4NCB

Structure of Thermus thermophilus Argonaute bound to guide DNA and 19-mer target DNA with Mg2+

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003676	molecular_function	nucleic acid binding
A	0003677	molecular_function	DNA binding
A	0003723	molecular_function	RNA binding
A	0004518	molecular_function	nuclease activity
A	0004519	molecular_function	endonuclease activity
A	0004520	molecular_function	DNA endonuclease activity
A	0006260	biological_process	DNA replication
A	0016787	molecular_function	hydrolase activity
A	0030145	molecular_function	manganese ion binding
A	0044355	biological_process	clearance of foreign intracellular DNA
A	0046872	molecular_function	metal ion binding
B	0003676	molecular_function	nucleic acid binding
B	0003677	molecular_function	DNA binding
B	0003723	molecular_function	RNA binding
B	0004518	molecular_function	nuclease activity
B	0004519	molecular_function	endonuclease activity
B	0004520	molecular_function	DNA endonuclease activity
B	0006260	biological_process	DNA replication
B	0016787	molecular_function	hydrolase activity
B	0030145	molecular_function	manganese ion binding
B	0044355	biological_process	clearance of foreign intracellular DNA
B	0046872	molecular_function	metal ion binding

Functional Information from PDB Data

site_id	AC1
Number of Residues	14
Details	BINDING SITE FOR RESIDUE TMP A 701

Chain	Residue
A	ASN195
A	ILE254
A	PRO255
A	HIS256
B	PRO37
B	ARG39
A	TYR197
A	ARG200
A	LEU217
A	PRO218
A	LEU223
A	TYR226
A	HIS227
A	ARG232

---

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A 702

Chain	Residue
A	ASP478
A	ASP660
D	DT10
D	HOH201
D	HOH202
D	HOH203

---

site_id	AC3
Number of Residues	16
Details	BINDING SITE FOR RESIDUE TMP B 701

Chain	Residue
A	PRO37
A	ARG39
A	VAL42
B	ASN195
B	TYR197
B	ARG200
B	TRP202
B	LEU217
B	PRO218
B	LEU223
B	TYR226
B	HIS227
B	ARG232
B	ILE254
B	PRO255
B	HIS256

---

site_id	AC4
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG B 702

Chain	Residue
B	ASP478
B	ASP660
B	MG703
B	HOH802
B	HOH958
F	DT10

---

site_id	AC5
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG B 703

Chain	Residue
B	ASP478
B	ASP546
B	MG702
B	HOH803
B	HOH804
F	DT10
H	DC9

---

site_id	AC6
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG C 101

Chain	Residue
A	VAL685
A	HOH803
C	DT1
C	DA3
C	HOH221

---

site_id	AC7
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG D 101

Chain	Residue
A	ASP478
A	ASP546
A	HOH801
A	HOH802
D	DC9
D	DT10
P	DC9

---

site_id	AC8
Number of Residues	5
Details	BINDING SITE FOR RESIDUE MG E 101

Chain	Residue
B	VAL685
E	DT1
E	DA3
E	HOH202
E	HOH208

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	96
Details	Domain: {"description":"PAZ","evidences":[{"source":"PROSITE-ProRule","id":"PRU00142","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"18754009","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19092929","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI2
Number of Residues	328
Details	Domain: {"description":"Piwi","evidences":[{"source":"PROSITE-ProRule","id":"PRU00150","evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI3
Number of Residues	152
Details	Region: {"description":"Linker L1","evidences":[{"source":"PubMed","id":"18754009","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19092929","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI4
Number of Residues	250
Details	Region: {"description":"Mid domain","evidences":[{"source":"PubMed","id":"18754009","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19092929","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI5
Number of Residues	442
Details	Region: {"description":"PIWI domain","evidences":[{"source":"PubMed","id":"18754009","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19092929","evidenceCode":"ECO:0000269"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI6
Number of Residues	6
Details	Active site: {"evidences":[{"source":"PubMed","id":"18754009","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19092929","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"24374628","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"28911094","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI7
Number of Residues	2
Details	Active site: {"evidences":[{"source":"PubMed","id":"24374628","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"28911094","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI8
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"24374628","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19812667","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"28911094","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"4KPY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N76","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI9
Number of Residues	4
Details	Binding site: {"evidences":[{"source":"PubMed","id":"24374628","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19092929","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19812667","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"28911094","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"4KPY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N76","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---

site_id	SWS_FT_FI10
Number of Residues	2
Details	Binding site: {"evidences":[{"source":"PubMed","id":"24374628","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18754009","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19092929","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19812667","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"28911094","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"4KPY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N76","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

---