4NIF

Heterodimeric structure of ERK2 and RSK1

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0004672	molecular_function	protein kinase activity
A	0005524	molecular_function	ATP binding
A	0006468	biological_process	protein phosphorylation
B	0000165	biological_process	MAPK cascade
B	0000166	molecular_function	nucleotide binding
B	0001784	molecular_function	phosphotyrosine residue binding
B	0003677	molecular_function	DNA binding
B	0004672	molecular_function	protein kinase activity
B	0004674	molecular_function	protein serine/threonine kinase activity
B	0004707	molecular_function	MAP kinase activity
B	0005515	molecular_function	protein binding
B	0005524	molecular_function	ATP binding
B	0005576	cellular_component	extracellular region
B	0005634	cellular_component	nucleus
B	0005654	cellular_component	nucleoplasm
B	0005737	cellular_component	cytoplasm
B	0005739	cellular_component	mitochondrion
B	0005769	cellular_component	early endosome
B	0005770	cellular_component	late endosome
B	0005788	cellular_component	endoplasmic reticulum lumen
B	0005794	cellular_component	Golgi apparatus
B	0005813	cellular_component	centrosome
B	0005819	cellular_component	spindle
B	0005829	cellular_component	cytosol
B	0005856	cellular_component	cytoskeleton
B	0005886	cellular_component	plasma membrane
B	0005901	cellular_component	caveola
B	0005925	cellular_component	focal adhesion
B	0005929	cellular_component	cilium
B	0006357	biological_process	regulation of transcription by RNA polymerase II
B	0006468	biological_process	protein phosphorylation
B	0006915	biological_process	apoptotic process
B	0006935	biological_process	chemotaxis
B	0006974	biological_process	DNA damage response
B	0007165	biological_process	signal transduction
B	0007166	biological_process	cell surface receptor signaling pathway
B	0007173	biological_process	epidermal growth factor receptor signaling pathway
B	0007268	biological_process	chemical synaptic transmission
B	0007507	biological_process	heart development
B	0007611	biological_process	learning or memory
B	0008286	biological_process	insulin receptor signaling pathway
B	0008353	molecular_function	RNA polymerase II CTD heptapeptide repeat kinase activity
B	0009887	biological_process	animal organ morphogenesis
B	0010759	biological_process	positive regulation of macrophage chemotaxis
B	0010800	biological_process	positive regulation of peptidyl-threonine phosphorylation
B	0014032	biological_process	neural crest cell development
B	0014044	biological_process	Schwann cell development
B	0015630	cellular_component	microtubule cytoskeleton
B	0016020	cellular_component	membrane
B	0016301	molecular_function	kinase activity
B	0016740	molecular_function	transferase activity
B	0018107	biological_process	peptidyl-threonine phosphorylation
B	0019858	biological_process	cytosine metabolic process
B	0019902	molecular_function	phosphatase binding
B	0030278	biological_process	regulation of ossification
B	0030641	biological_process	regulation of cellular pH
B	0030878	biological_process	thyroid gland development
B	0031143	cellular_component	pseudopodium
B	0031647	biological_process	regulation of protein stability
B	0031663	biological_process	lipopolysaccharide-mediated signaling pathway
B	0032206	biological_process	positive regulation of telomere maintenance
B	0032496	biological_process	response to lipopolysaccharide
B	0032872	biological_process	regulation of stress-activated MAPK cascade
B	0033598	biological_process	mammary gland epithelial cell proliferation
B	0034198	biological_process	cellular response to amino acid starvation
B	0035094	biological_process	response to nicotine
B	0035556	biological_process	intracellular signal transduction
B	0035578	cellular_component	azurophil granule lumen
B	0036064	cellular_component	ciliary basal body
B	0038127	biological_process	ERBB signaling pathway
B	0038133	biological_process	ERBB2-ERBB3 signaling pathway
B	0042473	biological_process	outer ear morphogenesis
B	0042552	biological_process	myelination
