Summary Structural details Experimental details Functional details Sequence Neighbor History Downloads

4F38

Crystal structure of geranylgeranylated RhoA in complex with RhoGDI in its active GPPNHP-bound form

Functional Information from GO Data

Chain	GOid	namespace	contents
A	0000166	molecular_function	nucleotide binding
A	0000902	biological_process	cell morphogenesis
A	0001822	biological_process	kidney development
A	0001998	biological_process	angiotensin-mediated vasoconstriction involved in regulation of systemic arterial blood pressure
A	0002363	biological_process	alpha-beta T cell lineage commitment
A	0003100	biological_process	regulation of systemic arterial blood pressure by endothelin
A	0003189	biological_process	aortic valve formation
A	0003924	molecular_function	GTPase activity
A	0003925	molecular_function	G protein activity
A	0005515	molecular_function	protein binding
A	0005525	molecular_function	GTP binding
A	0005634	cellular_component	nucleus
A	0005737	cellular_component	cytoplasm
A	0005739	cellular_component	mitochondrion
A	0005768	cellular_component	endosome
A	0005829	cellular_component	cytosol
A	0005856	cellular_component	cytoskeleton
A	0005886	cellular_component	plasma membrane
A	0005938	cellular_component	cell cortex
A	0006357	biological_process	regulation of transcription by RNA polymerase II
A	0007010	biological_process	cytoskeleton organization
A	0007019	biological_process	microtubule depolymerization
A	0007155	biological_process	cell adhesion
A	0007160	biological_process	cell-matrix adhesion
A	0007229	biological_process	integrin-mediated signaling pathway
A	0007264	biological_process	small GTPase-mediated signal transduction
A	0007266	biological_process	Rho protein signal transduction
A	0007519	biological_process	skeletal muscle tissue development
A	0009410	biological_process	response to xenobiotic stimulus
A	0009898	cellular_component	cytoplasmic side of plasma membrane
A	0010812	biological_process	negative regulation of cell-substrate adhesion
A	0010975	biological_process	regulation of neuron projection development
A	0010977	biological_process	negative regulation of neuron projection development
A	0016020	cellular_component	membrane
A	0016477	biological_process	cell migration
A	0016787	molecular_function	hydrolase activity
A	0017022	molecular_function	myosin binding
A	0019003	molecular_function	GDP binding
A	0019901	molecular_function	protein kinase binding
A	0019904	molecular_function	protein domain specific binding
A	0021795	biological_process	cerebral cortex cell migration
A	0021861	biological_process	forebrain radial glial cell differentiation
A	0030027	cellular_component	lamellipodium
A	0030036	biological_process	actin cytoskeleton organization
A	0030154	biological_process	cell differentiation
A	0030307	biological_process	positive regulation of cell growth
A	0030334	biological_process	regulation of cell migration
A	0030424	cellular_component	axon
A	0030425	cellular_component	dendrite
A	0030496	cellular_component	midbody
A	0030521	biological_process	androgen receptor signaling pathway
A	0030838	biological_process	positive regulation of actin filament polymerization
A	0031098	biological_process	stress-activated protein kinase signaling cascade
A	0031122	biological_process	cytoplasmic microtubule organization
A	0031982	cellular_component	vesicle
A	0032154	cellular_component	cleavage furrow
A	0032467	biological_process	positive regulation of cytokinesis
A	0032587	cellular_component	ruffle membrane
A	0032956	biological_process	regulation of actin cytoskeleton organization
A	0033144	biological_process	negative regulation of intracellular steroid hormone receptor signaling pathway
A	0033688	biological_process	regulation of osteoblast proliferation
A	0034329	biological_process	cell junction assembly
A	0034446	biological_process	substrate adhesion-dependent cell spreading
A	0035385	biological_process	Roundabout signaling pathway
A	0036089	biological_process	cleavage furrow formation
A	0038027	biological_process	apolipoprotein A-I-mediated signaling pathway
A	0042476	biological_process	odontogenesis
A	0043025	cellular_component	neuronal cell body
A	0043123	biological_process	positive regulation of canonical NF-kappaB signal transduction
A	0043124	biological_process	negative regulation of canonical NF-kappaB signal transduction
A	0043149	biological_process	stress fiber assembly
A	0043197	cellular_component	dendritic spine
A	0043296	cellular_component	apical junction complex
A	0043297	biological_process	apical junction assembly
