4N76
Structure of Thermus thermophilus Argonaute bound to guide DNA and cleaved target DNA with Mn2+
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003676 | molecular_function | nucleic acid binding |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003723 | molecular_function | RNA binding |
| A | 0004518 | molecular_function | nuclease activity |
| A | 0004519 | molecular_function | endonuclease activity |
| A | 0004520 | molecular_function | DNA endonuclease activity |
| A | 0006260 | biological_process | DNA replication |
| A | 0016787 | molecular_function | hydrolase activity |
| A | 0030145 | molecular_function | manganese ion binding |
| A | 0044355 | biological_process | clearance of foreign intracellular DNA |
| A | 0046872 | molecular_function | metal ion binding |
| B | 0003676 | molecular_function | nucleic acid binding |
| B | 0003677 | molecular_function | DNA binding |
| B | 0003723 | molecular_function | RNA binding |
| B | 0004518 | molecular_function | nuclease activity |
| B | 0004519 | molecular_function | endonuclease activity |
| B | 0004520 | molecular_function | DNA endonuclease activity |
| B | 0006260 | biological_process | DNA replication |
| B | 0016787 | molecular_function | hydrolase activity |
| B | 0030145 | molecular_function | manganese ion binding |
| B | 0044355 | biological_process | clearance of foreign intracellular DNA |
| B | 0046872 | molecular_function | metal ion binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MN B 701 |
| Chain | Residue |
| B | GLN433 |
| B | LYS457 |
| B | VAL685 |
| E | DT1 |
| E | DA3 |
| site_id | AC2 |
| Number of Residues | 6 |
| Details | BINDING SITE FOR RESIDUE MN B 702 |
| Chain | Residue |
| B | MN703 |
| F | DT10 |
| B | ASP478 |
| B | ALA479 |
| B | GLU512 |
| B | ASP546 |
| site_id | AC3 |
| Number of Residues | 4 |
| Details | BINDING SITE FOR RESIDUE MN B 703 |
| Chain | Residue |
| B | ASP478 |
| B | ASP660 |
| B | MN702 |
| F | DT10 |
| site_id | AC4 |
| Number of Residues | 11 |
| Details | BINDING SITE FOR RESIDUE TMP B 704 |
| Chain | Residue |
| A | PRO37 |
| A | ARG39 |
| A | VAL42 |
| B | ASN195 |
| B | TYR197 |
| B | PRO218 |
| B | TYR226 |
| B | HIS227 |
| B | ARG232 |
| B | PRO255 |
| B | HIS256 |
| site_id | AC5 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE MN C 101 |
| Chain | Residue |
| A | LYS457 |
| A | VAL685 |
| C | DT1 |
| C | DA3 |
| C | HOH201 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 328 |
| Details | Domain: {"description":"Piwi","evidences":[{"source":"PROSITE-ProRule","id":"PRU00150","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 152 |
| Details | Region: {"description":"Linker L1","evidences":[{"source":"PubMed","id":"18754009","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19092929","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 250 |
| Details | Region: {"description":"Mid domain","evidences":[{"source":"PubMed","id":"18754009","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19092929","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 442 |
| Details | Region: {"description":"PIWI domain","evidences":[{"source":"PubMed","id":"18754009","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19092929","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 6 |
| Details | Active site: {"evidences":[{"source":"PubMed","id":"18754009","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19092929","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"24374628","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"28911094","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 2 |
| Details | Active site: {"evidences":[{"source":"PubMed","id":"24374628","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"28911094","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"24374628","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19812667","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"28911094","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"4KPY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N76","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 4 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"24374628","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19092929","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19812667","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"28911094","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"4KPY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N76","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"24374628","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"18754009","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19092929","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"19812667","evidenceCode":"ECO:0000305"},{"source":"PubMed","id":"28911094","evidenceCode":"ECO:0000305"},{"source":"PDB","id":"4KPY","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"4N76","evidenceCode":"ECO:0007744"}]} |
| Chain | Residue | Details |