B	0042802	molecular_function	identical protein binding
B	0043330	biological_process	response to exogenous dsRNA
B	0045202	cellular_component	synapse
B	0045542	biological_process	positive regulation of cholesterol biosynthetic process
B	0045596	biological_process	negative regulation of cell differentiation
B	0048009	biological_process	insulin-like growth factor receptor signaling pathway
B	0048538	biological_process	thymus development
B	0050852	biological_process	T cell receptor signaling pathway
B	0050853	biological_process	B cell receptor signaling pathway
B	0051403	biological_process	stress-activated MAPK cascade
B	0051493	biological_process	regulation of cytoskeleton organization
B	0060020	biological_process	Bergmann glial cell differentiation
B	0060291	biological_process	long-term synaptic potentiation
B	0060324	biological_process	face development
B	0060425	biological_process	lung morphogenesis
B	0060440	biological_process	trachea formation
B	0060716	biological_process	labyrinthine layer blood vessel development
B	0061308	biological_process	cardiac neural crest cell development involved in heart development
B	0061514	biological_process	interleukin-34-mediated signaling pathway
B	0070098	biological_process	chemokine-mediated signaling pathway
B	0070161	cellular_component	anchoring junction
B	0070371	biological_process	ERK1 and ERK2 cascade
B	0070849	biological_process	response to epidermal growth factor
B	0071356	biological_process	cellular response to tumor necrosis factor
B	0072584	biological_process	caveolin-mediated endocytosis
B	0072686	cellular_component	mitotic spindle
B	0090170	biological_process	regulation of Golgi inheritance
B	0106310	molecular_function	protein serine kinase activity
B	0120041	biological_process	positive regulation of macrophage proliferation
B	0150078	biological_process	positive regulation of neuroinflammatory response
B	1904813	cellular_component	ficolin-1-rich granule lumen
B	2000641	biological_process	regulation of early endosome to late endosome transport
D	0004672	molecular_function	protein kinase activity
D	0005524	molecular_function	ATP binding
D	0006468	biological_process	protein phosphorylation
E	0000165	biological_process	MAPK cascade
E	0000166	molecular_function	nucleotide binding
E	0001784	molecular_function	phosphotyrosine residue binding
E	0003677	molecular_function	DNA binding
E	0004672	molecular_function	protein kinase activity
E	0004674	molecular_function	protein serine/threonine kinase activity
E	0004707	molecular_function	MAP kinase activity
E	0005515	molecular_function	protein binding
E	0005524	molecular_function	ATP binding
E	0005576	cellular_component	extracellular region
E	0005634	cellular_component	nucleus
E	0005654	cellular_component	nucleoplasm
E	0005737	cellular_component	cytoplasm
E	0005739	cellular_component	mitochondrion
E	0005769	cellular_component	early endosome
E	0005770	cellular_component	late endosome
E	0005788	cellular_component	endoplasmic reticulum lumen
E	0005794	cellular_component	Golgi apparatus
E	0005813	cellular_component	centrosome
E	0005819	cellular_component	spindle
E	0005829	cellular_component	cytosol
E	0005856	cellular_component	cytoskeleton
E	0005886	cellular_component	plasma membrane
E	0005901	cellular_component	caveola
E	0005925	cellular_component	focal adhesion
E	0005929	cellular_component	cilium
E	0006357	biological_process	regulation of transcription by RNA polymerase II
E	0006468	biological_process	protein phosphorylation
E	0006915	biological_process	apoptotic process
E	0006935	biological_process	chemotaxis
E	0006974	biological_process	DNA damage response
E	0007165	biological_process	signal transduction
E	0007166	biological_process	cell surface receptor signaling pathway
E	0007173	biological_process	epidermal growth factor receptor signaling pathway
E	0007268	biological_process	chemical synaptic transmission
E	0007507	biological_process	heart development
E	0007611	biological_process	learning or memory