A	0043366	biological_process	beta selection
A	0043524	biological_process	negative regulation of neuron apoptotic process
A	0043525	biological_process	positive regulation of neuron apoptotic process
A	0043542	biological_process	endothelial cell migration
A	0043931	biological_process	ossification involved in bone maturation
A	0044319	biological_process	wound healing, spreading of cells
A	0045198	biological_process	establishment of epithelial cell apical/basal polarity
A	0045665	biological_process	negative regulation of neuron differentiation
A	0045666	biological_process	positive regulation of neuron differentiation
A	0045792	biological_process	negative regulation of cell size
A	0045987	biological_process	positive regulation of smooth muscle contraction
A	0046039	biological_process	GTP metabolic process
A	0046638	biological_process	positive regulation of alpha-beta T cell differentiation
A	0050773	biological_process	regulation of dendrite development
A	0050919	biological_process	negative chemotaxis
A	0051022	molecular_function	Rho GDP-dissociation inhibitor binding
A	0051301	biological_process	cell division
A	0051496	biological_process	positive regulation of stress fiber assembly
A	0051924	biological_process	regulation of calcium ion transport
A	0051964	biological_process	negative regulation of synapse assembly
A	0061430	biological_process	bone trabecula morphogenesis
A	0070507	biological_process	regulation of microtubule cytoskeleton organization
A	0071222	biological_process	cellular response to lipopolysaccharide
A	0071345	biological_process	cellular response to cytokine stimulus
A	0071526	biological_process	semaphorin-plexin signaling pathway
A	0071803	biological_process	positive regulation of podosome assembly
A	0071902	biological_process	positive regulation of protein serine/threonine kinase activity
A	0071944	cellular_component	cell periphery
A	0090051	biological_process	negative regulation of cell migration involved in sprouting angiogenesis
A	0090307	biological_process	mitotic spindle assembly
A	0090324	biological_process	negative regulation of oxidative phosphorylation
A	0097498	biological_process	endothelial tube lumen extension
A	0098794	cellular_component	postsynapse
A	0098978	cellular_component	glutamatergic synapse
A	0099159	biological_process	regulation of modification of postsynaptic structure
A	1902766	biological_process	skeletal muscle satellite cell migration
A	1903427	biological_process	negative regulation of reactive oxygen species biosynthetic process
A	1903673	biological_process	mitotic cleavage furrow formation
A	1904695	biological_process	positive regulation of vascular associated smooth muscle contraction
A	1904706	biological_process	negative regulation of vascular associated smooth muscle cell proliferation
A	1904753	biological_process	negative regulation of vascular associated smooth muscle cell migration
A	1904996	biological_process	positive regulation of leukocyte adhesion to vascular endothelial cell
A	1905274	biological_process	regulation of modification of postsynaptic actin cytoskeleton
A	1990869	biological_process	cellular response to chemokine
A	2000177	biological_process	regulation of neural precursor cell proliferation
A	2000406	biological_process	positive regulation of T cell migration
B	0001772	cellular_component	immunological synapse
B	0005094	molecular_function	Rho GDP-dissociation inhibitor activity
B	0005096	molecular_function	GTPase activator activity
B	0005504	molecular_function	fatty acid binding
B	0005515	molecular_function	protein binding
B	0005615	cellular_component	extracellular space
B	0005634	cellular_component	nucleus
B	0005737	cellular_component	cytoplasm
B	0005829	cellular_component	cytosol
B	0007266	biological_process	Rho protein signal transduction
B	0008360	biological_process	regulation of cell shape
B	0016020	cellular_component	membrane
B	0019901	molecular_function	protein kinase binding
B	0030336	biological_process	negative regulation of cell migration
B	0031267	molecular_function	small GTPase binding
B	0032880	biological_process	regulation of protein localization
B	0035023	biological_process	regulation of Rho protein signal transduction
B	0045202	cellular_component	synapse
B	0051879	molecular_function	Hsp90 protein binding
B	0071260	biological_process	cellular response to mechanical stimulus
B	0071526	biological_process	semaphorin-plexin signaling pathway
B	0098685	cellular_component	Schaffer collateral - CA1 synapse
B	0098693	biological_process	regulation of synaptic vesicle cycle

