4FXY

Crystal structure of rat neurolysin with bound pyrazolidin inhibitor

Functional Information from GO Data

Chain	GOid	namespace	contents
P	0004222	molecular_function	metalloendopeptidase activity
P	0005737	cellular_component	cytoplasm
P	0005739	cellular_component	mitochondrion
P	0005758	cellular_component	mitochondrial intermembrane space
P	0005829	cellular_component	cytosol
P	0005886	cellular_component	plasma membrane
P	0006111	biological_process	regulation of gluconeogenesis
P	0006508	biological_process	proteolysis
P	0006518	biological_process	peptide metabolic process
P	0008233	molecular_function	peptidase activity
P	0008237	molecular_function	metallopeptidase activity
P	0030163	biological_process	protein catabolic process
P	0042277	molecular_function	peptide binding
P	0043171	biological_process	peptide catabolic process
P	0046872	molecular_function	metal ion binding
P	0070012	molecular_function	oligopeptidase activity
P	1902809	biological_process	regulation of skeletal muscle fiber differentiation
Q	0004222	molecular_function	metalloendopeptidase activity
Q	0005737	cellular_component	cytoplasm
Q	0005739	cellular_component	mitochondrion
Q	0005758	cellular_component	mitochondrial intermembrane space
Q	0005829	cellular_component	cytosol
Q	0005886	cellular_component	plasma membrane
Q	0006111	biological_process	regulation of gluconeogenesis
Q	0006508	biological_process	proteolysis
Q	0006518	biological_process	peptide metabolic process
Q	0008233	molecular_function	peptidase activity
Q	0008237	molecular_function	metallopeptidase activity
Q	0030163	biological_process	protein catabolic process
Q	0042277	molecular_function	peptide binding
Q	0043171	biological_process	peptide catabolic process
Q	0046872	molecular_function	metal ion binding
Q	0070012	molecular_function	oligopeptidase activity
Q	1902809	biological_process	regulation of skeletal muscle fiber differentiation

Functional Information from PDB Data

site_id	AC1
Number of Residues	15
Details	BINDING SITE FOR RESIDUE 0W2 P 701

Chain	Residue
P	THR45
P	ILE123
P	SER146
P	ARG491
P	HOH837
P	HOH838
P	HOH927
P	TYR49
P	LEU69
P	ILE72
P	GLU73
P	TYR76
P	ARG80
P	ASP110
P	MET113

---

site_id	AC2
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN P 702

Chain	Residue
P	HIS474
P	GLU475
P	HIS478
P	GLU503
P	HOH843

---

site_id	AC3
Number of Residues	16
Details	BINDING SITE FOR RESIDUE 0W2 Q 701

Chain	Residue
Q	THR45
Q	TYR49
Q	LEU69
Q	ILE72
Q	GLU73
Q	TYR76
Q	ARG80
Q	ASP110
Q	MET113
Q	ARG116
Q	ILE123
Q	SER146
Q	ARG491
Q	HOH928
Q	HOH929
Q	HOH930

---

site_id	AC4
Number of Residues	5
Details	BINDING SITE FOR RESIDUE ZN Q 702

Chain	Residue
Q	HIS474
Q	GLU475
Q	HIS478
Q	GLU503
Q	HOH836

---

Functional Information from PROSITE/UniProt

site_id	PS00142
Number of Residues	10
Details	ZINC_PROTEASE Neutral zinc metallopeptidases, zinc-binding region signature. TYFHEFGHVM

Chain	Residue	Details
P	THR471-MET480

---

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	2
Details	ACT_SITE: ACT_SITE => ECO:0000255|PROSITE-ProRule:PRU10095

Chain	Residue	Details
P	GLU475
Q	GLU475

---

site_id	SWS_FT_FI2
Number of Residues	6
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU10095

Chain	Residue	Details
P	HIS474
P	HIS478
P	HIS481
Q	HIS474
Q	HIS478
Q	HIS481

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	MOD_RES: N6-acetyllysine => ECO:0000250|UniProtKB:Q9BYT8

Chain	Residue	Details
P	LYS641
Q	LYS641

---

Catalytic Information from CSA

site_id	MCSA1
Number of Residues	5
Details	M-CSA 638

Chain	Residue	Details
P	HIS474
P	GLU475
P	HIS478
P	GLU503	proton shuttle (general acid/base)
P	TYR613	electrostatic stabiliser

---

site_id	MCSA2
Number of Residues	5
Details	M-CSA 638

Chain	Residue	Details
Q	HIS474
Q	GLU475
Q	HIS478
Q	GLU503	proton shuttle (general acid/base)
Q	TYR613	electrostatic stabiliser

---