E	0008286	biological_process	insulin receptor signaling pathway
E	0008353	molecular_function	RNA polymerase II CTD heptapeptide repeat kinase activity
E	0009887	biological_process	animal organ morphogenesis
E	0010759	biological_process	positive regulation of macrophage chemotaxis
E	0010800	biological_process	positive regulation of peptidyl-threonine phosphorylation
E	0014032	biological_process	neural crest cell development
E	0014044	biological_process	Schwann cell development
E	0015630	cellular_component	microtubule cytoskeleton
E	0016020	cellular_component	membrane
E	0016301	molecular_function	kinase activity
E	0016740	molecular_function	transferase activity
E	0018107	biological_process	peptidyl-threonine phosphorylation
E	0019858	biological_process	cytosine metabolic process
E	0019902	molecular_function	phosphatase binding
E	0030278	biological_process	regulation of ossification
E	0030641	biological_process	regulation of cellular pH
E	0030878	biological_process	thyroid gland development
E	0031143	cellular_component	pseudopodium
E	0031647	biological_process	regulation of protein stability
E	0031663	biological_process	lipopolysaccharide-mediated signaling pathway
E	0032206	biological_process	positive regulation of telomere maintenance
E	0032496	biological_process	response to lipopolysaccharide
E	0032872	biological_process	regulation of stress-activated MAPK cascade
E	0033598	biological_process	mammary gland epithelial cell proliferation
E	0034198	biological_process	cellular response to amino acid starvation
E	0035094	biological_process	response to nicotine
E	0035556	biological_process	intracellular signal transduction
E	0035578	cellular_component	azurophil granule lumen
E	0036064	cellular_component	ciliary basal body
E	0038127	biological_process	ERBB signaling pathway
E	0038133	biological_process	ERBB2-ERBB3 signaling pathway
E	0042473	biological_process	outer ear morphogenesis
E	0042552	biological_process	myelination
E	0042802	molecular_function	identical protein binding
E	0043330	biological_process	response to exogenous dsRNA
E	0045202	cellular_component	synapse
E	0045542	biological_process	positive regulation of cholesterol biosynthetic process
E	0045596	biological_process	negative regulation of cell differentiation
E	0048009	biological_process	insulin-like growth factor receptor signaling pathway
E	0048538	biological_process	thymus development
E	0050852	biological_process	T cell receptor signaling pathway
E	0050853	biological_process	B cell receptor signaling pathway
E	0051403	biological_process	stress-activated MAPK cascade
E	0051493	biological_process	regulation of cytoskeleton organization
E	0060020	biological_process	Bergmann glial cell differentiation
E	0060291	biological_process	long-term synaptic potentiation
E	0060324	biological_process	face development
E	0060425	biological_process	lung morphogenesis
E	0060440	biological_process	trachea formation
E	0060716	biological_process	labyrinthine layer blood vessel development
E	0061308	biological_process	cardiac neural crest cell development involved in heart development
E	0061514	biological_process	interleukin-34-mediated signaling pathway
E	0070098	biological_process	chemokine-mediated signaling pathway
E	0070161	cellular_component	anchoring junction
E	0070371	biological_process	ERK1 and ERK2 cascade
E	0070849	biological_process	response to epidermal growth factor
E	0071356	biological_process	cellular response to tumor necrosis factor
E	0072584	biological_process	caveolin-mediated endocytosis
E	0072686	cellular_component	mitotic spindle
E	0090170	biological_process	regulation of Golgi inheritance
E	0106310	molecular_function	protein serine kinase activity
E	0120041	biological_process	positive regulation of macrophage proliferation
E	0150078	biological_process	positive regulation of neuroinflammatory response
E	1904813	cellular_component	ficolin-1-rich granule lumen
E	2000641	biological_process	regulation of early endosome to late endosome transport

