4XYK
Crystal structure of human phosphofructokinase-1 in complex with ADP, Northeast Structural Genomics Consortium Target HR9275
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003872 | molecular_function | 6-phosphofructokinase activity |
| A | 0005515 | molecular_function | protein binding |
| A | 0005524 | molecular_function | ATP binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005829 | cellular_component | cytosol |
| A | 0005945 | cellular_component | 6-phosphofructokinase complex |
| A | 0006002 | biological_process | fructose 6-phosphate metabolic process |
| A | 0006096 | biological_process | glycolytic process |
| A | 0016020 | cellular_component | membrane |
| A | 0016208 | molecular_function | AMP binding |
| A | 0016301 | molecular_function | kinase activity |
| A | 0030388 | biological_process | fructose 1,6-bisphosphate metabolic process |
| A | 0042802 | molecular_function | identical protein binding |
| A | 0044877 | molecular_function | protein-containing complex binding |
| A | 0045296 | molecular_function | cadherin binding |
| A | 0046872 | molecular_function | metal ion binding |
| A | 0048029 | molecular_function | monosaccharide binding |
| A | 0061621 | biological_process | canonical glycolysis |
| A | 0070062 | cellular_component | extracellular exosome |
| A | 0070095 | molecular_function | fructose-6-phosphate binding |
| A | 1990830 | biological_process | cellular response to leukemia inhibitory factor |
| B | 0003872 | molecular_function | 6-phosphofructokinase activity |
| B | 0005515 | molecular_function | protein binding |
| B | 0005524 | molecular_function | ATP binding |
| B | 0005634 | cellular_component | nucleus |
| B | 0005737 | cellular_component | cytoplasm |
| B | 0005829 | cellular_component | cytosol |
| B | 0005945 | cellular_component | 6-phosphofructokinase complex |
| B | 0006002 | biological_process | fructose 6-phosphate metabolic process |
| B | 0006096 | biological_process | glycolytic process |
| B | 0016020 | cellular_component | membrane |
| B | 0016208 | molecular_function | AMP binding |
| B | 0016301 | molecular_function | kinase activity |
| B | 0030388 | biological_process | fructose 1,6-bisphosphate metabolic process |
| B | 0042802 | molecular_function | identical protein binding |
| B | 0044877 | molecular_function | protein-containing complex binding |
| B | 0045296 | molecular_function | cadherin binding |
| B | 0046872 | molecular_function | metal ion binding |
| B | 0048029 | molecular_function | monosaccharide binding |
| B | 0061621 | biological_process | canonical glycolysis |
| B | 0070062 | cellular_component | extracellular exosome |
| B | 0070095 | molecular_function | fructose-6-phosphate binding |
| B | 1990830 | biological_process | cellular response to leukemia inhibitory factor |
| C | 0003872 | molecular_function | 6-phosphofructokinase activity |
| C | 0005515 | molecular_function | protein binding |
| C | 0005524 | molecular_function | ATP binding |
| C | 0005634 | cellular_component | nucleus |
| C | 0005737 | cellular_component | cytoplasm |
| C | 0005829 | cellular_component | cytosol |
| C | 0005945 | cellular_component | 6-phosphofructokinase complex |
| C | 0006002 | biological_process | fructose 6-phosphate metabolic process |
| C | 0006096 | biological_process | glycolytic process |
| C | 0016020 | cellular_component | membrane |
| C | 0016208 | molecular_function | AMP binding |
| C | 0016301 | molecular_function | kinase activity |
| C | 0030388 | biological_process | fructose 1,6-bisphosphate metabolic process |
| C | 0042802 | molecular_function | identical protein binding |
| C | 0044877 | molecular_function | protein-containing complex binding |
| C | 0045296 | molecular_function | cadherin binding |
| C | 0046872 | molecular_function | metal ion binding |
| C | 0048029 | molecular_function | monosaccharide binding |
| C | 0061621 | biological_process | canonical glycolysis |
| C | 0070062 | cellular_component | extracellular exosome |
| C | 0070095 | molecular_function | fructose-6-phosphate binding |
| C | 1990830 | biological_process | cellular response to leukemia inhibitory factor |
| D | 0003872 | molecular_function | 6-phosphofructokinase activity |
| D | 0005515 | molecular_function | protein binding |
| D | 0005524 | molecular_function | ATP binding |
