Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4WKG

The crystal structure of apo ArnA features an unexpected central binding pocket and provides an explanation for enzymatic coop-erativity

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003824	molecular_function	catalytic activity
A	0005515	molecular_function	protein binding
A	0009058	biological_process	biosynthetic process
A	0009103	biological_process	lipopolysaccharide biosynthetic process
A	0009245	biological_process	lipid A biosynthetic process
A	0016491	molecular_function	oxidoreductase activity
A	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
A	0016740	molecular_function	transferase activity
A	0016742	molecular_function	hydroxymethyl-, formyl- and related transferase activity
A	0016831	molecular_function	carboxy-lyase activity
A	0032991	cellular_component	protein-containing complex
A	0033320	biological_process	UDP-D-xylose biosynthetic process
A	0042802	molecular_function	identical protein binding
A	0046493	biological_process	lipid A metabolic process
A	0046677	biological_process	response to antibiotic
A	0048040	molecular_function	UDP-glucuronate decarboxylase activity
A	0070403	molecular_function	NAD+ binding
A	0099618	molecular_function	UDP-glucuronic acid dehydrogenase activity
A	0099619	molecular_function	UDP-4-amino-4-deoxy-L-arabinose formyltransferase activity
A	2001315	biological_process	UDP-4-deoxy-4-formamido-beta-L-arabinopyranose biosynthetic process
B	0003824	molecular_function	catalytic activity
B	0005515	molecular_function	protein binding
B	0009058	biological_process	biosynthetic process
B	0009103	biological_process	lipopolysaccharide biosynthetic process
B	0009245	biological_process	lipid A biosynthetic process
B	0016491	molecular_function	oxidoreductase activity
B	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
B	0016740	molecular_function	transferase activity
B	0016742	molecular_function	hydroxymethyl-, formyl- and related transferase activity
B	0016831	molecular_function	carboxy-lyase activity
B	0032991	cellular_component	protein-containing complex
B	0033320	biological_process	UDP-D-xylose biosynthetic process
B	0042802	molecular_function	identical protein binding
B	0046493	biological_process	lipid A metabolic process
B	0046677	biological_process	response to antibiotic
B	0048040	molecular_function	UDP-glucuronate decarboxylase activity
B	0070403	molecular_function	NAD+ binding
B	0099618	molecular_function	UDP-glucuronic acid dehydrogenase activity
B	0099619	molecular_function	UDP-4-amino-4-deoxy-L-arabinose formyltransferase activity
B	2001315	biological_process	UDP-4-deoxy-4-formamido-beta-L-arabinopyranose biosynthetic process
C	0003824	molecular_function	catalytic activity
C	0005515	molecular_function	protein binding
C	0009058	biological_process	biosynthetic process
C	0009103	biological_process	lipopolysaccharide biosynthetic process
C	0009245	biological_process	lipid A biosynthetic process
C	0016491	molecular_function	oxidoreductase activity
C	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
C	0016740	molecular_function	transferase activity
C	0016742	molecular_function	hydroxymethyl-, formyl- and related transferase activity
C	0016831	molecular_function	carboxy-lyase activity
C	0032991	cellular_component	protein-containing complex
C	0033320	biological_process	UDP-D-xylose biosynthetic process
C	0042802	molecular_function	identical protein binding
C	0046493	biological_process	lipid A metabolic process
C	0046677	biological_process	response to antibiotic
C	0048040	molecular_function	UDP-glucuronate decarboxylase activity
C	0070403	molecular_function	NAD+ binding
C	0099618	molecular_function	UDP-glucuronic acid dehydrogenase activity
C	0099619	molecular_function	UDP-4-amino-4-deoxy-L-arabinose formyltransferase activity
C	2001315	biological_process	UDP-4-deoxy-4-formamido-beta-L-arabinopyranose biosynthetic process
D	0003824	molecular_function	catalytic activity
D	0005515	molecular_function	protein binding
D	0009058	biological_process	biosynthetic process
D	0009103	biological_process	lipopolysaccharide biosynthetic process
D	0009245	biological_process	lipid A biosynthetic process
D	0016491	molecular_function	oxidoreductase activity
D	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
D	0016740	molecular_function	transferase activity
D	0016742	molecular_function	hydroxymethyl-, formyl- and related transferase activity
D	0016831	molecular_function	carboxy-lyase activity
D	0032991	cellular_component	protein-containing complex
D	0033320	biological_process	UDP-D-xylose biosynthetic process
D	0042802	molecular_function	identical protein binding
D	0046493	biological_process	lipid A metabolic process
D	0046677	biological_process	response to antibiotic
D	0048040	molecular_function	UDP-glucuronate decarboxylase activity
D	0070403	molecular_function	NAD+ binding
D	0099618	molecular_function	UDP-glucuronic acid dehydrogenase activity
D	0099619	molecular_function	UDP-4-amino-4-deoxy-L-arabinose formyltransferase activity
D	2001315	biological_process	UDP-4-deoxy-4-formamido-beta-L-arabinopyranose biosynthetic process
E	0003824	molecular_function	catalytic activity
E	0005515	molecular_function	protein binding
E	0009058	biological_process	biosynthetic process
E	0009103	biological_process	lipopolysaccharide biosynthetic process
E	0009245	biological_process	lipid A biosynthetic process
E	0016491	molecular_function	oxidoreductase activity
E	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
E	0016740	molecular_function	transferase activity
E	0016742	molecular_function	hydroxymethyl-, formyl- and related transferase activity
E	0016831	molecular_function	carboxy-lyase activity
E	0032991	cellular_component	protein-containing complex
E	0033320	biological_process	UDP-D-xylose biosynthetic process
E	0042802	molecular_function	identical protein binding
E	0046493	biological_process	lipid A metabolic process
E	0046677	biological_process	response to antibiotic
E	0048040	molecular_function	UDP-glucuronate decarboxylase activity
E	0070403	molecular_function	NAD+ binding
E	0099618	molecular_function	UDP-glucuronic acid dehydrogenase activity
E	0099619	molecular_function	UDP-4-amino-4-deoxy-L-arabinose formyltransferase activity
E	2001315	biological_process	UDP-4-deoxy-4-formamido-beta-L-arabinopyranose biosynthetic process
F	0003824	molecular_function	catalytic activity
F	0005515	molecular_function	protein binding
F	0009058	biological_process	biosynthetic process
F	0009103	biological_process	lipopolysaccharide biosynthetic process
F	0009245	biological_process	lipid A biosynthetic process
F	0016491	molecular_function	oxidoreductase activity
F	0016616	molecular_function	oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
F	0016740	molecular_function	transferase activity
F	0016742	molecular_function	hydroxymethyl-, formyl- and related transferase activity
F	0016831	molecular_function	carboxy-lyase activity
F	0032991	cellular_component	protein-containing complex
F	0033320	biological_process	UDP-D-xylose biosynthetic process
F	0042802	molecular_function	identical protein binding
F	0046493	biological_process	lipid A metabolic process
F	0046677	biological_process	response to antibiotic
F	0048040	molecular_function	UDP-glucuronate decarboxylase activity
F	0070403	molecular_function	NAD+ binding
F	0099618	molecular_function	UDP-glucuronic acid dehydrogenase activity
F	0099619	molecular_function	UDP-4-amino-4-deoxy-L-arabinose formyltransferase activity
F	2001315	biological_process	UDP-4-deoxy-4-formamido-beta-L-arabinopyranose biosynthetic process

