4FEX

Crystal structure of the aminoglycoside phosphotransferase APH(3')-Ia, with substrate kanamycin and small molecule inhibitor tyrphostin AG1478

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0005524	molecular_function	ATP binding
A	0016773	molecular_function	phosphotransferase activity, alcohol group as acceptor
A	0046677	biological_process	response to antibiotic
B	0005524	molecular_function	ATP binding
B	0016773	molecular_function	phosphotransferase activity, alcohol group as acceptor
B	0046677	biological_process	response to antibiotic
C	0005524	molecular_function	ATP binding
C	0016773	molecular_function	phosphotransferase activity, alcohol group as acceptor
C	0046677	biological_process	response to antibiotic
D	0005524	molecular_function	ATP binding
D	0016773	molecular_function	phosphotransferase activity, alcohol group as acceptor
D	0046677	biological_process	response to antibiotic
E	0005524	molecular_function	ATP binding
E	0016773	molecular_function	phosphotransferase activity, alcohol group as acceptor
E	0046677	biological_process	response to antibiotic

Functional Information from PDB Data

site_id	AC1
Number of Residues	12
Details	BINDING SITE FOR RESIDUE KAN A 301

Chain	Residue
A	SER36
A	PHE271
A	HOH430
B	HIS3
A	ASP165
A	PHE166
A	ASP167
A	ASP198
A	ASN234
A	GLU238
A	ASP268
A	GLU269

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site_id	AC2
Number of Residues	12
Details	BINDING SITE FOR RESIDUE 0TO A 302

Chain	Residue
A	ILE40
A	PHE53
A	LYS55
A	GLU68
A	LEU72
A	THR98
A	ALA100
A	ILE101
A	GLY103
A	THR105
A	ASP202
A	ILE215

---

site_id	AC3
Number of Residues	5
Details	BINDING SITE FOR RESIDUE NA A 303

Chain	Residue
A	THR76
A	MET79
A	HOH417
A	HOH433
A	HOH434

---

site_id	AC4
Number of Residues	13
Details	BINDING SITE FOR RESIDUE KAN B 301

Chain	Residue
B	ASP165
B	PHE166
B	ASP167
B	ASP198
B	ASN234
B	CYS235
B	GLU238
B	ASP268
B	GLU269
B	PHE271
B	HOH405
B	HOH409
E	GLY252

---

site_id	AC5
Number of Residues	2
Details	BINDING SITE FOR RESIDUE NA B 302

Chain	Residue
B	THR76
B	MET79

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site_id	AC6
Number of Residues	12
Details	BINDING SITE FOR RESIDUE KAN C 301

Chain	Residue
A	GLY252
C	ASP165
C	ASP167
C	ASP198
C	ASN234
C	CYS235
C	GLU238
C	ASP268
C	GLU269
C	PHE271
C	HOH418
C	HOH437

---

site_id	AC7
Number of Residues	8
Details	BINDING SITE FOR RESIDUE 0TO C 302

Chain	Residue
C	PHE53
C	LYS55
C	GLU68
C	ALA100
C	ILE101
C	GLY103
C	THR105
C	ILE215

---

site_id	AC8
Number of Residues	2
Details	BINDING SITE FOR RESIDUE ACT C 303

Chain	Residue
C	MET79
C	PRO80

---

site_id	AC9
Number of Residues	13
Details	BINDING SITE FOR RESIDUE KAN D 301

Chain	Residue
D	GLN35
D	ASP165
D	ASP167
D	ASP198
D	ARG219
D	ASN234
D	CYS235
D	GLU238
D	ASP268
D	GLU269
D	PHE271
D	HOH403
D	HOH425

---

site_id	BC1
Number of Residues	11
Details	BINDING SITE FOR RESIDUE 0TO D 302

Chain	Residue
D	ILE40
D	PHE53
D	LYS55
D	GLU68
D	PRO82
D	ALA100
D	ILE101
D	GLY103
D	THR105
D	ILE205
D	ILE215

---

site_id	BC2
Number of Residues	11
Details	BINDING SITE FOR RESIDUE KAN E 301

Chain	Residue
E	GLU269
E	PHE271
E	GLN35
E	ASP165
E	ASP167
E	ASP198
E	ARG219
E	ASN234
E	CYS235
E	GLU238
E	ASP268

---

site_id	BC3
Number of Residues	10
Details	BINDING SITE FOR RESIDUE 0TO E 302

Chain	Residue
E	ILE40
E	PHE53
E	GLU68
E	LEU72
E	THR98
E	ALA100
E	ILE101
E	GLY103
E	ILE215
E	HOH428

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Functional Information from PROSITE/UniProt

site_id	PS00109
Number of Residues	13
Details	PROTEIN_KINASE_TYR Tyrosine protein kinases specific active-site signature. VTHgDFSLDNLIF

Chain	Residue	Details
A	VAL194-PHE206

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