	molecular_function	acetylcholine receptor activity
F	0016020	cellular_component	membrane
F	0022848	molecular_function	acetylcholine-gated monoatomic cation-selective channel activity
F	0034220	biological_process	monoatomic ion transmembrane transport
F	0035094	biological_process	response to nicotine
F	0042391	biological_process	regulation of membrane potential
F	0043005	cellular_component	neuron projection
F	0043083	cellular_component	synaptic cleft
F	0045202	cellular_component	synapse
F	0050877	biological_process	nervous system process
F	0060078	biological_process	regulation of postsynaptic membrane potential
F	0060079	biological_process	excitatory postsynaptic potential
F	0098794	cellular_component	postsynapse
G	0004888	molecular_function	transmembrane signaling receptor activity
G	0005216	molecular_function	monoatomic ion channel activity
G	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
G	0005576	cellular_component	extracellular region
G	0006811	biological_process	monoatomic ion transport
G	0007165	biological_process	signal transduction
G	0007271	biological_process	synaptic transmission, cholinergic
G	0015464	molecular_function	acetylcholine receptor activity
G	0016020	cellular_component	membrane
G	0022848	molecular_function	acetylcholine-gated monoatomic cation-selective channel activity
G	0034220	biological_process	monoatomic ion transmembrane transport
G	0035094	biological_process	response to nicotine
G	0042391	biological_process	regulation of membrane potential
G	0043005	cellular_component	neuron projection
G	0043083	cellular_component	synaptic cleft
G	0045202	cellular_component	synapse
G	0050877	biological_process	nervous system process
G	0060078	biological_process	regulation of postsynaptic membrane potential
G	0060079	biological_process	excitatory postsynaptic potential
G	0098794	cellular_component	postsynapse
H	0004888	molecular_function	transmembrane signaling receptor activity
H	0005216	molecular_function	monoatomic ion channel activity
H	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
H	0005576	cellular_component	extracellular region
H	0006811	biological_process	monoatomic ion transport
H	0007165	biological_process	signal transduction
H	0007271	biological_process	synaptic transmission, cholinergic
H	0015464	molecular_function	acetylcholine receptor activity
H	0016020	cellular_component	membrane
H	0022848	molecular_function	acetylcholine-gated monoatomic cation-selective channel activity
H	0034220	biological_process	monoatomic ion transmembrane transport
H	0035094	biological_process	response to nicotine
H	0042391	biological_process	regulation of membrane potential
H	0043005	cellular_component	neuron projection
H	0043083	cellular_component	synaptic cleft
H	0045202	cellular_component	synapse
H	0050877	biological_process	nervous system process
H	0060078	biological_process	regulation of postsynaptic membrane potential
H	0060079	biological_process	excitatory postsynaptic potential
H	0098794	cellular_component	postsynapse
I	0004888	molecular_function	transmembrane signaling receptor activity
I	0005216	molecular_function	monoatomic ion channel activity
I	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
I	0005576	cellular_component	extracellular region
I	0006811	biological_process	monoatomic ion transport
I	0007165	biological_process	signal transduction
I	0007271	biological_process	synaptic transmission, cholinergic
I	0015464	molecular_function	acetylcholine receptor activity
I	0016020	cellular_component	membrane
I	0022848	molecular_function	acetylcholine-gated monoatomic cation-selective channel activity
I	0034220	biological_process	monoatomic ion transmembrane transport
I	0035094	biological_process	response to nicotine
I	0042391	biological_process	regulation of membrane potential
I	0043005	cellular_component	neuron projection
I	0043083	cellular_component	synaptic cleft
I	0045202	cellular_component	synapse
I	0050877	biological_process	nervous system process
I	0060078	biological_process	regulation of postsynaptic membrane potential
I	0060079	biological_process	excitatory postsynaptic potential
I	0098794	cellular_component	postsynapse
J	0004888	molecular_function	transmembrane signaling receptor activity
J	0005216	molecular_function	monoatomic ion channel activity
J	0005230	molecular_function	extracellular ligand-gated monoatomic ion channel activity
J	0005576	cellular_component	extracellular region
J	0006811	biological_process	monoatomic ion transport
J	0007165	biological_process	signal transduction
J	0007271	biological_process	synaptic transmission, cholinergic
J	0015464	molecular_function	acetylcholine receptor activity
J	0016020	cellular_component	membrane
J	0022848	molecular_function	acetylcholine-gated monoatomic cation-selective channel activity
J	0034220	biological_process	monoatomic ion transmembrane transport
J	0035094	biological_process	response to nicotine
J	0042391	biological_process	regulation of membrane potential
J	0043005	cellular_component	neuron projection
J	0043083	cellular_component	synaptic cleft
J	0045202	cellular_component	synapse
J	0050877	biological_process	nervous system process
J	0060078	biological_process	regulation of postsynaptic membrane potential
J	0060079	biological_process	excitatory postsynaptic potential
J	0098794	cellular_component	postsynapse