Functional Information from PDB Data

site_id	AC1
Number of Residues	24
Details	BINDING SITE FOR RESIDUE ANP B 401

Chain	Residue
B	GLY34
B	ASP106
B	MET108
B	ASP111
B	LYS114
B	LYS151
B	SER153
B	ASP167
B	HOH509
B	HOH514
B	HOH537
B	ALA35
B	HOH538
B	HOH548
B	HOH623
B	HOH646
B	HOH806
B	TYR36
B	GLY37
B	VAL39
B	ALA52
B	LYS54
B	ARG67
B	GLN105

---

site_id	AC2
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SO4 B 402

Chain	Residue
A	ASN568
B	ARG191
B	HIS232
B	TYR233
B	HOH539
B	HOH713
D	SER415
D	TYR479
D	HOH844

---

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE SO4 B 403

Chain	Residue
B	HIS61
B	GLN62
B	LYS340
B	HOH789

---

site_id	AC4
Number of Residues	4
Details	BINDING SITE FOR RESIDUE NA B 404

Chain	Residue
B	ARG70
B	VAL173
B	HOH515
B	HOH738

---

site_id	AC5
Number of Residues	24
Details	BINDING SITE FOR RESIDUE ANP E 401

Chain	Residue
E	GLU33
E	GLY34
E	ALA35
E	TYR36
E	GLY37
E	VAL39
E	ALA52
E	LYS54
E	ARG67
E	GLN105
E	ASP106
E	MET108
E	ASP111
E	LYS151
E	SER153
E	LEU156
E	ASP167
E	HOH506
E	HOH510
E	HOH513
E	HOH539
E	HOH560
E	HOH736
E	HOH773

---

site_id	AC6
Number of Residues	9
Details	BINDING SITE FOR RESIDUE SO4 E 402

Chain	Residue
A	SER415
A	TYR479
A	HOH928
D	ASN568
E	ARG191
E	HIS232
E	TYR233
E	HOH526
E	HOH763

---

site_id	AC7
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NA E 403

Chain	Residue
E	ARG70
E	VAL173
E	PHE331
E	HOH524
E	HOH796

---

Functional Information from PROSITE/UniProt

site_id	PS00107
Number of Residues	24
Details	PROTEIN_KINASE_ATP Protein kinases ATP-binding region signature. IGVGSYSECKrCvhkatnme..........YAVK

Chain	Residue	Details
A	ILE424-LYS447
B	ILE31-LYS55

---

site_id	PS00108
Number of Residues	13
Details	PROTEIN_KINASE_ST Serine/Threonine protein kinases active-site signature. VvHrDLKpsNILY

Chain	Residue	Details
A	VAL531-TYR543
B	VAL145-LEU157

---

site_id	PS01351
Number of Residues	103
Details	MAPK MAP kinase signature. FehqtycqrtlREikillrfrheniigindiiraptieqmkdvyivqdlmetdlykllktqhlsndhicyflyqilrglkyihsanvlh..........RDlKpsnlllnttC

Chain	Residue	Details
B	PHE59-CYS161

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	Active site: {"description":"Proton acceptor","evidences":[{"evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	36
Details	Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"9430688","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	36
Details	DNA binding: {}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	4
Details	Motif: {"description":"TXY","evidences":[{"source":"PubMed","id":"18760948","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	8
Details	Motif: {"description":"Cytoplasmic retention motif","evidences":[{"source":"PubMed","id":"18760948","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	12
Details	Motif: {"description":"Nuclear translocation motif","evidences":[{"source":"PubMed","id":"18760948","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Active site: {"description":"Proton acceptor","evidences":[{"source":"PROSITE-ProRule","id":"PRU00159","evidenceCode":"ECO:0000255"},{"source":"PROSITE-ProRule","id":"PRU10027","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine; by SGK1","evidences":[{"source":"PubMed","id":"19447520","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine; by MAP2K1 and MAP2K2","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"19690332","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"21406692","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI11
Number of Residues	2
Details	Modified residue: {"description":"Phosphotyrosine; by MAP2K1 and MAP2K2","evidences":[{"source":"PubMed","id":"19053285","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19494114","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"19690332","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"21406692","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI12
Number of Residues	2
Details	Modified residue: {"description":"Phosphothreonine; by autocatalysis","evidences":[{"source":"PubMed","id":"19060905","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI13
Number of Residues	4
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"18760948","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI14
Number of Residues	2
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"19369195","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---