4N76
Structure of Thermus thermophilus Argonaute bound to guide DNA and cleaved target DNA with Mn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-15 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 110.918, 117.420, 160.457 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.372 - 2.890 |
R-factor | 0.2215 |
Rwork | 0.219 |
R-free | 0.26870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4n47 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.107 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2_1309) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.000 |
High resolution limit [Å] | 2.890 | 2.890 |
Rmerge | 0.160 | |
Number of reflections | 45264 | |
Completeness [%] | 95.5 | 82.2 |
Redundancy | 4.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 306 | 2.8 M sodium acetate, 0.1 M Bis-Tris propane, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 306K |