4GFH
Topoisomerase II-DNA-AMPPNP complex
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0003677 | molecular_function | DNA binding |
| A | 0003918 | molecular_function | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity |
| A | 0005524 | molecular_function | ATP binding |
| A | 0006259 | biological_process | DNA metabolic process |
| A | 0006265 | biological_process | DNA topological change |
| F | 0003677 | molecular_function | DNA binding |
| F | 0003918 | molecular_function | DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity |
| F | 0005524 | molecular_function | ATP binding |
| F | 0006259 | biological_process | DNA metabolic process |
| F | 0006265 | biological_process | DNA topological change |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE MG A 1201 |
| Chain | Residue |
| A | GLU66 |
| A | ASN70 |
| A | ANP1202 |
| site_id | AC2 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE ANP A 1202 |
| Chain | Residue |
| A | PHE121 |
| A | THR126 |
| A | SER127 |
| A | SER128 |
| A | ASN129 |
| A | GLY140 |
| A | ARG141 |
| A | ASN142 |
| A | GLY143 |
| A | TYR144 |
| A | GLY145 |
| A | ALA146 |
| A | LYS147 |
| A | THR195 |
| A | GLN365 |
| A | LYS367 |
| A | MG1201 |
| A | ASN70 |
| A | ASN74 |
| A | ARG77 |
| A | ASN99 |
| A | ILE104 |
| A | ILE120 |
| site_id | AC3 |
| Number of Residues | 3 |
| Details | BINDING SITE FOR RESIDUE MG F 1201 |
| Chain | Residue |
| F | GLU66 |
| F | ASN70 |
| F | ANP1202 |
| site_id | AC4 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE ANP F 1202 |
| Chain | Residue |
| F | ASN70 |
| F | ASN74 |
| F | ARG77 |
| F | ASN99 |
| F | ILE104 |
| F | ILE120 |
| F | PHE121 |
| F | THR126 |
| F | SER127 |
| F | SER128 |
| F | ASN129 |
| F | GLY140 |
| F | ARG141 |
| F | ASN142 |
| F | GLY143 |
| F | TYR144 |
| F | GLY145 |
| F | ALA146 |
| F | LYS147 |
| F | THR195 |
| F | GLN365 |
| F | LYS367 |
| F | MG1201 |
Functional Information from PROSITE/UniProt
| site_id | PS00177 |
| Number of Residues | 9 |
| Details | TOPOISOMERASE_II DNA topoisomerase II signature. LTEGDSALS |
| Chain | Residue | Details |
| A | LEU447-SER455 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 228 |
| Details | Domain: {"description":"Toprim","evidences":[{"source":"PROSITE-ProRule","id":"PRU00995","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 6 |
| Details | Region: {"description":"Interaction with DNA","evidences":[{"source":"PubMed","id":"23022727","evidenceCode":"ECO:0000305"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 18 |
| Details | Region: {"description":"Interaction with DNA","evidences":[{"source":"PubMed","id":"20485342","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 2 |
| Details | Active site: {"description":"O-(5'-phospho-DNA)-tyrosine intermediate","evidences":[{"source":"PROSITE-ProRule","id":"PRU01384","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"20485342","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"9685374","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 26 |
| Details | Binding site: {"evidences":[{"source":"PubMed","id":"12963818","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 6 |
| Details | Binding site: {"evidences":[{"source":"PROSITE-ProRule","id":"PRU00995","evidenceCode":"ECO:0000255"},{"source":"PubMed","id":"18097402","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 8 |
| Details | Site: {"description":"Interaction with DNA","evidences":[{"source":"PROSITE-ProRule","id":"PRU00995","evidenceCode":"ECO:0000255"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI8 |
| Number of Residues | 8 |
| Details | Site: {"description":"Interaction with DNA","evidences":[{"source":"PubMed","id":"20485342","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI9 |
| Number of Residues | 2 |
| Details | Site: {"description":"Transition state stabilizer","evidences":[{"source":"PubMed","id":"20485342","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI10 |
| Number of Residues | 2 |
| Details | Site: {"description":"Important for DNA bending; intercalates between base pairs of target DNA","evidences":[{"source":"PubMed","id":"18097402","evidenceCode":"ECO:0000269"}]} |
| Chain | Residue | Details |
