4EZ8
Crystal structure of mouse thymidylate sythase in ternary complex with N(4)-hydroxy-2'-deoxycytidine-5'-monophosphate and the cofactor product, dihydrofolate
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000900 | molecular_function | mRNA regulatory element binding translation repressor activity |
| A | 0003729 | molecular_function | mRNA binding |
| A | 0004146 | molecular_function | dihydrofolate reductase activity |
| A | 0004799 | molecular_function | thymidylate synthase activity |
| A | 0005542 | molecular_function | folic acid binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005743 | cellular_component | mitochondrial inner membrane |
| A | 0005759 | cellular_component | mitochondrial matrix |
| A | 0005829 | cellular_component | cytosol |
| A | 0006206 | biological_process | pyrimidine nucleobase metabolic process |
| A | 0006231 | biological_process | dTMP biosynthetic process |
| A | 0006235 | biological_process | dTTP biosynthetic process |
| A | 0006417 | biological_process | regulation of translation |
| A | 0007623 | biological_process | circadian rhythm |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0009165 | biological_process | nucleotide biosynthetic process |
| A | 0009410 | biological_process | response to xenobiotic stimulus |
| A | 0009636 | biological_process | response to toxic substance |
| A | 0014070 | biological_process | response to organic cyclic compound |
| A | 0016020 | cellular_component | membrane |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| A | 0017148 | biological_process | negative regulation of translation |
| A | 0019860 | biological_process | uracil metabolic process |
| A | 0032259 | biological_process | methylation |
| A | 0032570 | biological_process | response to progesterone |
| A | 0033189 | biological_process | response to vitamin A |
| A | 0034097 | biological_process | response to cytokine |
| A | 0035999 | biological_process | tetrahydrofolate interconversion |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0045471 | biological_process | response to ethanol |
| A | 0046653 | biological_process | tetrahydrofolate metabolic process |
| A | 0046683 | biological_process | response to organophosphorus |
| A | 0048589 | biological_process | developmental growth |
| A | 0051216 | biological_process | cartilage development |
| A | 0051384 | biological_process | response to glucocorticoid |
| A | 0051593 | biological_process | response to folic acid |
| A | 0060574 | biological_process | intestinal epithelial cell maturation |
| A | 0097421 | biological_process | liver regeneration |
| A | 1901363 | molecular_function | heterocyclic compound binding |
| A | 1990825 | molecular_function | sequence-specific mRNA binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE NOH A 401 |
| Chain | Residue |
| A | ARG44 |
| A | SER210 |
| A | GLY211 |
| A | ASP212 |
| A | ASN220 |
| A | HIS250 |
| A | TYR252 |
| A | DHF402 |
| A | HOH510 |
| A | HOH534 |
| A | HOH564 |
| A | TRP103 |
| A | HOH692 |
| A | ARG169 |
| A | ARG170 |
| A | LEU186 |
| A | CYS189 |
| A | HIS190 |
| A | GLN208 |
| A | ARG209 |
| site_id | AC2 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE DHF A 402 |
| Chain | Residue |
| A | PHE74 |
| A | ILE102 |
| A | TRP103 |
| A | ASN106 |
| A | ASP212 |
| A | LEU215 |
| A | GLY216 |
| A | MET305 |
| A | ALA306 |
| A | NOH401 |
| A | HOH515 |
| A | HOH540 |
| A | HOH559 |
| A | HOH564 |
| A | HOH609 |
| A | HOH633 |
| A | HOH652 |
| A | HOH692 |
| A | HOH838 |
| A | HOH898 |
| A | HOH936 |
| A | HOH957 |
| A | HOH958 |
| site_id | AC3 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL A 403 |
| Chain | Residue |
| A | LYS41 |
| A | GLU42 |
| A | ASP167 |
| A | VAL198 |
| A | ASN199 |
| A | HOH525 |
| A | HOH636 |
| A | HOH721 |
| A | HOH978 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL A 404 |
| Chain | Residue |
| A | TYR295 |
| A | HOH539 |
| A | HOH614 |
| A | HOH695 |
| A | HOH850 |
Functional Information from PROSITE/UniProt
| site_id | PS00091 |
| Number of Residues | 29 |
| Details | THYMIDYLATE_SYNTHASE Thymidylate synthase active site. RriImcaWNpkdlplma.....LpPCHalcQFyV |
| Chain | Residue | Details |
| A | ARG169-VAL197 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | ACT_SITE: Nucleophile => ECO:0000250|UniProtKB:P0A884 |
| Chain | Residue | Details |
| A | CYS189 |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | BINDING: in other chain => ECO:0000250|UniProtKB:P0A884 |
| Chain | Residue | Details |
| A | ARG44 | |
| A | ASN220 |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P45352 |
| Chain | Residue | Details |
| A | ARG169 |
| site_id | SWS_FT_FI4 |
| Number of Residues | 3 |
| Details | BINDING: in other chain => ECO:0000250|UniProtKB:P45352 |
| Chain | Residue | Details |
| A | CYS189 | |
| A | ARG209 | |
| A | HIS250 |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | BINDING: BINDING => ECO:0000250|UniProtKB:P0A884 |
| Chain | Residue | Details |
| A | ASP212 | |
| A | ALA306 |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | MOD_RES: Phosphoserine => ECO:0000250|UniProtKB:P04818 |
| Chain | Residue | Details |
| A | SER108 |
| site_id | SWS_FT_FI7 |
| Number of Residues | 3 |
| Details | CROSSLNK: Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2) => ECO:0000250|UniProtKB:P04818 |
| Chain | Residue | Details |
| A | LYS286 | |
| A | LYS302 |
