4EZ8
Crystal structure of mouse thymidylate sythase in ternary complex with N(4)-hydroxy-2'-deoxycytidine-5'-monophosphate and the cofactor product, dihydrofolate
Functional Information from GO Data
| Chain | GOid | namespace | contents |
| A | 0000900 | molecular_function | mRNA regulatory element binding translation repressor activity |
| A | 0003729 | molecular_function | mRNA binding |
| A | 0004799 | molecular_function | thymidylate synthase activity |
| A | 0005542 | molecular_function | folic acid binding |
| A | 0005634 | cellular_component | nucleus |
| A | 0005657 | cellular_component | replication fork |
| A | 0005737 | cellular_component | cytoplasm |
| A | 0005739 | cellular_component | mitochondrion |
| A | 0005743 | cellular_component | mitochondrial inner membrane |
| A | 0005759 | cellular_component | mitochondrial matrix |
| A | 0005829 | cellular_component | cytosol |
| A | 0006207 | biological_process | 'de novo' pyrimidine nucleobase biosynthetic process |
| A | 0006231 | biological_process | dTMP biosynthetic process |
| A | 0006235 | biological_process | dTTP biosynthetic process |
| A | 0006260 | biological_process | DNA replication |
| A | 0006417 | biological_process | regulation of translation |
| A | 0008168 | molecular_function | methyltransferase activity |
| A | 0009165 | biological_process | nucleotide biosynthetic process |
| A | 0016740 | molecular_function | transferase activity |
| A | 0016741 | molecular_function | transferase activity, transferring one-carbon groups |
| A | 0017148 | biological_process | negative regulation of translation |
| A | 0032259 | biological_process | methylation |
| A | 0035999 | biological_process | tetrahydrofolate interconversion |
| A | 0042803 | molecular_function | protein homodimerization activity |
| A | 0046653 | biological_process | tetrahydrofolate metabolic process |
| A | 1901363 | molecular_function | heterocyclic compound binding |
| A | 1990825 | molecular_function | sequence-specific mRNA binding |
Functional Information from PDB Data
| site_id | AC1 |
| Number of Residues | 20 |
| Details | BINDING SITE FOR RESIDUE NOH A 401 |
| Chain | Residue |
| A | ARG44 |
| A | SER210 |
| A | GLY211 |
| A | ASP212 |
| A | ASN220 |
| A | HIS250 |
| A | TYR252 |
| A | DHF402 |
| A | HOH510 |
| A | HOH534 |
| A | HOH564 |
| A | TRP103 |
| A | HOH692 |
| A | ARG169 |
| A | ARG170 |
| A | LEU186 |
| A | CYS189 |
| A | HIS190 |
| A | GLN208 |
| A | ARG209 |
| site_id | AC2 |
| Number of Residues | 23 |
| Details | BINDING SITE FOR RESIDUE DHF A 402 |
| Chain | Residue |
| A | PHE74 |
| A | ILE102 |
| A | TRP103 |
| A | ASN106 |
| A | ASP212 |
| A | LEU215 |
| A | GLY216 |
| A | MET305 |
| A | ALA306 |
| A | NOH401 |
| A | HOH515 |
| A | HOH540 |
| A | HOH559 |
| A | HOH564 |
| A | HOH609 |
| A | HOH633 |
| A | HOH652 |
| A | HOH692 |
| A | HOH838 |
| A | HOH898 |
| A | HOH936 |
| A | HOH957 |
| A | HOH958 |
| site_id | AC3 |
| Number of Residues | 9 |
| Details | BINDING SITE FOR RESIDUE GOL A 403 |
| Chain | Residue |
| A | LYS41 |
| A | GLU42 |
| A | ASP167 |
| A | VAL198 |
| A | ASN199 |
| A | HOH525 |
| A | HOH636 |
| A | HOH721 |
| A | HOH978 |
| site_id | AC4 |
| Number of Residues | 5 |
| Details | BINDING SITE FOR RESIDUE GOL A 404 |
| Chain | Residue |
| A | TYR295 |
| A | HOH539 |
| A | HOH614 |
| A | HOH695 |
| A | HOH850 |
Functional Information from PROSITE/UniProt
| site_id | PS00091 |
| Number of Residues | 29 |
| Details | THYMIDYLATE_SYNTHASE Thymidylate synthase active site. RriImcaWNpkdlplma.....LpPCHalcQFyV |
| Chain | Residue | Details |
| A | ARG169-VAL197 |
Functional Information from SwissProt/UniProt
| site_id | SWS_FT_FI1 |
| Number of Residues | 1 |
| Details | Active site: {"description":"Nucleophile","evidences":[{"source":"UniProtKB","id":"P0A884","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI2 |
| Number of Residues | 2 |
| Details | Binding site: {"description":"in other chain","evidences":[{"source":"UniProtKB","id":"P0A884","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI3 |
| Number of Residues | 1 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"P45352","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI4 |
| Number of Residues | 6 |
| Details | Binding site: {"description":"in other chain","evidences":[{"source":"UniProtKB","id":"P45352","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI5 |
| Number of Residues | 2 |
| Details | Binding site: {"evidences":[{"source":"UniProtKB","id":"P0A884","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI6 |
| Number of Residues | 1 |
| Details | Modified residue: {"description":"Phosphoserine","evidences":[{"source":"UniProtKB","id":"P04818","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
| site_id | SWS_FT_FI7 |
| Number of Residues | 3 |
| Details | Cross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"UniProtKB","id":"P04818","evidenceCode":"ECO:0000250"}]} |
| Chain | Residue | Details |