| D | 0005634 | cellular_component | nucleus |
| D | 0005737 | cellular_component | cytoplasm |
| D | 0005829 | cellular_component | cytosol |
| D | 0005945 | cellular_component | 6-phosphofructokinase complex |
| D | 0006002 | biological_process | fructose 6-phosphate metabolic process |
| D | 0006096 | biological_process | glycolytic process |
| D | 0016020 | cellular_component | membrane |
| D | 0016208 | molecular_function | AMP binding |
| D | 0016301 | molecular_function | kinase activity |
| D | 0030388 | biological_process | fructose 1,6-bisphosphate metabolic process |
| D | 0042802 | molecular_function | identical protein binding |
| D | 0044877 | molecular_function | protein-containing complex binding |
| D | 0045296 | molecular_function | cadherin binding |
| D | 0046872 | molecular_function | metal ion binding |
| D | 0048029 | molecular_function | monosaccharide binding |
| D | 0061621 | biological_process | canonical glycolysis |
| D | 0070062 | cellular_component | extracellular exosome |
| D | 0070095 | molecular_function | fructose-6-phosphate binding |
| D | 1990830 | biological_process | cellular response to leukemia inhibitory factor |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 11 |
| Details | binding site for residue ADP A 801 |
| Chain | Residue |
| A | SER32 |
| A | SER130 |
| D | GLN99 |
| A | GLY33 |
| A | TYR64 |
| A | ARG97 |
| A | CYS98 |
| A | ARG102 |
| A | GLY127 |
| A | ASP128 |
| A | GLY129 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | binding site for residue PO4 A 802 |
| Chain | Residue |
| A | GLY233 |
| A | LYS264 |
| A | ARG430 |
| A | ARG434 |
| A | GLY467 |
| A | GLY468 |
| site_id | AC3 |
| Number of Residues | 6 |
| Details | binding site for residue PO4 A 803 |
| Chain | Residue |
| A | ARG44 |
| A | ARG48 |
| A | VAL82 |
| A | SER83 |
| A | SER84 |
| A | ARG632 |
| site_id | AC4 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 A 804 |
| Chain | Residue |
| A | ARG481 |
| A | HIS671 |
| B | ARG576 |
| B | ARG665 |
| site_id | AC5 |
| Number of Residues | 13 |
| Details | binding site for residue ADP B 801 |
| Chain | Residue |
| B | GLY33 |
| B | GLY34 |
| B | TYR64 |
| B | ARG97 |
| B | CYS98 |
| B | ARG102 |
| B | GLY127 |
| B | ASP128 |
| B | GLY129 |
| B | SER130 |
| B | GLY133 |
| B | SER173 |
| B | ARG219 |
| site_id | AC6 |
| Number of Residues | 4 |
| Details | binding site for residue PO4 B 802 |
| Chain | Residue |
| B | GLY233 |
| B | LYS264 |
| B | ARG430 |
| B | ARG434 |
| site_id | AC7 |
| Number of Residues | 6 |
| Details | binding site for residue PO4 B 803 |
| Chain | Residue |
| B | ARG44 |
| B | ARG48 |
| B | SER83 |
| B | SER84 |
| B | GLY599 |
| B | ARG632 |
| site_id | AC8 |
| Number of Residues | 12 |
| Details | binding site for residue ADP C 801 |
| Chain | Residue |
| C | GLY33 |
| C | GLY34 |
| C | ARG97 |
| C | CYS98 |
| C | PHE101 |
| C | ARG102 |
| C | GLY126 |
| C | GLY127 |
| C | ASP128 |
| C | GLY129 |
| C | SER130 |
| C | LEU136 |
| site_id | AC9 |
| Number of Residues | 6 |
| Details | binding site for residue PO4 C 802 |
| Chain | Residue |
| C | GLY233 |
| C | LYS264 |
| C | ARG430 |
| C | ARG434 |
| C | GLY467 |
| C | GLY468 |
| site_id | AD1 |
| Number of Residues | 5 |
| Details | binding site for residue PO4 C 803 |
| Chain | Residue |
| C | ARG44 |
| C | ARG48 |
| C | SER83 |
| C | SER84 |
| C | ARG632 |
| site_id | AD2 |
| Number of Residues | 3 |
| Details | binding site for residue PO4 C 804 |
| Chain | Residue |
| C | HIS671 |
| C | GLN674 |
| D | ARG665 |
| site_id | AD3 |
| Number of Residues | 5 |
| Details | binding site for residue PO4 C 805 |
| Chain | Residue |
| C | ARG97 |
| C | ARG219 |
| C | ARG310 |
| D | ARG210 |
| D | ARG301 |
| site_id | AD4 |
| Number of Residues | 3 |
| Details | binding site for residue PO4 D 801 |
| Chain | Residue |
| C | ARG576 |
| C | ARG665 |
| D | ARG481 |
| site_id | AD5 |
| Number of Residues | 13 |
| Details | binding site for residue ADP D 802 |
| Chain | Residue |
| A | GLN99 |
| D | SER32 |
| D | GLY33 |
| D | TYR64 |
| D | ARG97 |
| D | CYS98 |
| D | ARG102 |
| D | GLY127 |
| D | ASP128 |
| D | GLY129 |
| D | SER130 |
| D | GLY133 |
| D | LEU136 |
| site_id | AD6 |
| Number of Residues | 6 |
| Details | binding site for residue PO4 D 803 |
| Chain | Residue |
| D | ARG430 |
| D | ARG434 |
| D | GLY467 |
