4FM9

Human topoisomerase II alpha bound to DNA

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0003677	molecular_function	DNA binding
A	0003918	molecular_function	DNA topoisomerase type II (double strand cut, ATP-hydrolyzing) activity
A	0005524	molecular_function	ATP binding
A	0006259	biological_process	DNA metabolic process
A	0006265	biological_process	DNA topological change

Functional Information from PDB Data

site_id	AC1
Number of Residues	7
Details	BINDING SITE FOR RESIDUE MG A 1201

Chain	Residue
A	ASP541
A	ASP543
A	LYS614
A	TYR805
A	HOH1301
A	HOH1327
C	HOH202

---

site_id	AC2
Number of Residues	4
Details	BINDING SITE FOR RESIDUE GOL A 1202

Chain	Residue
A	LEU592
A	GLU702
A	SER547
A	SER591

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Functional Information from PROSITE/UniProt

site_id	PS00177
Number of Residues	9
Details	TOPOISOMERASE_II DNA topoisomerase II signature. LTEGDSAKT

Chain	Residue	Details
A	LEU459-THR467

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Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	1
Details	ACT_SITE: O-(5'-phospho-DNA)-tyrosine intermediate => ECO:0000255|PROSITE-ProRule:PRU01384

Chain	Residue	Details
A	TYR805

---

site_id	SWS_FT_FI2
Number of Residues	1
Details	BINDING: BINDING => ECO:0000255|PROSITE-ProRule:PRU00995

Chain	Residue	Details
A	GLU461

---

site_id	SWS_FT_FI3
Number of Residues	2
Details	BINDING: BINDING => ECO:0000269|PubMed:22841979

Chain	Residue	Details
A	ASP541
A	ASP543

---

site_id	SWS_FT_FI4
Number of Residues	1
Details	SITE: Interaction with DNA => ECO:0000255|PROSITE-ProRule:PRU00995

Chain	Residue	Details
A	LYS489

---

site_id	SWS_FT_FI5
Number of Residues	7
Details	SITE: Interaction with DNA => ECO:0000269|PubMed:22841979

Chain	Residue	Details
A	ASN492
A	ARG661
A	LYS662
A	LYS723
A	TYR757
A	SER763
A	TRP931

---

site_id	SWS_FT_FI6
Number of Residues	1
Details	SITE: Transition state stabilizer => ECO:0000250|UniProtKB:P06786

Chain	Residue	Details
A	ARG804

---

site_id	SWS_FT_FI7
Number of Residues	1
Details	SITE: Important for DNA bending; intercalates between base pairs of target DNA => ECO:0000250|UniProtKB:P06786

Chain	Residue	Details
A	ILE856

---

site_id	SWS_FT_FI8
Number of Residues	1
Details	MOD_RES: Phosphoserine; by CK1 => ECO:0000269|PubMed:19043076, ECO:0000269|Ref.11, ECO:0007744|PubMed:17081983, ECO:0007744|PubMed:18669648, ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163

Chain	Residue	Details
A	SER1106

---

site_id	SWS_FT_FI9
Number of Residues	13
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:28112733

Chain	Residue	Details
A	LYS440
A	LYS625
A	LYS632
A	LYS655
A	LYS1114
A	LYS466
A	LYS480
A	LYS529
A	LYS584
A	LYS599
A	LYS614
A	LYS622

---

site_id	SWS_FT_FI10
Number of Residues	4
Details	CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0007744|PubMed:25772364, ECO:0007744|PubMed:28112733

Chain	Residue	Details
A	LYS639
A	LYS662
A	LYS676
A	LYS1075

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