Functional Information from PDB Data

site_id	AC1
Number of Residues	5
Details	binding site for residue DTT A 701

Chain	Residue
A	HIS372
A	TRP375
B	HIS372
B	TRP375
C	TRP375

site_id	AC2
Number of Residues	3
Details	binding site for residue ACT A 702

Chain	Residue
A	TYR83
A	LEU117
A	HIS162

site_id	AC3
Number of Residues	3
Details	binding site for residue DTT D 701

Chain	Residue
E	TRP375
F	TRP375
D	TRP375

site_id	AC4
Number of Residues	3
Details	binding site for residue ACT F 701

Chain	Residue
F	TYR83
F	LEU117
F	HIS162

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	6
Details	ACT_SITE: Proton donor; for formyltransferase activity

Chain	Residue	Details
A	HIS104
B	HIS104
C	HIS104
D	HIS104
E	HIS104
F	HIS104

site_id	SWS_FT_FI2
Number of Residues	6
Details	ACT_SITE: Proton acceptor; for decarboxylase activity

Chain	Residue	Details
A	GLU434
B	GLU434
C	GLU434
D	GLU434
E	GLU434
F	GLU434

site_id	SWS_FT_FI3
Number of Residues	6
Details	ACT_SITE: Proton donor; for decarboxylase activity

Chain	Residue	Details
A	ARG619
B	ARG619
C	ARG619
D	ARG619
E	ARG619
F	ARG619

site_id	SWS_FT_FI4
Number of Residues	72
Details	BINDING:

Chain	Residue	Details
A	HIS86
A	ASN492
A	LYS526
A	TYR613
B	HIS86
B	ARG114
B	VAL136
B	ASP347
B	ASP368
B	ALA393
B	TYR398
A	ARG114
B	THR432
B	ARG460
B	ASN492
B	LYS526
B	TYR613
C	HIS86
C	ARG114
C	VAL136
C	ASP347
C	ASP368
A	VAL136
C	ALA393
C	TYR398
C	THR432
C	ARG460
C	ASN492
C	LYS526
C	TYR613
D	HIS86
D	ARG114
D	VAL136
A	ASP347
D	ASP347
D	ASP368
D	ALA393
D	TYR398
D	THR432
D	ARG460
D	ASN492
D	LYS526
D	TYR613
E	HIS86
A	ASP368
E	ARG114
E	VAL136
E	ASP347
E	ASP368
E	ALA393
E	TYR398
E	THR432
E	ARG460
E	ASN492
E	LYS526
A	ALA393
E	TYR613
F	HIS86
F	ARG114
F	VAL136
F	ASP347
F	ASP368
F	ALA393
F	TYR398
F	THR432
F	ARG460
A	TYR398
F	ASN492
F	LYS526
F	TYR613
A	THR432
A	ARG460

site_id	SWS_FT_FI5
Number of Residues	6
Details	SITE: Transition state stabilizer

Chain	Residue	Details
A	ASN102
B	ASN102
C	ASN102
D	ASN102
E	ASN102
F	ASN102

site_id	SWS_FT_FI6
Number of Residues	6
Details	SITE: Raises pKa of active site His

Chain	Residue	Details
A	ASP140
B	ASP140
C	ASP140
D	ASP140
E	ASP140
F	ASP140

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)