Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4V24

Sphingosine kinase 1 in complex with PF-543

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000287	molecular_function	magnesium ion binding
A	0001727	molecular_function	lipid kinase activity
A	0003376	biological_process	sphingosine-1-phosphate receptor signaling pathway
A	0003677	molecular_function	DNA binding
A	0005515	molecular_function	protein binding
A	0005516	molecular_function	calmodulin binding
A	0005524	molecular_function	ATP binding
A	0005634	cellular_component	nucleus
A	0005654	cellular_component	nucleoplasm
A	0005737	cellular_component	cytoplasm
A	0005829	cellular_component	cytosol
A	0005886	cellular_component	plasma membrane
A	0005905	cellular_component	clathrin-coated pit
A	0006473	biological_process	protein acetylation
A	0006629	biological_process	lipid metabolic process
A	0006670	biological_process	sphingosine metabolic process
A	0008289	molecular_function	lipid binding
A	0008481	molecular_function	sphingosine kinase activity
A	0010008	cellular_component	endosome membrane
A	0010468	biological_process	regulation of gene expression
A	0010803	biological_process	regulation of tumor necrosis factor-mediated signaling pathway
A	0016301	molecular_function	kinase activity
A	0016407	molecular_function	acetyltransferase activity
A	0016740	molecular_function	transferase activity
A	0019722	biological_process	calcium-mediated signaling
A	0030100	biological_process	regulation of endocytosis
A	0030139	cellular_component	endocytic vesicle
A	0030148	biological_process	sphingolipid biosynthetic process
A	0030307	biological_process	positive regulation of cell growth
A	0030335	biological_process	positive regulation of cell migration
A	0031398	biological_process	positive regulation of protein ubiquitination
A	0031410	cellular_component	cytoplasmic vesicle
A	0031901	cellular_component	early endosome membrane
A	0032740	biological_process	positive regulation of interleukin-17 production
A	0034248	biological_process	regulation of amide metabolic process
A	0034612	biological_process	response to tumor necrosis factor
A	0035556	biological_process	intracellular signal transduction
A	0035924	biological_process	cellular response to vascular endothelial growth factor stimulus
A	0038036	molecular_function	sphingosine-1-phosphate receptor activity
A	0043066	biological_process	negative regulation of apoptotic process
A	0045202	cellular_component	synapse
A	0045766	biological_process	positive regulation of angiogenesis
A	0045931	biological_process	positive regulation of mitotic cell cycle
A	0045987	biological_process	positive regulation of smooth muscle contraction
A	0046512	biological_process	sphingosine biosynthetic process
A	0046521	biological_process	sphingoid catabolic process
A	0046890	biological_process	regulation of lipid biosynthetic process
A	0048146	biological_process	positive regulation of fibroblast proliferation
A	0050764	biological_process	regulation of phagocytosis
A	0051049	biological_process	regulation of transport
A	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
A	0051239	biological_process	regulation of multicellular organismal process
A	0051721	molecular_function	protein phosphatase 2A binding
A	0071363	biological_process	cellular response to growth factor stimulus
A	0098793	cellular_component	presynapse
A	0150077	biological_process	regulation of neuroinflammatory response
A	1900060	biological_process	negative regulation of ceramide biosynthetic process
A	1900745	biological_process	positive regulation of p38MAPK cascade
A	1901224	biological_process	positive regulation of non-canonical NF-kappaB signal transduction
A	1903978	biological_process	regulation of microglial cell activation
A	1905364	biological_process	regulation of endosomal vesicle fusion
B	0000166	molecular_function	nucleotide binding
B	0000287	molecular_function	magnesium ion binding
B	0001727	molecular_function	lipid kinase activity
B	0003376	biological_process	sphingosine-1-phosphate receptor signaling pathway
B	0003677	molecular_function	DNA binding
B	0005515	molecular_function	protein binding
B	0005516	molecular_function	calmodulin binding
B	0005524	molecular_function	ATP binding
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0005886	cellular_component	plasma membrane
B	0005905	cellular_component	clathrin-coated pit
B	0006473	biological_process	protein acetylation
B	0006629	biological_process	lipid metabolic process
B	0006670	biological_process	sphingosine metabolic process
B	0008289	molecular_function	lipid binding
B	0008481	molecular_function	sphingosine kinase activity
B	0010008	cellular_component	endosome membrane
B	0010468	biological_process	regulation of gene expression
B	0010803	biological_process	regulation of tumor necrosis factor-mediated signaling pathway
B	0016301	molecular_function	kinase activity
B	0016407	molecular_function	acetyltransferase activity
B	0016740	molecular_function	transferase activity
B	0019722	biological_process	calcium-mediated signaling
B	0030100	biological_process	regulation of endocytosis
B	0030139	cellular_component	endocytic vesicle
B	0030148	biological_process	sphingolipid biosynthetic process
B	0030307	biological_process	positive regulation of cell growth
B	0030335	biological_process	positive regulation of cell migration
B	0031398	biological_process	positive regulation of protein ubiquitination
B	0031410	cellular_component	cytoplasmic vesicle
B	0031901	cellular_component	early endosome membrane
B	0032740	biological_process	positive regulation of interleukin-17 production
B	0034248	biological_process	regulation of amide metabolic process
B	0034612	biological_process	response to tumor necrosis factor
B	0035556	biological_process	intracellular signal transduction
B	0035924	biological_process	cellular response to vascular endothelial growth factor stimulus
B	0038036	molecular_function	sphingosine-1-phosphate receptor activity
B	0043066	biological_process	negative regulation of apoptotic process
B	0045202	cellular_component	synapse
B	0045766	biological_process	positive regulation of angiogenesis
B	0045931	biological_process	positive regulation of mitotic cell cycle
B	0045987	biological_process	positive regulation of smooth muscle contraction
B	0046512	biological_process	sphingosine biosynthetic process
B	0046521	biological_process	sphingoid catabolic process
B	0046890	biological_process	regulation of lipid biosynthetic process
B	0048146	biological_process	positive regulation of fibroblast proliferation
B	0050764	biological_process	regulation of phagocytosis
B	0051049	biological_process	regulation of transport
B	0051092	biological_process	positive regulation of NF-kappaB transcription factor activity
B	0051239	biological_process	regulation of multicellular organismal process
B	0051721	molecular_function	protein phosphatase 2A binding
B	0071363	biological_process	cellular response to growth factor stimulus
B	0098793	cellular_component	presynapse
B	0150077	biological_process	regulation of neuroinflammatory response
B	1900060	biological_process	negative regulation of ceramide biosynthetic process
B	1900745	biological_process	positive regulation of p38MAPK cascade
B	1901224	biological_process	positive regulation of non-canonical NF-kappaB signal transduction
B	1903978	biological_process	regulation of microglial cell activation
B	1905364	biological_process	regulation of endosomal vesicle fusion

