4GFH

Topoisomerase II-DNA-AMPPNP complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2010-08-21
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.1159
Spacegroup name	P 21 21 21
Unit cell lengths	169.135, 169.884, 169.212
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.988 - 4.408
R-factor	0.241
Rwork	0.239
R-free	0.27540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.842
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.3_928))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	4.570
High resolution limit [Å]	4.408	4.408
Number of reflections	28660
<I/σ(I)>		2
Completeness [%]	91.7	92.9
Redundancy		4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	23% PEG 300, 100 mM Tris (8.0), VAPOR DIFFUSION, HANGING DROP, temperature 291K