| D | GLY468 |
| D | GLY233 |
| D | LYS264 |
| site_id | AD7 |
| Number of Residues | 6 |
| Details | binding site for residue PO4 D 804 |
| Chain | Residue |
| D | ARG44 |
| D | ARG48 |
| D | SER83 |
| D | SER84 |
| D | LYS567 |
| D | ARG632 |
Functional Information from PROSITE/UniProt
| site_id | PS00433 |
| Number of Residues | 19 |
| Details | PHOSPHOFRUCTOKINASE Phosphofructokinase signature. RvtilGHvQRGGtpsafDR |
| Chain | Residue | Details |
| A | ARG301-ARG319 | |
| A | ARG665-ARG683 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 4 |
| Details | ACT_SITE: Proton acceptor => ECO:0000255|HAMAP-Rule:MF_03184 |
| Chain | Residue | Details |
| A | ASP175 | |
| B | ASP175 | |
| C | ASP175 | |
| D | ASP175 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 32 |
| Details | BINDING: BINDING => ECO:0000255|HAMAP-Rule:MF_03184 |
| Chain | Residue | Details |
| A | GLY34 | |
| B | ARG97 | |
| B | GLY127 | |
| B | ASP128 | |
| B | ARG210 | |
| B | ARG301 | |
| B | ARG576 | |
| B | ARG665 | |
| C | GLY34 | |
| C | ARG97 | |
| C | GLY127 | |
| A | ARG97 | |
| C | ASP128 | |
| C | ARG210 | |
| C | ARG301 | |
| C | ARG576 | |
| C | ARG665 | |
| D | GLY34 | |
| D | ARG97 | |
| D | GLY127 | |
| D | ASP128 | |
| D | ARG210 | |
| A | GLY127 | |
| D | ARG301 | |
| D | ARG576 | |
| D | ARG665 | |
| A | ASP128 | |
| A | ARG210 | |
| A | ARG301 | |
| A | ARG576 | |
| A | ARG665 | |
| B | GLY34 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 40 |
| Details | BINDING: in other chain => ECO:0000255|HAMAP-Rule:MF_03184 |
| Chain | Residue | Details |
| A | SER173 | |
| A | ARG744 | |
| B | SER173 | |
| B | MET217 | |
| B | GLU273 | |
| B | HIS307 | |
| B | ARG481 | |
| B | THR538 | |
| B | MET583 | |
| B | GLU639 | |
| B | HIS671 | |
| A | MET217 | |
| B | ARG744 | |
| C | SER173 | |
| C | MET217 | |
| C | GLU273 | |
| C | HIS307 | |
| C | ARG481 | |
| C | THR538 | |
| C | MET583 | |
| C | GLU639 | |
| C | HIS671 | |
| A | GLU273 | |
| C | ARG744 | |
| D | SER173 | |
| D | MET217 | |
| D | GLU273 | |
| D | HIS307 | |
| D | ARG481 | |
| D | THR538 | |
| D | MET583 | |
| D | GLU639 | |
| D | HIS671 | |
| A | HIS307 | |
| D | ARG744 | |
| A | ARG481 | |
| A | THR538 | |
| A | MET583 | |
| A | GLU639 | |
| A | HIS671 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 4 |
| Details | MOD_RES: N-acetylmethionine => ECO:0007744|PubMed:19413330 |
| Chain | Residue | Details |
| A | MET1 | |
| B | MET1 | |
| C | MET1 | |
| D | MET1 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 8 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | SER6 | |
| A | SER21 | |
| B | SER6 | |
| B | SER21 | |
| C | SER6 | |
| C | SER21 | |
| D | SER6 | |
| D | SER21 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P47859 |
| Chain | Residue | Details |
| A | SER12 | |
| B | SER12 | |
| C | SER12 | |
| D | SER12 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P47860 |
| Chain | Residue | Details |
| A | SER142 | |
| B | SER142 | |
| C | SER142 | |
| D | SER142 |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0000269|PubMed:33607258, ECO:0007744|PubMed:16964243, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163 |
| Chain | Residue | Details |
| A | SER386 | |
| B | SER386 | |
| C | SER386 | |
| D | SER386 |
| site_id | SWS_FT_FI9 |
| Number of Residues | 12 |
| Details | MOD_RES: N6-acetyllysine => ECO:0007744|PubMed:19608861 |
| Chain | Residue | Details |
| A | LYS395 | |
| D | LYS395 | |
| D | LYS486 | |
| D | LYS688 | |
| A | LYS486 | |
| A | LYS688 | |
| B | LYS395 | |
| B | LYS486 | |
| B | LYS688 | |
| C | LYS395 | |
| C | LYS486 | |
| C | LYS688 |
| site_id | SWS_FT_FI10 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphotyrosine => ECO:0007744|PubMed:15592455 |
| Chain | Residue | Details |
| A | TYR651 | |
| B | TYR651 | |
| C | TYR651 | |
| D | TYR651 |
| site_id | SWS_FT_FI11 |
| Number of Residues | 4 |
| Details | MOD_RES: Phosphoserine => ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:18691976, ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231 |
| Chain | Residue | Details |
| A | SER783 | |
| B | SER783 | |
| C | SER783 | |
| D | SER783 |
| site_id | SWS_FT_FI12 |
| Number of Residues | 4 |
| Details | CARBOHYD: O-linked (GlcNAc) serine => ECO:0000250 |
| Chain | Residue | Details |
| A | SER540 | |
| B | SER540 | |
| C | SER540 | |
| D | SER540 |