Functional Information from PDB Data

site_id	AC1
Number of Residues	12
Details	BINDING SITE FOR RESIDUE GYR A 1450

Chain	Residue
A	PHE259
A	HIS397
A	LEU405
A	HOH2123
A	ILE260
A	VAL263
A	ASP264
A	THR282
A	MET358
A	ALA360
A	PHE374
A	LEU388

site_id	AC2
Number of Residues	14
Details	BINDING SITE FOR RESIDUE GYR B 1450

Chain	Residue
B	PHE259
B	ILE260
B	VAL263
B	ASP264
B	THR282
B	LEU347
B	MET358
B	ALA360
B	PHE374
B	LEU388
B	MET392
B	HIS397
B	LEU405
B	HOH2140

site_id	AC3
Number of Residues	1
Details	BINDING SITE FOR RESIDUE ACT A 1451

Chain	Residue
A	HOH2229

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	147
Details	Domain: {"description":"DAGKc","evidences":[{"source":"PROSITE-ProRule","id":"PRU00783","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	16
Details	Motif: {"description":"Nuclear export signal 1","evidences":[{"source":"PubMed","id":"14575709","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	16
Details	Motif: {"description":"Nuclear export signal 2","evidences":[{"source":"PubMed","id":"14575709","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	2
Details	Active site: {"description":"Proton donor/acceptor","evidences":[{"source":"PubMed","id":"23602659","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	32
Details	Binding site: {"evidences":[{"source":"PubMed","id":"23602659","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"20577214","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)