Functional Information from PDB Data

site_id	AC1
Number of Residues	12
Details	BINDING SITE FOR RESIDUE GER A 201

Chain	Residue
A	GLY189
B	TYR175
B	ILE177
B	LEU196
A	CYS190
B	LEU75
B	LEU77
B	TYR110
B	GLN130
B	ILE139
B	ASP140
B	PRO166

site_id	AC2
Number of Residues	6
Details	BINDING SITE FOR RESIDUE MG A 202

Chain	Residue
A	THR19
A	THR37
A	ASP59
A	THR60
A	GNP204
A	HOH301

site_id	AC3
Number of Residues	4
Details	BINDING SITE FOR RESIDUE MG A 203

Chain	Residue
A	ASP45
A	GLU54
A	HIS126
A	HOH335

site_id	AC4
Number of Residues	20
Details	BINDING SITE FOR RESIDUE GNP A 204

Chain	Residue
A	ILE4
A	GLY14
A	ALA15
A	CYS16
A	GLY17
A	LYS18
A	THR19
A	CYS20
A	VAL35
A	THR37
A	GLN52
A	GLN63
A	LYS118
A	ASP120
A	SER160
A	ALA161
A	LYS162
A	MG202
A	HOH301
A	HOH306

Functional Information from SwissProt/UniProt

site_id	SWS_FT_FI1
Number of Residues	17
Details	Region: {"description":"Switch II region; involved in RAP1GDS1 isoform 3 binding","evidences":[{"source":"UniProtKB","id":"P61586","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI2
Number of Residues	8
Details	Motif: {"description":"Effector region","evidences":[{"evidenceCode":"ECO:0000255"}]}

Chain

Residue

Details

site_id	SWS_FT_FI3
Number of Residues	10
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P61586","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI4
Number of Residues	13
Details	Binding site: {"evidences":[{"source":"UniProtKB","id":"P62820","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI5
Number of Residues	1
Details	Modified residue: {"description":"5-glutamyl serotonin","evidences":[{"source":"PubMed","id":"14697203","evidenceCode":"ECO:0000305"}]}

Chain

Residue

Details

site_id	SWS_FT_FI6
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine; by PKG/PRKG1","evidences":[{"source":"UniProtKB","id":"P61589","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI7
Number of Residues	1
Details	Modified residue: {"description":"Cysteine methyl ester","evidences":[{"source":"UniProtKB","id":"P61585","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI8
Number of Residues	1
Details	Lipidation: {"description":"S-geranylgeranyl cysteine","evidences":[{"source":"UniProtKB","id":"P61585","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI9
Number of Residues	2
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)","evidences":[{"source":"UniProtKB","id":"P61586","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI10
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"PubMed","id":"17242355","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"21183079","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI11
Number of Residues	1
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"23806337","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI12
Number of Residues	1
Details	Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P52565","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI13
Number of Residues	3
Details	Modified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P52565","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

site_id	SWS_FT_FI14
Number of Residues	1
Details	Modified residue: {"description":"N6-succinyllysine; alternate","evidences":[{"source":"PubMed","id":"23806337","evidenceCode":"ECO:0007744"}]}

Chain

Residue

Details

site_id	SWS_FT_FI15
Number of Residues	3
Details	Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternate","evidences":[{"source":"UniProtKB","id":"P52565","evidenceCode":"ECO:0000250"}]}

Chain

Residue

Details

		Sequence (fasta)
		PDBx/mmCIF
		PDBML format (no-atom)
		PDB format (full)
		Validation report (PDF)
		EDMap 2fo-fc (MTZ)