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	binding site for residue PO4 A 301

Chain	Residue
A	ASP17
A	VAL18
A	ILE19
A	HIS145
B	ARG3
B	VAL74
B	SER75

site_id	AC2
Number of Residues	7
Details	binding site for residue PO4 A 302

Chain	Residue
A	SER75
E	ASP17
E	VAL18
E	ILE19
E	HIS145
A	ARG3
A	VAL74

site_id	AC3
Number of Residues	6
Details	binding site for residue TI9 A 303

Chain	Residue
A	TYR89
A	TRP143
A	TYR192
B	LEU112
B	MET114
B	HOH459

site_id	AC4
Number of Residues	8
Details	binding site for residue PO4 B 301

Chain	Residue
B	ASP17
B	VAL18
B	ILE19
B	HIS145
B	HOH498
C	ARG3
C	VAL74
C	SER75

site_id	AC5
Number of Residues	5
Details	binding site for residue TI9 B 302

Chain	Residue
B	TYR89
B	TRP143
B	TYR192
C	LEU112
C	HOH464

site_id	AC6
Number of Residues	7
Details	binding site for residue PO4 C 301

Chain	Residue
C	ASP17
C	VAL18
C	ILE19
C	HIS145
D	ARG3
D	VAL74
D	SER75

site_id	AC7
Number of Residues	6
Details	binding site for residue TI9 C 302

Chain	Residue
C	TYR89
C	TRP143
C	TYR192
D	LEU112
D	MET114
D	HOH468

site_id	AC8
Number of Residues	8
Details	binding site for residue PO4 D 301

Chain	Residue
D	ASP17
D	VAL18
D	ILE19
D	HIS145
D	HOH475
E	ARG3
E	VAL74
E	SER75

site_id	AC9
Number of Residues	5
Details	binding site for residue TI9 D 302

Chain	Residue
D	TYR89
D	TRP143
D	TYR192
D	HOH458
E	LEU112

site_id	AD1
Number of Residues	7
Details	binding site for residue TI9 E 301

Chain	Residue
A	TRP53
A	LEU112
A	MET114
A	HOH1863
E	TYR89
E	TRP143
E	TYR192

site_id	AD2
Number of Residues	7
Details	binding site for residue PO4 F 301

Chain	Residue
F	ARG3
F	VAL74
F	SER75
J	ASP17
J	VAL18
J	ILE19
J	HIS145

site_id	AD3
Number of Residues	8
Details	binding site for residue PO4 F 302

Chain	Residue
F	ASP17
F	VAL18
F	ILE19
F	HIS145
F	HOH472
G	ARG3
G	VAL74
G	SER75

site_id	AD4
Number of Residues	7
Details	binding site for residue TI9 F 303

Chain	Residue
F	TYR89
F	TRP143
F	TYR192
G	ARG104
G	LEU112
G	MET114
G	HOH452

site_id	AD5
Number of Residues	7
Details	binding site for residue PO4 G 301

Chain	Residue
G	ASP17
G	VAL18
G	ILE19
G	HIS145
H	ARG3
H	VAL74
H	SER75

site_id	AD6
Number of Residues	6
Details	binding site for residue TI9 G 302

Chain	Residue
H	LEU112
H	HOH452
G	TYR89
G	TRP143
G	TYR192
H	ARG104

site_id	AD7
Number of Residues	5
Details	binding site for residue TI9 H 301

Chain	Residue
H	TYR89
H	TRP143
H	TYR192
H	HOH453
I	LEU112

site_id	AD8
Number of Residues	8
Details	binding site for residue PO4 I 301

Chain	Residue
I	ASP17
I	VAL18
I	ILE19
I	HIS145
I	HOH495
J	ARG3
J	VAL74
J	SER75

site_id	AD9
Number of Residues	8
Details	binding site for residue PO4 I 302

Chain	Residue
H	ASP17
H	VAL18
H	ILE19
H	HIS145
H	HOH438
I	ARG3
I	VAL74
I	SER75

site_id	AE1
Number of Residues	7
Details	binding site for residue TI9 I 303

Chain	Residue
I	TYR89
I	TRP143
I	TYR192
I	HOH463
J	ARG104
J	LEU112
J	MET114

site_id	AE2
Number of Residues	6
Details	binding site for residue TI9 J 301

Chain	Residue
F	LEU112
F	MET114
F	HOH478
J	TYR89
J	TRP143
J	TYR192

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	10
Details	CARBOHYD: N-linked (GlcNAc...) asparagine => ECO:0000305

Chain	Residue	Details
A	ASN66
B	ASN66
C	ASN66
D	ASN66
E	ASN66
F	ASN66
G	ASN66
H	ASN66
I	ASN66
J	ASN66

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)