4F38
Crystal structure of geranylgeranylated RhoA in complex with RhoGDI in its active GPPNHP-bound form
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-11-25 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 46.000, 71.576, 136.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 68.010 - 2.800 |
R-factor | 0.20579 |
Rwork | 0.202 |
R-free | 0.28133 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1doa |
RMSD bond length | 0.015 |
RMSD bond angle | 1.743 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASES |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 68.010 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.069 | 0.265 |
Number of reflections | 20741 | |
<I/σ(I)> | 12.33 | 3.6 |
Completeness [%] | 97.0 | 98.1 |
Redundancy | 2.7 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 20% (w/v) PEG 3350, 0.2M MgCl2, 0.1M Hepes-NaOH, 25mM Hepes-NaOH pH 7.2, 40mM NaCl, 3mM MgCl2, 10M GMPPNP, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K |